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. 1968 Jun;95(6):2151–2157. doi: 10.1128/jb.95.6.2151-2157.1968

Respiratory Pigments of Crithidia fasciculata

George C Hill 1, David C White 1
PMCID: PMC315147  PMID: 5669893

Abstract

Mitochondria were isolated from the heme-requiring insect trypanosomatid, Crithidia fasciculata, which had respiratory activity, showed a P/O ratio with succinate of 0.5 to 1.0, and contained 40 to 50% of the heme a and heme c found in the intact cells. Cytochromes b, c555, possibly c1, cytochrome oxidase, a carbon monoxide-binding pigment, and flavoproteins were detectable in the spectra of both intact cells and mitochondria. Cytochrome c555 is a basic protein that was extracted from cells and mitochondria with salt solutions. The molar ratio of heme c to heme a was approximately 2:1 in both cells and mitochondria. This organism could possibly serve as a model for studies of the respiratory activity of the pathogenic trypanosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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