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. 1967 Jan;93(1):237–244. doi: 10.1128/jb.93.1.237-244.1967

Genetic and Biochemical Analysis of the Isoenzymes Concerned in the First Reaction of Aromatic Biosynthesis in Escherichia coli

B J Wallace 1, James Pittard 1
PMCID: PMC314994  PMID: 5335890

Abstract

Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of these activities have been mapped and are widely separated from each other on the E. coli chromosome. Chromatography on diethylaminoethyl cellulose columns allowed the recognition of four peaks of activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADELBERG E. A., MYERS J. W. Modification of the penicillin technique for the selection of auxotrophic bacteria. J Bacteriol. 1953 Mar;65(3):348–353. doi: 10.1128/jb.65.3.348-353.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROWN K. D., DOY C. H. END-PRODUCT REGULATION OF THE GENERAL AROMATIC-PATHWAY IN ESCHERICHIA COLI W. Biochim Biophys Acta. 1963 Sep 3;77:170–172. doi: 10.1016/0006-3002(63)90489-x. [DOI] [PubMed] [Google Scholar]
  4. Brown K. D., Doy C. H. Control of three isoenzymic 7-phospho-2-oxo-3-deoxy-Darabino-heptonate-D-erythrose-4-phosphate lyases of Escherichia coli W and derived mutants by repressive and "inductive" effects of the aromatic amino acids. Biochim Biophys Acta. 1966 Apr 12;118(1):157–172. doi: 10.1016/s0926-6593(66)80153-4. [DOI] [PubMed] [Google Scholar]
  5. COHEN G. N., PATTE J. C., TRUFFA-BACHI P. PARALLEL MODIFICATIONS CAUSED BY MUTATIONS IN TWO ENZYMES CONCERNED WITH THE BIOSYNTHESIS OF THREONINE IN ESCHERICHIA COLI. Biochem Biophys Res Commun. 1965 May 3;19:546–550. doi: 10.1016/0006-291x(65)90160-9. [DOI] [PubMed] [Google Scholar]
  6. Doy C. H., Brown K. D. Control of aromatic biosynthesis: the multiplicity of 7-phospho-2-oxo-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating) in Escherichia coli W. Biochim Biophys Acta. 1965 Jul 8;104(2):377–389. doi: 10.1016/0304-4165(65)90343-0. [DOI] [PubMed] [Google Scholar]
  7. EZEKIEL D. H. FALSE FEEDBACK INHIBITION OF AROMATIC AMINO ACID BIOSYNTHESIS BY BETA-2-THIENYLALANINE. Biochim Biophys Acta. 1965 Jan 11;95:54–62. doi: 10.1016/0005-2787(65)90210-8. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Morse M L, Lederberg E M, Lederberg J. Transduction in Escherichia Coli K-12. Genetics. 1956 Jan;41(1):142–156. doi: 10.1093/genetics/41.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NOVICK A., HORIUCHI T. Hyper-production of beta-galactosidase by Escherichia coli bacteria. Cold Spring Harb Symp Quant Biol. 1961;26:239–245. doi: 10.1101/sqb.1961.026.01.029. [DOI] [PubMed] [Google Scholar]
  11. Patte J. C., Cohen G. N. Isolement et propriétés d'un mutant d'Escherichia coli depourvu d'aspartokinase sensible à la lysine. Biochim Biophys Acta. 1965 Jun 22;99(3):561–563. [PubMed] [Google Scholar]
  12. Pittard J., Wallace B. J. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J Bacteriol. 1966 Apr;91(4):1494–1508. doi: 10.1128/jb.91.4.1494-1508.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SMITH L. C., RAVEL J. M., LAX S. R., SHIVE W. The control of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthesis by phenylalanine and tyrosine. J Biol Chem. 1962 Nov;237:3566–3570. [PubMed] [Google Scholar]
  14. SRINIVASAN P. R., SPRINSON D. B. 2-Keto-3-deoxy-D-arabo-heptonic acid 7-phosphate synthetase. J Biol Chem. 1959 Apr;234(4):716–722. [PubMed] [Google Scholar]

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