Skip to main content
Journal of Bone and Mineral Research logoLink to Journal of Bone and Mineral Research
. 2010 Feb 23;25(8):1808–1820. doi: 10.1002/jbmr.72

Mouse BMD Quantitative Trait Loci Show Improved Concordance With Human Genome-wide Association Loci When Recalculated on a New, Common Mouse Genetic Map

Cheryl L Ackert-Bicknell 1, David Karasik 2, Qian Li 1, Randy V Smith 1, Yi-Hsiang Hsu 2, Gary A Churchill 1, Beverly J Paigen 1, Shirng-Wern Tsaih 1
PMCID: PMC3153351  PMID: 20200990

Abstract

Bone mineral density (BMD) is a heritable trait, and in mice, over 100 quantitative trait loci (QTLs) have been reported, but candidate genes have been identified for only a small percentage. Persistent errors in the mouse genetic map have negatively affected QTL localization, spurring the development of a new, corrected map. In this study, QTLs for BMD were remapped in 11 archival mouse data sets using this new genetic map. Since these QTLs all were mapped in a comparable way, direct comparisons of QTLs for concordance would be valid. We then compared human genome-wide association study (GWAS) BMD loci with the mouse QTLs. We found that 26 of the 28 human GWAS loci examined were located within the confidence interval of a mouse QTL. Furthermore, 14 of the GWAS loci mapped to within 3 cM of a mouse QTL peak. Lastly, we demonstrated that these newly remapped mouse QTLs can substantiate a candidate gene for a human GWAS locus, for which the peak single-nucleotide polymorphism (SNP) fell in an intergenic region. Specifically, we suggest that MEF2C (human chromosome 5, mouse chromosome 13) should be considered a candidate gene for the genetic regulation of BMD. In conclusion, use of the new mouse genetic map has improved the localization of mouse BMD QTLs, and these remapped QTLs show high concordance with human GWAS loci. We believe that this is an opportune time for a renewed effort by the genetics community to identify the causal variants regulating BMD using a synergistic mouse-human approach. © 2010 American Society for Bone and Mineral Research.

Keywords: genetic linkage, quantitative trait loci, mouse, human

Introduction

Currently, 43.7 million Americans over the age of 50 years are predicted to already have or be at serious risk of developing osteoporosis.(1) Bone mineral density (BMD) is a clinically measurable predictor of future fracture risk, and studies in humans have demonstrated that over 80% of the variance in peak bone mass is due to heritable factors.(2) Human genome-wide association studies (GWAS) have identified several significant loci for BMD, but collectively, the genome-wide significant (GWS) loci identified to date explain only a small portion of the variance in BMD.(3) This suggests that many more loci may be involved in the regulation of BMD.(4) Furthermore, identification of a locus by GWAS does not necessarily equal identification of the causal variant/gene.(5) For other complex phenotypes, such as high-density lipoprotein (HDL) cholesterol, it has been demonstrated that genetic studies in mice are very powerful tools for candidate gene identification in humans.(6) To date, well over 100 quantitative trait loci (QTLs) mapped in mice for BMD are reported in the literature, but few of the underlying genes have been identified.(7)

The QTLs found in single-cross analyses typically have confidence intervals (CIs) extending for 20 to 40 cM, encompassing hundreds of genes,(8) Two powerful techniques have been developed to combine information from multiple QTLs to narrow the list of candidate genes: combined cross-analysis and block haplotyping. Combined cross-analysis uses two or more crosses for which a QTL for the same phenotype has been mapped at the same or similar genetic location. The data from these crosses are merged, and the QTLs are remapped.(8) This technique improves both the power for QTL detection and QTL resolution.(9,10) Block haplotyping is the second method used to narrow QTLs. When a QTL is found, it is safe to assume that the gene responsible for the QTL will not be located where the two parental strains share a haplotype (ie, are genetically identical).(11) Instead, the gene will be located in a haplotype block for which the two strains are dissimilar. When a QTL has been identified in two distinct crosses at the same location, it therefore can be assumed that the gene will be in a region where the two parental strains carrying the high allele share a haplotype, where the two parental strains carrying the low allele share a haplotype, and where the high-allele and low-allele haplotypes are different. This block haplotyping approach can be used to reduce the size of the QTL region by up to 90%.(9,11,12)

Both these techniques rely on knowing what other QTLs have been mapped for a given phenotype and exactly where those QTLs are. Unfortunately, obtaining complete and accurate information about bone QTLs mapped in mice is not as straightforward as a simple literature search. The type and level of information published about the QTLs have improved along with the mapping techniques. Often, suggestive QTLs (ie, QTLs for which the significance level was above background but did not reach a p < .05 significance) are not reported for fear of “cluttering the literature.” Suggestive QTLs, nonetheless, can be used for both block haplotyping and combined cross-analysis to increase the power of detection and narrow the QTL. Pertinent information for a given QTL may never have been reported. Specifically, CIs or allele effects, both of which are required for block haplotyping, are often missing. Also, the marker closest to the peak may have been reported instead of the location of the actual QTL peak location. Depending on the density of the markers used in actually genotyping the cross, the marker closest to the peak and the actual peak of the QTL could be several cM apart. The algorithm used for QTL mapping often varies widely among studies, and different covariates such as body weight may or may not have been incorporated into the model, making absolute comparison of a pair of QTLs difficult. The last factor hindering the comparison of QTLs found in different studies is the fact that there are problems with the standard mouse genetic map.

The current standard genetic map for the mouse is curated and maintained by the Mouse Genome Informatics (MGI) Group at the Jackson Laboratory (http://www.informatics.jax.org/).(13) This map is based largely on information obtained from two small mouse crosses.(14,15) The traditional genetic map is updated on an annual basis and has improved over time,(16) but it still retains historical errors related to marker order and chromosome assignment.(17) Mapping QTLs requires accurate genetic map information for both the relative order of markers and the distances between them. Recently, Shifman and colleagues published a new genetic map based on a large heterogeneous stock population.(18) A total of 7080 standard simple-sequence-length polymorphism (SSLP) markers were integrated to this single-nucleotide polymorphism (SNP)–based map, generating a corrected mouse genetic map.(17) This new map resolves inconsistencies between the physical and genetic maps and provides highly accurate genetic distances. A recent mapping study, in which the new and traditional genetic maps were compared, suggests that up to 20% of published QTLs may have been mislocalized owing to marker order and positioning errors in the old genetic map.(17) In this study we have collected the raw data from the majority of the original mouse crosses for bone phenotypes. The QTLs for the BMD phenotypes measured in each cross were remapped using the new mouse genetic map. Lastly, the mouse QTL peak locations were compared with human BMD GWAS loci.

Materials and Methods

Identification of mapping-cross data sets

First, a literature search to identify published reports of mouse BMD QTLs was done using the following key words: BMD, mouse, QTL, bone mineral density, mapping, and/or loci. Second, the Mouse Genome Informatics Database (http://www.informatics.jax.org/) was searched for bone-related QTLs using the “Genes and Markers Query” form. Specifically, this database was searched using the keyword bone in the “Gene/Marker Symbol/Name” field, QTL in the “Type” field, and Any in the “Chromosome” field; the “No Limit” box was checked under the heading of “Maximum Returned.” QTLs for non-BMD phenotypes, such as “bone marrow graft rejection” and “bone area,” were struck from the returned list. The cross in which each BMD QTL was mapped was identified and compared with the list of crosses identified by conventional literature search. In short, the result was a list of mouse mapping crosses in which BMD QTLs were mapped regardless of the method used to measure density.

Main-effects QTL mapping

Map positions for the markers for all data sets were updated to the new mouse genetic map using a mouse map converter tool found at http://cdg.jax.org. All QTL analyses were done using the R/qtl software package(19) (R Version 2.6.2, qtl Library Version 1.09-43, http://www.rqtl.org/). Phenotypic outliers were removed prior to QTL analyses. In all cases, the phenotype trait data were transformed using the van der Waerden normal score method to correct for any skewing of the data.(20) Since we did not generate the original data, we performed rigorous quality-control analysis of the genotyping data prior to QTL mapping. First, errors in genotyping were identified by comparing the “User imputed marker positions” (ie, marker positions in cM from the new mouse genetic map) with the cross-calculated genetic position, as calculated in a cross-specific manner, using R/qtl. When the cross-calculated genetic positions were in large disagreement with marker positions input by the user, possible errors in marker ordering were examined. Second, the recombination fraction for all possible pairs of markers for a given cross were calculated using the est.rf function in R/qtl. The recombination fractions then were plotted using the plot.rf function in R/qtl. Markers exhibiting low recombination fraction/high LOD with nonadjacent markers (ie, markers at the opposite end of the same chromosome or with a marker on a completely different chromosome) were considered to be in error. When marker errors could not be resolved, the marker was deleted. A single-locus mainscan for QTL was performed, and LOD scores were calculated at 2-cM intervals across the genome using the “imp,” or imputation, method in R/qtl for all data sets except the B6xC3H data set from Farber and colleagues.(21) The data set from Farber and colleagues included genotyping for 1486 markers, with no missing genotypes. Because of this high density in genotyping data, marker regression was run for the main QTL scan for this one data set. The LOD thresholds for significant and suggestive QTLs were determined in a cross-specific manner based on 1000 permutations of the data.(22) A QTL was considered to be suggestive if the LOD score exceeded the p < .63 threshold and significant if it exceeded the p < .05 threshold. These thresholds were chosen because they are the widely accepted cutoffs for suggestive and significant QTLs.(23) The goal of this analysis was to identify main-effect QTLs; therefore, no pairwise/interactive genome scans were run.

For 5 of the 12 crosses, data were available for both male and female mice (Table 1). To account for the average differences between the males and females, we carried out scans using sex as an additive covariate. To identify sex-dependent QTL effects, we carried out additional scans using sex as an interactive covariate and computed the differences in LOD scores between these two scans (ie, the ΔLOD).(24) The interactive scan model identified the most likely position of the sex-specific QTLs. Calculating the ΔLOD score at the peak position is the secondary test for the QTL-by-sex interaction.(24) This secondary test is carried out with no adjustment for multiple testing, and the threshold, based on the usual chi-square distribution of the likelihood ratio, is 2.0 on the LOD scale. The sex specificity of these QTLs then was confirmed using the addqtl function in R/qtl by including “Qi:sex” in the main effected model, where i was the location of the putative sex-specific QTL. The cross generated by Farber and colleagues was bred in a reciprocal fashion (ie, B6 × C3H F1 × C3H × B6 F1), and therefore, cross-direction also was considered in the main effects model. Similar to the sex covariate, the strain of paternal grandmother (pgm) for each mouse was considered as both an additive and interactive covariate. The ΔLOD was calculated to identify potential “pgm-specific QTLs.” The covariate of “sex:pgm” also was included in the model to look for QTLs that were contingent on both cross-direction and gender. The pgm specificity of these QTLs was confirmed using the addqtl function, including “Qi:pgm” in the model, as was described for sex-specific QTLs.

Table 1.

Description of the Cross Data Sets

Cross (reference) Number of micea Phenotype(s) examined Age (weeks) Notesb
B6 × 129(35) 291 (F) Whole-body aBMD 20 Fed HF for last 14 weeks
Vertebral aBMD
B6 × C3H(34,54) 998 (F) Femoral vBMD 16
Vertebral vBMD
B6 × C3H(29) 145 (F) Femoral aBMD 35 Fed HF for last 14 weeks
164 (M)
B6 × CASA(52) 184 (F) Whole-body aBMD 11
185 (M)
B6 × CAST(36) 711 (F) Femoral vBMD 16 Selectively genotyped
B6 × DBA(5557) 595 (F) Whole-body aBMD 16 Data available for 2 ages, only used younger mice in this analysis
391 (M) Femoral aBMD
B6 × DBA(30) 110 (F) Femoral aBMD 68 Fed HF for last 16 weeks
Femoral vBMD
Midshaft vBMD
MRL × CAST(39) 170 (F) Whole-body aBMD 7
157 (M) Midshaft vBMD
MRL × SJL(32,33) 621 (F) Whole-body aBMD 7
Midshaft vBMD
NZB × RF(31) 661 (F) Femoral vBMD 10
Femoral cortical vBMD
Midshaft vBMD
Midshaft cortical vBMD
NZB × SM(58) 143 (F) Whole-body aBMD 24 Fed HF for last 16 weeks
124 (M) Vertebral aBMD
a

F = female; M = male.

b

HF = high-fat diet.

