Abstract
Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK′ values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was chosen as possessing suitable physical properties, cost, and purity for biological use. Aerobacter aerogenes and Escherichia coli grew normally in its presence but did not utilize it as a sole source and energy. Although good turbidimetric data could not be obtained, Pseudomonas aeruginosa responded in the same qualitative way. Therefore, EMTA promises to be useful in work on endogenous metabolism and in nutritional studies.
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Selected References
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- Bohinski R. C., Mallette M. F. Behavior of Escherichia coli B in sulfate-limited medium. Can J Microbiol. 1965 Aug;11(4):663–669. doi: 10.1139/m65-089. [DOI] [PubMed] [Google Scholar]
- Bohinski R. C., Mallette M. F. Response of log-phase cells of Escherichia coli to medium limited in both sulfate and phosphate. J Bacteriol. 1967 Apr;93(4):1316–1326. doi: 10.1128/jb.93.4.1316-1326.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Good N. E., Winget G. D., Winter W., Connolly T. N., Izawa S., Singh R. M. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467–477. doi: 10.1021/bi00866a011. [DOI] [PubMed] [Google Scholar]
- Hou C. I., Gronlund A. F., Campbell J. J. Influence of phosphate starvation on cultures of Pseudomonas aeruginosa. J Bacteriol. 1966 Oct;92(4):851–855. doi: 10.1128/jb.92.4.851-855.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lewis J. C. Mono- and bis(2-hydroxyethyl)imino-tris(hydroxymethyl)-methane, "mono-tris" and "bis-tris": new buffer bases with pKa t 7.83 and 6.46. Anal Biochem. 1966 Mar;14(3):495–496. doi: 10.1016/0003-2697(66)90294-6. [DOI] [PubMed] [Google Scholar]
- MALLETTE M. F., COWAN C. I., CAMPBELL J. J. GROWTH AND SURVIVAL OF ESCHERICHIA COLI IN MEDIUM LIMITED IN PHOSPHATE. J Bacteriol. 1964 Apr;87:779–785. doi: 10.1128/jb.87.4.779-785.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]