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. Author manuscript; available in PMC: 2011 Aug 4.
Published in final edited form as: AIDS. 2010 Jun 1;24(9):1251–1258. doi: 10.1097/QAD.0b013e3283389e90

Fig. 3. Casp8p41 expression increases tumor necrosis factor.

Fig. 3

(a) Primary CD4 T cells were transfected with HA, HACasp8p41 or HACasp8p41ΔDED and analyzed by western blot for tumor necrosis factor (TNF) [recombinant TNF (rTNF)]. Control blotting with anti-HA and antitubulin was performed. (b) Primary human CD4 T cells were infected with HIVIIIb and 3 days after infection were analyzed for Casp8p41 and TNF by flow cytometry. Isotype control antibodies were used to determine TNF positivity. (c) The specificity of TNF production in Casp8p41-positive cells was assessed by simultaneous analysis of TNF and Casp8p41. Results are representative of four separate experiments.