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. 1967 Aug;94(2):359–364. doi: 10.1128/jb.94.2.359-364.1967

Electrophoretic Patterns of Membrane Proteins of Mycoplasma

Shlomo Rottem 1, Shmuel Razin 1
PMCID: PMC315048  PMID: 6039359

Abstract

Cell membranes of Mycoplasma were isolated either by osmotic lysis or by ultrasonic disruption of the organisms. The membranes were dissolved in phenol-acetic acid-water (2:1:0.5, w/v/v), and membrane proteins were separated electrophoretically in polyacrylamide gels containing 5 m urea and 35% (v/v) acetic acid. The electrophoretic patterns of membrane proteins were highly specific for the different Mycoplasma strains examined. The use of this method to prove the identity or dissimilarity of Mycoplasma strains is suggested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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