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. 1968 Nov;96(5):1623–1631. doi: 10.1128/jb.96.5.1623-1631.1968

Trehalose Phosphate Synthesis in Streptomyces hygroscopicus: Purification of Guanosine Diphosphate d-Glucose: d-Glucose-6-Phosphate 1-Glucosyl-Transferase

Alan D Elbein a,1
PMCID: PMC315220  PMID: 5726304

Abstract

Guanosine diphosphate d-glucose:d-glucose-6-phosphate 1-glucosyl-transferase was purified approximately 100-fold from extracts of Streptomyces hygroscopicus. The purified enzyme catalyzed the transfer of glucose from guanosine diphosphate-d-glucose to glucose-6-phosphate to form trehalose phosphate and guanosine diphosphate. The enzyme was specific for these two substrates and was stimulated by the addition of magnesium ions. The product was characterized as α-α-trehalose-6-phosphate by its physical and chemical properties. The enzyme was present in a large number of Streptomyces species, suggesting that this group of organisms synthesized trehalose phosphate in a unique manner. This enzyme was not detected in fungi, since these organisms utilized uridine diphosphate-d-glucose as the glucosyl donor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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