Abstract
Previous investigations have demonstrated the existence of five Histoplasma capsulatum serotypes. Available specific fluorescent-antibody reagents stain only four of the five serotypes. Antibodies produced against the most complete H. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagent specific for H. capsulatum that was reactive with all the known serotypes. The unadsorbed reagent not only stained all the H. capsulatum serotypes, but it also stained cultures of Blastomyces dermatitidis, H. duboisii, several Candida species, and a variety of other fungi. Adsorption of the conjugate with antigens of C. albicans produced a reagent that intensely stained only H. capsulatum, H. duboisii, and B. dermatitidis. Differentiation of B. dermatitidis from the Histoplasma species was accomplished by application of a B. dermatitidis specific fluorescent antibody to antigens positive with the H. capsulatum reagent. At present, differentiation of H. capsulatum from H. duboisii may be accomplished only by animal inoculation. Our data substantiate the antigenic relationships hypothesized earlier, and they indicate that H. capsulatum shares at least two antigens with the other fungi that were studied.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GORDON M. A. Fluorescent staining of Histoplasma capsulatum. J Bacteriol. 1959 Jun;77(6):678–681. doi: 10.1128/jb.77.6.678-681.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAPLAN W., KAUFMAN L. SPECIFIC FLUORESCENT ANTIGLOBULINS FOR THE DETECTION AND IDENTIFICATION OF BLASTOMYCES DERMATITIDIS YEAST-PHASE CELLS. Mycopathol Mycol Appl. 1963 Jun 15;19:173–180. doi: 10.1007/BF02051245. [DOI] [PubMed] [Google Scholar]
- KAUFMAN L., KAPLAN W. Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum. J Bacteriol. 1961 Nov;82:729–735. doi: 10.1128/jb.82.5.729-735.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufman L., Blumer S. Occurrence of serotypes among Histoplasma capsulatum strains. J Bacteriol. 1966 Apr;91(4):1434–1439. doi: 10.1128/jb.91.4.1434-1439.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LYNCH H. J., Jr, PLEXICO K. L. A rapid method for screening sputums for Histoplasma capsulatum employing the fluorescent-antibody technic. N Engl J Med. 1962 Apr 19;266:811–814. doi: 10.1056/NEJM196204192661606. [DOI] [PubMed] [Google Scholar]