Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Jun;98(3):1026–1029. doi: 10.1128/jb.98.3.1026-1029.1969

Effect of Isomeric cis-Octadecenoic Acids on the Growth of Leptospira interrogans Serotype patoc

H M Jenkin 1,2, L E Anderson 1,2, R T Holman 1,2, I A Ismail 1,2, F D Gunstone 1,2
PMCID: PMC315290  PMID: 5788693

Abstract

Leptospira interrogans serotype patoc exhibited an increasing growth response when cultivated in media containing from 50 to 250 μg of sodium oleate per ml. Leptospiral growth in the presence of 250 μg of sodium oleate per ml was as good as that in the basal medium which contained 700 μg of oleic acid (in Tween 80) per ml. When positional isomers of oleic acid (9-octadecenoic acid) were present at a concentration of 200 μg/ml, the 2- and 8-isomers were not readily utilized, whereas the 3-, 4-, 6-, 11-, 15-, and 16-isomers gave a growth response equivalent to that of oleic acid, i.e., the 9-isomer. The 5-, 7-, 10-, 12-, 13-, 14-, and 17-isomers of octadecenoic acid induced growth responses which differed in magnitude but were intermediate to those of 2-18:1 and 3-18:1. When 200 μg of either 2- or 3-octadecenoic acid per ml was added in addition to 200 μg of 9-18:1 alone; 400 μg of 9-18:1 alone per ml inhibited growth of this organism. The growth response of leptospira to octadecenoic acids differed from that of mammalian cells, suggesting the presence of different enzymes in the two systems for the utilization of these substrates.

Full text

PDF
1026

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey J. M., Menter J. Lipid metabolism in cultured cells VII. Linoleic acid content of cells grown on lipid-free synthetic medium. Proc Soc Exp Biol Med. 1967 May;125(1):101–105. doi: 10.3181/00379727-125-32024. [DOI] [PubMed] [Google Scholar]
  2. ELLINGHAUSEN H. C., Jr, MCCULLOUGH W. G. NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: A SERUM-FREE MEDIUM EMPLOYING OLEIC ALBUMIN COMPLEX. Am J Vet Res. 1965 Jan;26:39–44. [PubMed] [Google Scholar]
  3. ELLINGHAUSEN H. C., Jr, MCCULLOUGH W. G. NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: FRACTIONATION OF OLEIC ALBUMIN COMPLEX AND A MEDIUM OF BOVINE ALBUMIN AND POLYSORBATE 80. Am J Vet Res. 1965 Jan;26:45–51. [PubMed] [Google Scholar]
  4. HELPRIN J. J., HIATT C. W. The effect of fatty acids on the respiration of Leptospira icterohemorrhagiae. J Infect Dis. 1957 Mar-Apr;100(2):136–140. doi: 10.1093/infdis/100.2.136. [DOI] [PubMed] [Google Scholar]
  5. JOHNSON R. C., GARY N. D. NUTRITION OF LEPTOSPIRA POMONA. II. FATTY ACID REQUIREMENTS. J Bacteriol. 1963 May;85:976–982. doi: 10.1128/jb.85.5.976-982.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JOHNSON R. C., GARY N. D. Nutrition of Leptospira pomona. 1. A chemically defined substitute for rabbit serum ultrafiltrate. J Bacteriol. 1962 Mar;83:668–672. doi: 10.1128/jb.83.3.668-672.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson R. C., Harris V. G. Differentiation of pathogenic and saprophytic letospires. I. Growth at low temperatures. J Bacteriol. 1967 Jul;94(1):27–31. doi: 10.1128/jb.94.1.27-31.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Phibbs P. V., Jr, Vaneseltine W. P. Nutritional requirements of Leptospirae. 3. Minimal nitrogen requirements of a strain of Leptospira pomona. J Infect Dis. 1968 Apr;118(2):197–205. doi: 10.1093/infdis/118.2.197. [DOI] [PubMed] [Google Scholar]
  9. Rose G. W., Eveland W. C., Ellinghausen H. C. Mechanisms of tissue cell penetration by Leptospira pomona: phagocytosis of leptospires in vitro. Am J Vet Res. 1966 Mar;27(117):503–511. [PubMed] [Google Scholar]
  10. STALHEIM O. H., WILSON J. B. CULTIVATION OF LEPTOSPIRAE. I. NUTRITION OF LEPTOSPIRA CANICOLA. J Bacteriol. 1964 Jul;88:48–54. doi: 10.1128/jb.88.1.48-54.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shenberg E. Growth of pathogenic Leptospira in chemically defined media. J Bacteriol. 1967 May;93(5):1598–1606. doi: 10.1128/jb.93.5.1598-1606.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. VANESELTINE W. P., STAPLES S. A. Nutritional requirements of Leptospirae. I. Studies of Leptospira pomona. J Infect Dis. 1961 May-Jun;108:262–269. doi: 10.1093/infdis/108.3.262. [DOI] [PubMed] [Google Scholar]
  13. VOGEL H., HUTNER S. H. Growth of leptospira in defined media. J Gen Microbiol. 1961 Oct;26:223–230. doi: 10.1099/00221287-26-2-223. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES