Abstract
The methionine analogue, α-methylmethionine, inhibits bacterial growth, but its action is overcome by methionine, homocysteine, and cystathionine. The effect of the analogue on growth is attributed to its ability to mimic methionine as a feed-back inhibitor of the first enzyme specific to methionine biosynthesis. This conclusion is based on the findings that (i) α-methylmethionine inhibits excretion of O-succinylhomoserine, the product of the first enzyme, by a methionine auxotroph unable to convert succinylhomoserine to cystahionine, and that (ii) the enzyme homoserine O-transsuccinylase is inhibited by α-methylmethionine in extracts of Escherichia coli. α-Methylmethionine also inhibits methionyl-ribonucleic acid synthetase in extracts, but this inhibition probably does not affect growth.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balish E., Shapiro S. K. Cystathionine as a precursor of methionine in Escherichia coli and Aerobacter aerogenes. J Bacteriol. 1966 Nov;92(5):1331–1336. doi: 10.1128/jb.92.5.1331-1336.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark F. C., Marcker K. A. Coding response of N-fromyl-methionyl-sRNA to UUG. Nature. 1965 Sep 4;207(5001):1038–1039. doi: 10.1038/2071038b0. [DOI] [PubMed] [Google Scholar]
- Cohen G. N. Regulation of enzyme activity in microorganisms. Annu Rev Microbiol. 1965;19:105–126. doi: 10.1146/annurev.mi.19.100165.000541. [DOI] [PubMed] [Google Scholar]
- DELAVIER-KLUTCHKO C., FLAVIN M. ENZYMATIC SYNTHESIS AND CLEAVAGE OF CYSTATHIONINE IN FUNGI AND BACTERIA. J Biol Chem. 1965 Jun;240:2537–2549. [PubMed] [Google Scholar]
- Kaplan M. M., Flavin M. Cystathionine gamma-synthetase of Salmonella. Catalytic properties of a new enzyme in bacterial methionine biosynthesis. J Biol Chem. 1966 Oct 10;241(19):4463–4471. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lee L. W., Ravel J. M., Shive W. Multimetabolite control of a biosynthetic pathway by sequential metabolites. J Biol Chem. 1966 Nov 25;241(22):5479–5480. [PubMed] [Google Scholar]
- MOYED H. S., FRIEDMAN M. Interference with feedback control; a mechanism of antimetabolite action. Science. 1959 Apr 10;129(3354):968–969. doi: 10.1126/science.129.3354.968. [DOI] [PubMed] [Google Scholar]
- ROWBURY R. J. THE ACCUMULATION OF O-SUCCINYLHOMOSERINE BY ESCHERICHIA COLI AND SALMONELLA TYPHIMURIUM. J Gen Microbiol. 1964 Nov;37:171–180. doi: 10.1099/00221287-37-2-171. [DOI] [PubMed] [Google Scholar]
- ROWBURY R. J., WOODS D. D. O-SUCCINYLHOMOSERINE AS AN INTERMEDIATE IN THE SYNTHESIS OF CYSTATHIONINE BY ESCHERICHIA COLI. J Gen Microbiol. 1964 Sep;36:341–358. doi: 10.1099/00221287-36-3-341. [DOI] [PubMed] [Google Scholar]
- Rowbury R. J., Woods D. D. The regulation of cystathionine formation in Escherichia coli. J Gen Microbiol. 1966 Jan;42(1):155–163. doi: 10.1099/00221287-42-1-155. [DOI] [PubMed] [Google Scholar]