Abstract
We investigated the effect of the gas environment on the enzymatic reactions of intact isolated cells of the agents of trachoma and of meningopneumonitis of the host-dependent genus Chlamydia. In comparison with the reactions taking place in a gas phase of air, O2 depressed CO2 production from pyruvate and glutamate by trachoma and from glutamate by meningopneumonitis. O2 enhanced the degradation of pyruvate by meningopneumonitis, but this effect was due to increased H2O2, and was reversed by added catalase. Both dehydrogenation of α-ketoglutarate and was reversed by added catalase. Dehydrogenation of α-ketoglutarate by both agents and production of CO2 from C1 of glucose-6-phosphate were stimulated by O2 and depressed in N2. The latter activity was stimulated in air, O2, and N2 by nicotinamide adenine dinucleotide phosphate (NADP) in relation to the amount added, and also in air or O2, but not in N2, by moderate amounts of NADP and an excess of oxidized glutathione with concomitant formation of H2O2. A small but significant amount of O2 was consumed during the course of these reactions. It is suggested that glutathione reductase activity can occur only when accompanied by an oxidative reaction, and that this close link between the two reactions represents a mechanism of electron transport which transfers hydrogen to molecular O2.
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- COHEN G., HOCHSTEIN P. GLUTATHIONE PEROXIDASE: THE PRIMARY AGENT FOR THE ELIMINATION OF HYDROGEN PEROXIDE IN ERYTHROCYTES. Biochemistry. 1963 Nov-Dec;2:1420–1428. doi: 10.1021/bi00906a038. [DOI] [PubMed] [Google Scholar]
- KEILIN D., HARTREE E. F. Catalase, peroxidase and metmyoglobin as catalysts of coupled peroxidatic reactions. Biochem J. 1955 Jun;60(2):310–325. doi: 10.1042/bj0600310. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAPSON L. W., MOUSTAFA E. M. Ascorbic acid and glutathione as respiratory carriers in the respiration of pea seedlings. Biochem J. 1956 Feb;62(2):248–259. doi: 10.1042/bj0620248. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILLS G. C. Hemoglobin catabolism. I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown. J Biol Chem. 1957 Nov;229(1):189–197. [PubMed] [Google Scholar]
- MOULDER J. W., GRISSO D. L., BRUBAKER R. R. ENZYMES OF GLUCOSE CATABOLISM IN A MEMBER OF THE PSITTACOSIS GROUP. J Bacteriol. 1965 Mar;89:810–812. doi: 10.1128/jb.89.3.810-812.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas J. J., Neptune E. M., Sudduth H. C. Toxic effects of oxygen at high pressure on the metabolism of d-glucose by dispersions of rat brain. Biochem J. 1963 Jul;88(1):31–45. doi: 10.1042/bj0880031. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vender J., Moulder J. W. Initial step in catabolism of glucose by the meningopneumonitis agent. J Bacteriol. 1967 Oct;94(4):867–869. doi: 10.1128/jb.94.4.867-869.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEISS E., MYERS W. F., DRESSLER H. R., CHUN-HOON H. GLUCOSE METABOLISM BY AGENTS OF THE PSITTACOSIS-TRACHOMA GROUP. Virology. 1964 Apr;22:551–562. doi: 10.1016/0042-6822(64)90076-5. [DOI] [PubMed] [Google Scholar]
- Weiss E. Adenosine Triphosphate and Other Requirements for the Utilization of Glucose by Agents of the Psittacosis-Trachoma Group. J Bacteriol. 1965 Jul;90(1):243–253. doi: 10.1128/jb.90.1.243-253.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss E. Comparative metabolism of Chlamydia with special reference to catabolic activities. Am J Ophthalmol. 1967 May;63(5 Suppl):1098+–1098+. doi: 10.1016/0002-9394(67)94088-3. [DOI] [PubMed] [Google Scholar]
- Weiss E. Transaminase activity and other enzymatic reactions involving pyruvate and glutamate in Chlamydia (psittacosis-trachoma group). J Bacteriol. 1967 Jan;93(1):177–184. doi: 10.1128/jb.93.1.177-184.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]