Abstract
The amount of thiobarbiturate-reacting material in 51 strains of bacteria and three yeasts was determined. Reactive material was found to be present in all of the gram-negative bacteria examined. It was assumed that the reactive material in this case was primarily 2-keto-3-deoxyoctonate (KDO), an eight-carbon sugar acid which is usually associated with the cell wall lipolysaccharide of members of the Salmonella-Escherichia group. Very little reactive material could be detected in the gram-positive species and yeasts that were examined. When expressed as per cent dry weight, the gram-negative bacteria exhibited about eight times more reactive material than the gram-positive species. It is suggested that the small amount of reactive material detected in gram-positive cells and yeasts is due to compounds other than KDO.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Edstrom R. D., Heath E. C. Sugar nucleotide transferases in Escherichia coli lipopolysaccharide biosynthesis. Biochem Biophys Res Commun. 1964 Aug 11;16(6):576–581. doi: 10.1016/0006-291x(64)90195-0. [DOI] [PubMed] [Google Scholar]
- Heath E. C., Mayer R. M., Edstrom R. D., Beaudreau C. A. Structure and biosynthesis of the cell wall lipopolysaccharide of Escherichia coli. Ann N Y Acad Sci. 1966 Jun 30;133(2):315–333. doi: 10.1111/j.1749-6632.1966.tb52374.x. [DOI] [PubMed] [Google Scholar]
- Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OSBORN M. J., ROSEN S. M., ROTHFIELD L., ZELEZNICK L. D., HORECKER B. L. LIPOPOLYSACCHARIDE OF THE GRAM-NEGATIVE CELL WALL. Science. 1964 Aug 21;145(3634):783–789. doi: 10.1126/science.145.3634.783. [DOI] [PubMed] [Google Scholar]
- OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rapin A. M., Mayer H. Complex polysaccharides in different strains of Escherichia coli K-12. Ann N Y Acad Sci. 1966 Jun 30;133(2):425–437. doi: 10.1111/j.1749-6632.1966.tb52381.x. [DOI] [PubMed] [Google Scholar]
- WARAVDEKAR V. S., SASLAW L. D. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction. J Biol Chem. 1959 Aug;234(8):1945–1950. [PubMed] [Google Scholar]
- WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]