Comparison with human BMD genetic loci

Human BMD genome-wide significant loci from GWAS studies were identified from the literature.(3,2527) For each GWS locus, dbSNP (Build 130) was used to determine the genomic coordinates for the most significant SNP and 500 bp surrounding the SNP. These human genomic coordinates were lifted over to the mouse genome (Build 37) using the “Batch Coordinate Conversion” tool found at http://genome.ucsc.edu/index.html. If the Batch Coordinate Conversion lift-over was unsuccessful, the region surrounding the SNP was examined in the human genome browser window. The interval examined was expanded 1.5-fold until a conserved mouse/human sequence was identified on the “Mouse (July 2007/mm9) Alignment Net” track. The coordinates in the mouse genome for this conserved track were extracted. All mouse genomic coordinates then were converted to the cM position using the “Mouse Map Conversion Tool” found at http://cgd.jax.org/.

We wished to confirm that lifting over the genomic coordinates of the most significant SNPs from human to mouse meant moving over to the same gene “neighborhood” in both human and mouse. Therefore, we identified the mouse gene that was homologous to the human gene closest to the GWS peak. We then identified the chromosome, starting Mb and ending Mb, in the mouse for that gene. In all cases, we found that the coordinates for the peak SNP, as lifted over into mouse, were located in close proximity to the identified homologous mouse gene. This indicated that we were correctly moving the human GWAS peaks over to a similar gene neighborhood in the mouse.

Block haplotyping

Block haplotyping was done using the strain-comparison tool located at http://cgd.jax.org/. Specifically, the “Imputed Diversity Array, Build 37” SNP set was used. The following comparison expression was built:

graphic file with name jbmr0025-1808-m1.jpg (1)

All blocks consisting of at least one SNP extending for at least 1 bp were extracted. The results for all of chromosome 13 were downloaded, and any blocks outside our region of interest were deleted. The start and end positions for all mouse Refseq genes found in our region of interest were downloaded from the UCSC genome browser (http://genome.ucsc.edu). A gene was considered a candidate if a haplotype block was found anywhere between the genomic start and end positions for that gene. Since CASA/RkJ is a wild-derived strain, we were concerned that we were inappropriately removing genes from the candidate list by forcing SJL/J to equal CASA/RkJ in the haplotype block definition. We then repeated this exercise without the SJL/J equal to the CASA/RkJ term.

Results

Eligible data sets for reanalysis

Collectively, 15 mouse-mapping crosses in which BMD QTLs had been mapped were identified. The corresponding author and senior author for the first paper describing the data set were contacted and asked if they were willing to provide complete genotype and phenotype data for their data set for a mouse BMD QTL remapping project. Authors were asked separately if they were willing to deposit their data set into a public QTL data repository, such as the Center for Genome Dynamic's QTL archive (http://cgd.jax.org/nav/qtlarchive1.shtml). In total, 13 data sets of the 15 requested were received. No response was received from one set of authors, and one data set was not available for analysis. We decided not to include two data sets that were received for this project because they were too small and/or incomplete to do accurate mapping. The first of these two crosses(28) did not have genotype information available for every chromosome and had very low marker density (one to three markers per chromosome) for other chromosomes. The second of these(28) had only had 51 mice with genotype information available, making this data set underpowered for QTL detection in the algorithm we used. In sum, 11 data sets were used for this mouse BMD QTL recalculation project (Table 1).

Overview of QTLs mapped

In total, 155 primary QTLs were identified in the 11 data sets used for analysis, as is presented in Table 2: 86 QTLs for femoral BMD, 34 for vertebral BMD, and 35 for whole-body areal BMD (aBMD). At least one QTL for BMD was identified for each chromosome, with the exception of the Y chromosome (Fig. 1). We identified six QTLs that were specific to males, six QTLs that were specific to females, and 47 QTLs that were not sex specific. An additional 96 QTLs were mapped in crosses for which only data from female mice were available, and thus no determination of sex specificity could be made. One QTL, on chromosome 7, mapped in the B6 × C3H data set from Farber and colleagues,(29) was found to be cross-direction-specific, indicating a potential parent-of-origin effect.

Table 2.

Main Scan QTL Peaks Identified

Chr Peak (cM) LOD score Confidence interval (cM) Peak marker Phenotypea High allele Cross (reference) Gender specificb
1 51.66 2.13 1.66–97.30 D1Mit191 Femur aBMD B6 B6 × DB(56,57) F & M
1 62.50 27.50 58.50–64.50 D1Mit14 Femur vBMD C3H B6 × C3H(34) F (M ?)
1 63.84 3.23 34.46–94.35 D1Mit346 Whole-body aBMD MRL MRL × SJL(32,33) F (M ?)
1 69.01 3.41 48.81–89.51 rs3701299 Femur aBMD C3H B6 × C3H(29) F & M
1 72.50 14.04 66.50–76.50 D1Mit14 Vertebral vBMD C3H B6 × C3H(34) F (M ?)
1 74.46 2.32 46.46–92.46 D1Mit111 Femoral midcort vBMD NZB NZB × RF(31) F (M ?)
1 74.46 3.51 44.46–84.46 D1Mit111 Femoral mid vBMD NZB NZB × RF(31) F (M ?)
1 76.12 10.96 70.12–78.12 D1Mit15 Femur vBMD CAST B6 × CAST(36) F (M ?)
1 76.46 2.67 52.46–86.46 D1Mit111 Femur vBMD NZB NZB × RF(31) F (M ?)
1 76.73 4.06 70.08–94.08 D1Mit540.1 Whole-body aBMD CASA B6 × CASA(52) F & M
1 80.08 3.13 18.08–97.04 D1Mit115 Vertebral aBMD 129 B6 × 129(59) F (M ?)
1 87.66 4.49 79.66–91.66 D1Mit291 Whole-body aBMD DBA B6 × DBA(56,57) F & M
1 90.08 3.96 78.08–96.08 D1Mit406 Whole-body aBMD 129 B6 × 129(35) F (M ?)
1 90.46 5.87 82.46–94.35 D1Mit291 Femoral mid vBMD MRL MRL × SJL(32,33) F (M ?)
2 2.23 2.62 2.23–84.23 D2Mit1 Whole-body aBMD MRL MRL × CAST(39) F & M
2 3.62 3.64 3.62–14.54 rs3676722 Femur aBMD B6 B6 × C3H(29) M
2 40.90 4.12 30.90–46.90 D2Mit205 Whole-body aBMD B6 B6 × CASA(52) F & M
2 44.70 8.28 34.70–58.70 D2Mit91 Whole-body aBMD DBA B6 × DBA(56,57) F
2 57.81 4.32 47.81–62.49 D2Mit62 Whole-body aBMD MRL MRL × SJL(32,33) F (M ?)
2 60.23 1.75 24.23–94.23 D2Mit46 Femur vBMD B6 B6 × CAST(36) F (M ?)
2 69.80 2.72 62.70–102.29 D2Mit166 Femur aBMD DBA B6 × DBA(56,57) F
2 77.81 2.15 1.81–82.95 D2Mit285 Femoral mid vBMD SJL MRL × SJL(32,33) F (M ?)
2 79.42 4.04 73.42–88.99 D2Mit48 Femur vBMD C3H B6 × C3H(34) F (M ?)
2 85.81 2.29 51.81–100.49 D2Mit411 Femoral mid vBMD RF NZB × RF(31) F (M ?)
2 87.22 2.57 66.23–102.23 D2Mit413 Femur aBMD B6 B6 × DBA(30) F (M ?)
2 87.22 2.57 66.23–102.23 D2Mit413 Femur aBMD B6 B6 × DBA(30) F (M ?)
2 97.81 2.63 27.81–100.49 D2Mit148 Femoral midcort vBMD RF NZB × RF(31) F (M ?)
3 3.96 2.83 1.96–37.96 D3Mit23 Femur vBMD CAST B6 × CAST(36) F (M ?)
3 14.82 4.95 10.82–24.82 D3Mit203 Femoral mid vBMD RF NZB × RF(31) F (M ?)
3 34.85 2.55 3.55–51.21 rs3714671 Femur aBMD C3H B6 × C3H(29) F & M
3 38.19 2.74 24.01–62.01 D3Mit40 Femur aBMD B6 B6 × DBA(56,57) F & M
3 38.19 3.93 32.01–52.01 D3Mit40 Whole-body aBMD B6 B6 × DBA(56,57) F & M
3 68.85 4.00 54.85–72.85 D3Mit127 Femur vBMD DBA B6 × DBA(30) F (M ?)
4 44.43 2.96 26.43–56.43 D4Mit9 Femoral mid vBMD MRL MRL × CAST(39) F & M
4 48.04 15.07 45.76–54.04 D4Mit187 Vertebral vBMD C3H B6 × C3H(34) F (M ?)
4 52.04 22.94 50.04–66.04 D4Mit124 Femur vBMD C3H B6 × C3H(34) F (M ?)
4 62.14 2.82 48.14–66.26 D4Mit204 Femoral mid vBMD SJL MRL × SJL(32,33) F (M ?)
4 63.26 12.22 63.26–66.01 rs3023007 Femur aBMD C3H B6 × C3H(29) F & M
4 63.60 2.64 37.60–69.05 D4Mit251 Femur vBMD RF NZB × RF(31) F (M ?)
4 65.60 4.28 51.60–69.05 D4Mit251 Femoral cort vBMD RF NZB × RF(31) F (M ?)
4 68.43 3.04 54.43–82.64 D4Mit308 Whole-body aBMD 129 B6 × 129(35) F (M ?)
4 69.05 4.32 53.60–69.05 D4Mit251 Femoral midcort vBMD RF NZB × RF(31) F (M ?)
4 71.60 2.45 57.60–86.17 D4Mit68 Femur vBMD B6 B6 × CAST(36) F (M ?)
4 73.41 4.94 70.22–78.22 D4Mit48 Femur aBMD DBA B6 × DBA(56,57) F & M
4 73.41 10.94 70.22–76.22 D4Mit48 Whole-body aBMD DBA B6 × DBA(56,57) F & M
5 41.43 4.26 11.43–49.43 D5Mit10 Femoral mid vBMD MRL MRL × CAST(39) F
5 41.43 5.06 33.43–55.43 D5Mit112 Femur vBMD B6 B6 × CAST(36) F (M ?)
5 50.62 3.47 2.62–56.62 D5Mit10 Femoral midcort vBMD RF NZB × RF(31) F (M ?)
5 50.64 2.62 2.621–68.62 D5Mit10 Femoral mid vBMD RF NZB × RF(31) F (M ?)
5 77.43 2.44 3.43–86.57 D5Mit284 Vertebral aBMD SM NZB × SM(58) F & M
6 27.38 3.88 23.81–43.81 D6Mit384 Whole-body aBMD B6 B6 × CASA(52) F & M
6 29.81 4.03 25.40–57.81 D6Mit124 Femur vBMD B6 B6 × C3H(34) F (M ?)
6 31.81 3.61 17.81–41.81 D6Mit209 Vertebral aBMD 129 B6 × 129(35) F (M ?)
6 31.81 4.18 15.81–41.81 D6Mit209 Whole-body aBMD 129 B6 × 129(35) F (M ?)
6 52.15 4.41 26.15–66.15 D6MIT256 Whole-body aBMD MRL MRL × SJL(32,33) F (M ?)
6 53.81 3.95 49.81–73.81 D6Mit55 Femur aBMD B6 B6 × DBA(56,57) M
6 63.74 4.21 35.74–71.74 D6Mit25 Femur aBMD B6 B6 × DBA(30) F (M ?)
6 69.74 2.57 27.74–77.70 D6Mit198 Femur vBMD DBA B6 × DBA(30) F (M ?)
6 71.81 2.25 21.81–77.70 D6Mit15 Whole-body aBMD DBA B6 × DBA(56,57) F & M
