Abstract
Phenol-soluble lipopolysaccharides were obtained from the interphase and phenol phase fractions of 44% aqueous phenol-extracted Citrobacter species. Upon detailed investigation of C. freundii 8090, the two lipopolysaccharide fractions were found to contain different amounts of lipid A, although qualitative composition was similar. Both contained lipid A, 2-keto-deoxyoctonic acid, heptose, phosphate, d-glucose, galactose, rhamnose, 2-acetamido-2 deoxy-d-glucose, 3-acetamido-3,6-dideoxy-d-glucose, O-acetyl, and trace amino acids. Partially purified phenol-phase lipopolysaccharide partitioned into the phenol-soluble phase when refractionated with 44% aqueous phenol, and was further found to be soluble in 88% phenol, 95% ethyl alcohol, and chloroform-methanol (2:1).
Full text
PDF








Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ashwell G., Volk W. A. Isolation and identification of N-acetyl-3-amino-3,6-dideoxy-D-galactose, a cell wall constituent of Xanthomonas campestris. J Biol Chem. 1965 Dec;240(12):4549–4555. [PubMed] [Google Scholar]
- BARRY G. T., ABBOTT V., TSAI T. Relationship of colominic acid (poly N-acetylneuraminic acid) to bacteria which contain neuraminic acid. J Gen Microbiol. 1962 Oct;29:335–352. doi: 10.1099/00221287-29-2-335. [DOI] [PubMed] [Google Scholar]
- BURTON A. J., CARTER H. E. PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI. Biochemistry. 1964 Mar;3:411–418. doi: 10.1021/bi00891a018. [DOI] [PubMed] [Google Scholar]
- Distler J., Kaufman B., Roseman S. Enzymic synthesis of a diamino sugar nucleotide by extracts of type XIV Diplococcus pneumoniae. Arch Biochem Biophys. 1966 Sep 26;116(1):466–478. doi: 10.1016/0003-9861(66)90054-3. [DOI] [PubMed] [Google Scholar]
- ELBEIN A. D., HEATH E. C. THE BIOSYNTHESIS OF CELL WALL LIPOPOLYSACCHARIDE IN ESCHERICHIA COLI. I. THE BIOCHEMICAL PROPERTIES OF A URIDINE DIPHOSPHATE GALACTOSE 4-EPIMERASELESS MUTANT. J Biol Chem. 1965 May;240:1919–1925. [PubMed] [Google Scholar]
- Freeman G. G. The preparation and properties of a specific polysaccharide from Bact. typhosum Ty(2): With an addendum by J. St L. Philpot, From the Department of Biochemistry, Oxford. Biochem J. 1942 Apr;36(3-4):340–356. doi: 10.1042/bj0360340. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hickman J., Ashwell G. Isolation of a bacterial lipopolysaccharide from Xanthomonas campestris containing 3-acetamido-3,6-dideoxy-D-galactose and D-rhamnose. J Biol Chem. 1966 Mar 25;241(6):1424–1428. [PubMed] [Google Scholar]
- Lüderitz O., Ruschmann E., Westphal O., Raff R., Wheat R. Occurrence of 3-amino-3,6-dideoxyhexoses in Salmonella and related bacteria. J Bacteriol. 1967 May;93(5):1681–1687. doi: 10.1128/jb.93.5.1681-1687.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MATSUHASHI M., STROMINGER J. L. THYMIDINE DIPHOSPHATE 4-ACETAMIDO-4, 6-DIDEOXYHEXOSES. I. ENZYMATIC SYNTHESIS BY STRAINS OF ESCHERICHIA COLI. J Biol Chem. 1964 Aug;239:2454–2463. [PubMed] [Google Scholar]
- Mergenhagen S. E., Bladen H. A., Hsu K. C. Electron microscopic localization of endotoxic lipopolysaccharide in gram-negative organisms. Ann N Y Acad Sci. 1966 Jun 30;133(2):279–291. doi: 10.1111/j.1749-6632.1966.tb52371.x. [DOI] [PubMed] [Google Scholar]
- OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raff R. A., Wheat R. W. Characterization of 3-amino-3,6-dideoxy-D-glucose from a bacterial lipopolysaccharide. J Biol Chem. 1967 Oct 25;242(20):4610–4613. [PubMed] [Google Scholar]
- Raff R. A., Wheat R. W. Occurrence of a 3-amino sugar in the cell wall of Citrobacter freundii 8090. Biochim Biophys Acta. 1966 Sep 26;127(1):271–273. doi: 10.1016/0304-4165(66)90507-1. [DOI] [PubMed] [Google Scholar]
- Rapin A. M., Mayer H. Complex polysaccharides in different strains of Escherichia coli K-12. Ann N Y Acad Sci. 1966 Jun 30;133(2):425–437. doi: 10.1111/j.1749-6632.1966.tb52381.x. [DOI] [PubMed] [Google Scholar]
- Rothfield L., Takeshita M. The role of phospholipid in the biosynthesis of cell wall lipopolysaccharide in S. typhimurium. Ann N Y Acad Sci. 1966 Jun 30;133(2):384–390. doi: 10.1111/j.1749-6632.1966.tb52378.x. [DOI] [PubMed] [Google Scholar]
- TANFORD C., MARLER E., JURY E., DAVIDSON E. A. DETERMINATION OF THE MOLECULAR WEIGHT DISTRIBUTION OF CHONDROITIN SULFATE B BY SEDIMENTATION EQUILIBRIUM. J Biol Chem. 1964 Dec;239:4034–4040. [PubMed] [Google Scholar]
- Volk W. A. Cell wall lipopolysaccharides from xanthomonas species. J Bacteriol. 1966 Jan;91(1):39–42. doi: 10.1128/jb.91.1.39-42.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
- WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]
- WHEAT R. W., ROLLINS E. L., LEATHERWOOD J. M., BARNES R. L. Studies on the cell wall of Chromobacterium violaceum: the separation of lipopolysaccharide and mucopeptide by phenol extraction of whole cells. J Biol Chem. 1963 Jan;238:26–29. [PubMed] [Google Scholar]
