Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Jun;98(3):1080–1086. doi: 10.1128/jb.98.3.1080-1086.1969

Unique Protein Moieties for 30S and 50S Ribosomes of Escherichia coli

Bruce C Strnad 1, Paul S Sypherd 1
PMCID: PMC315299  PMID: 4892365

Abstract

Each major component of the proteins of 30S ribosomes from Escherichia coli was compared with the proteins of 50S ribosomes. The comparisons were done by using polyacrylamide gel electrophoresis in urea with differentially labeled proteins. The data show that no major protein is common to both ribosomes.

Full text

PDF
1080

Images in this article

1081Image
on p.1081
1084Image
on p.1084
1084Image
on p.1084
1084Image
on p.1084
1085Image
on p.1085
1085Image
on p.1085
1085Image
on p.1085

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brownlee G. G., Sanger F., Barrell B. G. The sequence of 5 s ribosomal ribonucleic acid. J Mol Biol. 1968 Jun 28;34(3):379–412. doi: 10.1016/0022-2836(68)90168-x. [DOI] [PubMed] [Google Scholar]
  2. CONWAY T. W., LIPMANN F. CHARACTERIZATION OF A RIBOSOME-LINKED GUANOSINE TRIPHOSPHATASE IN ESCHERICHIA COLI EXTRACTS. Proc Natl Acad Sci U S A. 1964 Dec;52:1462–1469. doi: 10.1073/pnas.52.6.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Capecchi M. R. Polypeptide chain termination in vitro: isolation of a release factor. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1144–1151. doi: 10.1073/pnas.58.3.1144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Fellner P., Sanger F. Sequence analysis of specific areas of the 16S and 23S ribosomal RNAs. Nature. 1968 Jul 20;219(5151):236–238. doi: 10.1038/219236a0. [DOI] [PubMed] [Google Scholar]
  6. Fogel S., Sypherd P. S. Chemical basis for heterogeneity of ribosomal proteins. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1329–1336. doi: 10.1073/pnas.59.4.1329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fogel S., Sypherd P. S. Extraction and isolation of individual ribosomal proteins from Escherichia coli. J Bacteriol. 1968 Aug;96(2):358–364. doi: 10.1128/jb.96.2.358-364.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gesteland R. F. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966 Mar;16(1):67–84. doi: 10.1016/s0022-2836(66)80263-2. [DOI] [PubMed] [Google Scholar]
  9. Hosokawa K., Fujimura R. K., Nomura M. Reconstitution of functionally active ribosomes from inactive subparticles and proteins. Proc Natl Acad Sci U S A. 1966 Jan;55(1):198–204. doi: 10.1073/pnas.55.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Monro R. E. Catalysis of peptide bond formation by 50 S ribosomal subunits from Escherichia coli. J Mol Biol. 1967 May 28;26(1):147–151. doi: 10.1016/0022-2836(67)90271-9. [DOI] [PubMed] [Google Scholar]
  11. Moore P. B., Traut R. R., Noller H., Pearson P., Delius H. Ribosomal proteins of Escherichia coli. II. Proteins from the 30 s subunit. J Mol Biol. 1968 Feb 14;31(3):441–461. doi: 10.1016/0022-2836(68)90420-8. [DOI] [PubMed] [Google Scholar]
  12. Stanley W. M., Jr, Salas M., Wahba A. J., Ochoa S. Translation of the genetic message: factors involved in the initiation of protein synthesis. Proc Natl Acad Sci U S A. 1966 Jul;56(1):290–295. doi: 10.1073/pnas.56.1.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sypherd P. S., Fansler B. S. Structural transitions in ribonucleic acid during ribosome development. J Bacteriol. 1967 Mar;93(3):920–929. doi: 10.1128/jb.93.3.920-929.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sypherd P. S. Ribosome development and the methylation of ribosomal ribonucleic acid. J Bacteriol. 1968 May;95(5):1844–1850. doi: 10.1128/jb.95.5.1844-1850.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sypherd P. S., Strauss N. CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963 Mar;49(3):400–407. doi: 10.1073/pnas.49.3.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Traub P., Hosokawa K., Craven G. R., Nomura M. Structure and function of E. coli ribosomes, IV. Isolation and characterization of functionally active ribosomal proteins. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2430–2436. doi: 10.1073/pnas.58.6.2430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Traub P., Nomura M. Structure and function of Escherichia coli ribosomes. I. Partial fractionation of the functionally active ribosomal proteins and reconstitution of artificial subribosomal particles. J Mol Biol. 1968 Jun 28;34(3):575–593. doi: 10.1016/0022-2836(68)90182-4. [DOI] [PubMed] [Google Scholar]
  18. Traub P., Söll D., Nomura M. Structure and function of Escherichia coli ribosomes. II. Translational fidelity and efficiency in protein synthesis of a protein-deficient subribosomal particle. J Mol Biol. 1968 Jun 28;34(3):595–608. doi: 10.1016/0022-2836(68)90183-6. [DOI] [PubMed] [Google Scholar]
  19. Traut R. R., Moore P. B., Delius H., Noller H., Tissières A. Ribosomal proteins of Escherichia coli. I. Demonstration of different primary structures. Proc Natl Acad Sci U S A. 1967 May;57(5):1294–1301. doi: 10.1073/pnas.57.5.1294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WILLIAMS D. E., REISFELD R. A. DISC ELECTROPHORESIS IN POLYACRYLAMIDE GELS: EXTENSION TO NEW CONDITIONS OF PH AND BUFFER. Ann N Y Acad Sci. 1964 Dec 28;121:373–381. doi: 10.1111/j.1749-6632.1964.tb14210.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES