Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Jan;93(1):273–277. doi: 10.1128/jb.93.1.273-277.1967

Cell Wall Constituents of Leuconostoc citrovorum and Leuconostoc mesenteroides1

Sheila J Harney a, Nicholas D Simopoulos a, Miyoshi Ikawa a
PMCID: PMC314998  PMID: 6020410

Abstract

The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal amino acids and amino sugars in all three strains. In addition, strain 10830a contained l-serine as a major cell wall component. Quantitative amino acid analyses indicate that glutamic acid, lysine, glucosamine, muramic acid, and serine may be present in the cell walls in equimolar amounts and that alanine is present in three to four times these quantities. The similarities and differences between the cell wall constituents of the leuconostocs and those of the lactobacilli and streptococci are discussed.

Full text

PDF
273

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG J. J., BADDILEY J., BUCHANAN J. G., DAVISION A. L., KELEMEN M. V., NEUHAUS F. C. Composition of teichoic acids from a number of bacterial walls. Nature. 1959 Jul 25;184:247–248. doi: 10.1038/184247a0. [DOI] [PubMed] [Google Scholar]
  2. BADDILEY J., DAVISON A. L. The occurrence and location of teichoic acids in lactobacilli. J Gen Microbiol. 1961 Feb;24:295–299. doi: 10.1099/00221287-24-2-295. [DOI] [PubMed] [Google Scholar]
  3. BAIRD-PARKER A. C. THE CLASSIFICATION OF STAPHYLOCOCCI AND MICROCOCCI FROM WORLD-WIDE SOURCES. J Gen Microbiol. 1965 Mar;38:363–387. doi: 10.1099/00221287-38-3-363. [DOI] [PubMed] [Google Scholar]
  4. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  5. CUMMINS C. S., HARRIS H. The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character. J Gen Microbiol. 1956 Jul;14(3):583–600. doi: 10.1099/00221287-14-3-583. [DOI] [PubMed] [Google Scholar]
  6. Colman G., Williams R. E. The cell walls of streptococci. J Gen Microbiol. 1965 Dec;41(3):375–387. doi: 10.1099/00221287-41-3-375. [DOI] [PubMed] [Google Scholar]
  7. GARVIE E. I. Reclassification of Leuconostoc mesenteroides P. 60 as a Pedicoccus. Nature. 1959 May 16;183(4672):1411–1412. doi: 10.1038/1831411a0. [DOI] [PubMed] [Google Scholar]
  8. IKAWA M. NATURE OF THE LIPIDS OF SOME LACTIC ACID BACTERIA. J Bacteriol. 1963 Apr;85:772–781. doi: 10.1128/jb.85.4.772-781.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. IKAWA M., PETTAS I. CELL-WALL CONSTITUENTS OF LACTOBACILLUS DELBRUECKII. J Bacteriol. 1963 Sep;86:609–610. doi: 10.1128/jb.86.3.609-610.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. IKAWA M., SNELL E. E. Cell wall composition of lactic acid bacteria. J Biol Chem. 1960 May;235:1376–1382. [PubMed] [Google Scholar]
  11. McCLESKEY C. S. The identity of strain P-60 of Leuconostoc mesenteroides. J Bacteriol. 1952 Jul;64(1):140–141. doi: 10.1128/jb.64.1.140-141.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PIEZ K. A., MORRIS L. A modified procedure for the automatic analysis of amino acids. Anal Biochem. 1960 Nov;1:187–201. doi: 10.1016/0003-2697(60)90045-2. [DOI] [PubMed] [Google Scholar]
  13. SALTON M. R., PAVLIK J. G. Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme. Biochim Biophys Acta. 1960 Apr 22;39:398–407. doi: 10.1016/0006-3002(60)90191-8. [DOI] [PubMed] [Google Scholar]
  14. SEIFTER S., DAYTON S. The estimation of glycogen with the anthrone reagent. Arch Biochem. 1950 Jan;25(1):191–200. [PubMed] [Google Scholar]
  15. SHARPE M. E., DAVISON A. L., BADDILEY J. TEICHOIC ACIDS AND GROUP ANTIGENS IN LACTOBACILLI. J Gen Microbiol. 1964 Feb;34:333–340. doi: 10.1099/00221287-34-2-333. [DOI] [PubMed] [Google Scholar]
  16. SLADE H. D., SLAMP W. C. Cell-wall composition and the grouping antigens of Streptococci. J Bacteriol. 1962 Aug;84:345–351. doi: 10.1128/jb.84.2.345-351.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WICKEN A. J., ELLIOTT S. D., BADDILEY J. The identity of streptococcal group D antigen with teichoic acid. J Gen Microbiol. 1963 May;31:231–239. doi: 10.1099/00221287-31-2-231. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES