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. 1968 Jun;95(6):2317–2324. doi: 10.1128/jb.95.6.2317-2324.1968

Vitamin K Deficiency in Fusiformis nigrescens I. Influence on Whole Cells and Cell Envelope Characteristics

Meir Lev 1
PMCID: PMC315167  PMID: 5669903

Abstract

Vitamin K depletion in the vitamin K-requiring anaerobe Fusiformis nigrescens resulted in the formation of elongated cells. In the presence of vitamin K, inhibition by cyanide and heme depletion did not affect cell morphology. Peptide depletion, as well as inhibition by chloramphenicol, ethionine, or fluorophenylalanine, also resulted in formation of elongated cells. Chemical analysis of cells and envelopes showed that protein, lipid, and deoxyribonucleic acid levels were similar in vitamin-supplemented and depleted cells; however, ribonucleic acid (RNA) levels in depleted cells and envelopes were significantly higher than in K-supplemented cells. Sections of cells of both groups showed minor differences in cell surfaces. The relationship between altered cell envelopes and increased RNA levels in K-deficient bacteria indicated a membrane-mediated effect of vitamin K on RNA and possibly protein synthesis in this microorganism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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