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. 1969 Jun;98(3):970–978. doi: 10.1128/jb.98.3.970-978.1969

Characteristics of a New Sterol-nonrequiring Mycoplasma

Joseph G Tully 1, Shmuel Razin 2
PMCID: PMC315283  PMID: 4892385

Abstract

Two Mycoplasma strains recovered from tissue culture environments were found to grow in complex media devoid of serum or serum fractions containing cholesterol and in a cholesterol-free synthetic medium. Neither strain was capable of synthesizing pigmented carotenoids, although these compounds are present in, and characteristic of, other sterol-nonrequiring mycoplasmas. Serological tests and an analysis of their cell protein patterns obtained by gel electrophoresis indicated that the isolates were similar to each other but distinct from other sterol-nonrequiring serotypes, Mycoplasma laidlawii and M. granularum, as well as from sterol-requiring species. The existence of Mycoplasma other than M. laidlawii and M. granularum without sterol requirements suggested the need for some taxonomic changes in this group of organisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARGAMAN M., RAZIN S. CHOLESTEROL AND CHOLESTEROL ESTERS IN MYCOPLASMA. J Gen Microbiol. 1965 Jan;38:153–160. doi: 10.1099/00221287-38-1-153. [DOI] [PubMed] [Google Scholar]
  2. CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
  3. Del Giudice R. A., Robillard N. F., Carski T. R. Immunofluorescence identification of Mycoplasma on agar by use of incident illumination. J Bacteriol. 1967 Apr;93(4):1205–1209. doi: 10.1128/jb.93.4.1205-1209.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edward D. G., Freundt E. A., Chanock R. M., Fabricant J., Hayflick L., Lemcke R. M., Rezin S., Somerson N. L., Wittler R. G. Recommendations on nomenclature of the order Mycoplasmatales. Science. 1967 Mar 31;155(3770):1694–1696. doi: 10.1126/science.155.3770.1694. [DOI] [PubMed] [Google Scholar]
  5. Friend C., Patuleia M. C., De Harven E. Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells. Natl Cancer Inst Monogr. 1966 Sep;22:505–522. [PubMed] [Google Scholar]
  6. Friend C., Patuleia M. C., Nelson J. B. Antibiotic effect of tylosin on a mycoplasma contaminant in a tissue culture leukemia cell line. Proc Soc Exp Biol Med. 1966 Apr;121(4):1009–1010. doi: 10.3181/00379727-121-30950. [DOI] [PubMed] [Google Scholar]
  7. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Morowitz H. J., Tourtellotte M. E., Pollack M. E. USE OF POROUS CELLULOSE ESTER MEMBRANES IN THE PRIMARY ISOLATION AND SIZE DETERMINATION OF PLEUROPNEUMONIA-LIKE ORGANISMS. J Bacteriol. 1963 Jan;85(1):134–136. doi: 10.1128/jb.85.1.134-136.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RAZIN S., COHEN A. Nutritional requirements and metabolism of Mycoplasma laidlawii. J Gen Microbiol. 1963 Jan;30:141–154. doi: 10.1099/00221287-30-1-141. [DOI] [PubMed] [Google Scholar]
  11. RAZIN S. OSMOTIC LYSIS OF MYCOPLASMA. J Gen Microbiol. 1963 Dec;33:471–475. doi: 10.1099/00221287-33-3-471. [DOI] [PubMed] [Google Scholar]
  12. ROTHBLAT G. H., SMITH P. F. Nonsaponifiable lipids of representative pleuropneumonia-like organisms. J Bacteriol. 1961 Oct;82:479–491. doi: 10.1128/jb.82.4.479-491.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Razin S., Cleverdon R. C. Carotenoids and cholesterol in membranes of Mycoplasma laidlawii. J Gen Microbiol. 1965 Dec;41(3):409–415. doi: 10.1099/00221287-41-3-409. [DOI] [PubMed] [Google Scholar]
  14. Razin S. Mycoplasma taxonomy studiedy electrophoresis of cell proteins. J Bacteriol. 1968 Sep;96(3):687–694. doi: 10.1128/jb.96.3.687-694.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Razin S., Rottem S. Role of carotenoids and cholesterol in the growth of Mycoplasma laidlawii. J Bacteriol. 1967 Mar;93(3):1181–1182. doi: 10.1128/jb.93.3.1181-1182.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Razin S. The cell membrane of mycoplasma. Ann N Y Acad Sci. 1967 Jul 28;143(1):115–129. doi: 10.1111/j.1749-6632.1967.tb27651.x. [DOI] [PubMed] [Google Scholar]
  17. Razin S., Tourtellotte M. E., McElhaney R. N., Pollack J. D. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J Bacteriol. 1966 Feb;91(2):609–616. doi: 10.1128/jb.91.2.609-616.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rottem S., Gottfried L., Razin S. Carotenoids as protectors against photodynamic inactivation of the adenosine triphosphatase of Mycoplasma laidlawii membranes. Biochem J. 1968 Oct;109(4):707–708. doi: 10.1042/bj1090707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SMITH P. F. THE CAROTENOID PIGMENTS OF MYCOPLASMA. J Gen Microbiol. 1963 Sep;32:307–319. doi: 10.1099/00221287-32-3-307. [DOI] [PubMed] [Google Scholar]
  20. Smith P. F., Henrikson C. V. Growth inhibition of Mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol. J Bacteriol. 1966 May;91(5):1854–1858. doi: 10.1128/jb.91.5.1854-1858.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TOURTELLOTTE M. E., MOROWITZ H. J., KASIMER P. DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII. J Bacteriol. 1964 Jul;88:11–15. doi: 10.1128/jb.88.1.11-15.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. TULLY J. G. BIOCHEMICAL, MORPHOLOGICAL, AND SEROLOGICAL CHARACTERIZATION OF MYCOPLASMA OF MURINE ORIGIN. J Infect Dis. 1965 Apr;115:171–185. doi: 10.1093/infdis/115.2.171. [DOI] [PubMed] [Google Scholar]
  23. Tully J. G., Razin S. Physiological and serological comparisons among strains of Mycoplasma granularum and Mycoplasma laidlawii. J Bacteriol. 1968 May;95(5):1504–1512. doi: 10.1128/jb.95.5.1504-1512.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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