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. 1968 Apr;95(4):1388–1399. doi: 10.1128/jb.95.4.1388-1399.1968

Synthesis of Protein and Ribonucleic Acid in a Psychrophile at Normal and Restrictive Growth Temperatures

Neil L Malcolm a,1
PMCID: PMC315098  PMID: 5646626

Abstract

A defined medium was capable of supporting the growth of a psychrophilic coccus over its growth temperature range, −4 to 25 C. A rapid loss of viability occurred when exponential cells were transferred to growth-restricting temperatures above 25 C. Comparative studies of the chemistry of exponential-phase cells and cells exposed to supermaximum temperature indicated that this loss of viability is not due to temperature-induced membrane damage, inhibition of respiration or energy metabolism, or depletion of intracellular reserves. Moribund and dead cell populations showed an elevated level of intracellular adenosine-5′-triphosphate and amino acids—a finding reflected in the reduced rate of amino acid synthesis during the recovery of heat-shocked cells—and also leakage of degraded ribonucleic acid products into the medium. Incorporation studies indicated that loss of viability at 30 C was correlated with inhibition of protein synthesis, followed later by inhibition of ribonucleic acid synthesis. Deoxyribonucleic acid synthesis was unaffected by temperature above the maximum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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