Abstract
The utilization of 1-alkenes by Micrococcus cerificans was investigated with respect to characteristic fatty acid profiles resulting from growth at the expense of these substrates. Saturated fatty acids containing even numbers of carbon atoms were produced from 1-dodecene and 1-tetradecene. Unsaturated fatty acids related to the parent alkene were not detected. The fatty acid profile from 1-pentadecene utilization resulted in the identification of 14-pentadecenoic acid, indicating preferential methyl-group attack. Studies with 1-hexadecene and 1-octadecene indicated simultaneous methyl-group and double-bond attack. Ω-Unsaturated fatty acids related to carbon number of parent alkene and odd-carbon fatty acids one carbon less than the substrate molecule were identified. A mechanism involving double bond epoxidation and oxidative cleavage was supported by measuring the release of formaldehyde. It appears that a dichotomous mechanism is functional in the assimilation of higher carbon number alkenes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HUYBREGTSE R., VANDERLINDEN A. C. THE OXIDATION OF ALPHA-OLEFINS BY A PSEUDOMONAS. REACTIONS INVOLVING THE DOUBLE BOND. Antonie Van Leeuwenhoek. 1964;30:185–196. doi: 10.1007/BF02046725. [DOI] [PubMed] [Google Scholar]
- Klug M. J., Markovetz A. J. Degradation of hydrocarbons by members of the genus Candida. II. Oxidation of n-alkanes and l-alkenes by Candida lipolytica. J Bacteriol. 1967 Jun;93(6):1847–1852. doi: 10.1128/jb.93.6.1847-1852.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Makula R., Finnerty W. R. Microbial assimilation of hydrocarbons. I. Fatty acids derived from normal alkanes. J Bacteriol. 1968 Jun;95(6):2102–2107. doi: 10.1128/jb.95.6.2102-2107.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Markovetz A. J., Klug M. J., Forney F. W. Oxidation of 1-tetradecene by Pseudomonas aeruginosa. J Bacteriol. 1967 Apr;93(4):1289–1293. doi: 10.1128/jb.93.4.1289-1293.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart J. E., Finnerty W. R., Kallio R. E., Stevenson D. P. Esters from Bacterial Oxidation of Olefins. Science. 1960 Oct 28;132(3435):1254–1254. doi: 10.1126/science.132.3435.1254. [DOI] [PubMed] [Google Scholar]