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. 1967 Aug;94(2):422–433. doi: 10.1128/jb.94.2.422-433.1967

Fine Structure of the Cytomembranes of Nitrosocystis oceanus1

Charles C Remsen 1,2,2, Frederica W Valois 1,2, Stanley W Watson 1,2
PMCID: PMC315057  PMID: 4292313

Abstract

Thin-sectioned, negatively stained, and freeze-etched preparations of Nitrosocystis oceanus cytomembranes were compared. The cytomembranes in freeze-etched cells were covered with 80- to 120-A particles. When cells were disrupted and differentially centrifuged, various membrane and particle fractions were obtained. Negatively stained membrane fragments from the pellet centrifuged at 3,000 × g showed 70- to 80-A stalked particles, whereas those from the pellet centrifuged at 39,000 × g exhibited a crystalline array of subunits with a 30- to 40-A periodicity. High-speed supernatant and pellet fractions centrifuged at greater than 39,000 × g contained 40- to 120-A free particles but no membranes. In chemically fixed cells, 40-A particles were found embedded in the matrix of membranes. Results suggest that the larger 80- to 120-A particles are enzyme complexes, whereas the smaller 30- to 40-A particles represent a structural protein or a lipoprotein of the membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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