Abstract
The cell walls of selected bacteria were studied by X-ray diffraction analysis to determine and characterize crystalline components. The walls were isolated by mechanical disruption and purified by enzymatic and washing procedures. The X-ray diffraction lines which appeared from the gram-positive cell walls were shown to be due to the constituent “mucopeptide” fraction. No diffraction lines could be obtained from the gram-negative bacterium studied. The results show that crystallinity is associated with mucopeptide.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARTNICKI-GARCIA S., NICKERSON W. J. Isolation, composition, and structure of cell walls of filamentous and yeast-like forms of Mucor rouxii. Biochim Biophys Acta. 1962 Mar 26;58:102–119. doi: 10.1016/0006-3002(62)90822-3. [DOI] [PubMed] [Google Scholar]
- CUMMINS C. S., HARRIS H. The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character. J Gen Microbiol. 1956 Jul;14(3):583–600. doi: 10.1099/00221287-14-3-583. [DOI] [PubMed] [Google Scholar]
- HOUWINK A. L., KREGER D. R. Observations on the cell wall of yeasts; an electron microscope and x-ray diffraction study. Antonie Van Leeuwenhoek. 1953;19(1):1–24. doi: 10.1007/BF02594830. [DOI] [PubMed] [Google Scholar]
- KORN E. D., NORTHCOTE D. H. Physical and chemical properties of polysaccharides and glycoproteins of the yeast-cell wall. Biochem J. 1960 Apr;75:12–17. doi: 10.1042/bj0750012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KREGER D. R. Observations on cell walls of yeasts and some other fungi by x-ray diffraction and solubility tests. Biochim Biophys Acta. 1954 Jan;13(1):1–9. doi: 10.1016/0006-3002(54)90264-4. [DOI] [PubMed] [Google Scholar]
- MANDELSTAM J., ROGERS H. J. The incorporation of amino acids into the cell-wall mucopeptide of staphylococci and the effect of antibiotics on the process. Biochem J. 1959 Aug;72:654–662. doi: 10.1042/bj0720654. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MYERS A., PRESTON R. D. Fine structure in the red algae. III. A general survey of cell-wall structure in the red algae. Proc R Soc Lond B Biol Sci. 1959 Sep 1;150:456–459. doi: 10.1098/rspb.1959.0034. [DOI] [PubMed] [Google Scholar]
- ROELOFSEN P. A., DALITZ V. C., WIJNMAN C. F. Constitution, submicroscopic structure and degree of crystallinity of the cell wall of Halicystis osterhoutii. Biochim Biophys Acta. 1953 Jul;11(3):344–352. doi: 10.1016/0006-3002(53)90054-7. [DOI] [PubMed] [Google Scholar]
- SALTON M. R. Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim Biophys Acta. 1953 Apr;10(4):512–523. doi: 10.1016/0006-3002(53)90296-0. [DOI] [PubMed] [Google Scholar]
- SALTON M. R. The lysis of micro-organisms by lysozyme and related enzymes. J Gen Microbiol. 1958 Apr;18(2):481–490. doi: 10.1099/00221287-18-2-481. [DOI] [PubMed] [Google Scholar]