6 75.81 1.97 1.81–77.70 D6Mit15 Vertebral vBMD B6 B6 × C3H(34) F (M ?)
6 77.64 2.08 15.81–77.64 D6Mit14 Femoral mid vBMD NZB NZB × RF(31) F (M ?)
7 13.94 7.53 11.94–25.94 D7Mit114 Femur aBMD DBA B6 × DBA(56,57) F & M
7 15.42 3.44 9.94–23.94 D7Mit114 Whole-body aBMD DBA B6 × DBA(56,57) F & M
7 16.94 2.93 8.83–43.97 rs4226499 Femur aBMD C3H B6 × C3H(29) F & M
7 20.71 2.21 2.71–31.44 D7Mit80 Femoral mid vBMD B6 B6 × DBA(30) F (M ?)
7 22.02 2.84 2.02–62.02 D7Mit310 Whole-body aBMD CASA B6 × CASA(52) F & M
7c 33.16 4.16 8.83–43.97 rs3688333 Femur aBMD C3H B6 × C3H(29) F & M
7 47.86 2.90 35.86–69.86 D7Mit300 Vertebral aBMD B6 B6 × 129(35) F (M ?)
7 69.46 4.84 47.46–77.87 D7Mit238 Vertebral vBMD B6 B6 × C3H(34) F (M ?)
7 74.71 4.52 54.71–84.71 D7Mit358 Femoral mid vBMD NZB NZB × RF(31) F (M ?)
7 76.71 5.16 68.71–82.71 D7Mit358 Femur vBMD NZB NZB × RF(31) F (M ?)
8 18.89 1.98 7.59–49.59 D8Mit4 Femur vBMD C3H B6 × C3H(34) F (M ?)
8 48.52 2.79 38.52–66.52 D8Mit211 Whole-body aBMD B6 B6 × CASA(52) F & M
8 50.75 3.81 20.75–58.75 D8Mit113 Femur aBMD DBA B6 × DBA(56,57) F & M
8 74.46 2.27 12.14–74.46 D8Mit42 Whole-body aBMD SM NZB × SM(58) F & M
9 32.76 2.68 12.46–44.46 D9Mit207 Whole-body aBMD MRL MRL × SJL(32,33) F (M ?)
9 35.80 2.24 23.80–47.80 D9Mit129 Femoral mid vBMD CAST MRL × CAST(39) F & M
9 42.32 3.82 36.46–53.48 D9MIT263 Femoral mid vBMD MRL MRL × SJL(32,33) F (M ?)
9 44.24 4.83 22.24–52.24 D9Mit196 Vertebral vBMD B6 B6 × C3H(34) F (M ?)
9 46.08 12.54 42.08–50.08 D9Mit196 Vertebral aBMD NZB NZB × SM(58) F
9 48.08 9.90 30.08–52.08 D9Mit196 Whole-body aBMD NZB NZB × SM(58) F
9 48.24 2.35 22.24–66.24 D9Mit196 Femur vBMD B6 B6 × C3H(34) F (M ?)
9 49.80 5.49 45.80–61.80 D9Mit10 Femur aBMD B6 B6 × DBA(56,57) F & M
9 63.8 2.34 19.80–71.49 D9Mit18 Femoral mid vBMD NZB NZB × RF(31) F (M ?)
9 66.48 2.95 42.48–71.32 D9Mit311 Whole-body aBMD B6 B6 × CASA(52) M
9 71.49 6.50 63.80–71.49 D9Mit18 Femur vBMD NZB NZB × RF(31) F (M ?)
10 2.53 2.58 2.53–58.53 D10Mit75 Whole-body aBMD B6 B6 × DBA(56,57) F & M
10 5.03 3.12 3.03–23.03 D10Mit28 Femur vBMD CAST B6 × CAST(36) F (M ?)
10 25.91 3.10 5.91–39.91 D10Mit31 Femoral mid vBMD CAST MRL × CAST(39) F & M
10 51.66 2.50 33.66–61.58 D10Mit95 Vertebral vBMD C3H B6 × C3H(34) F (M ?)
10 54.72 2.95 27.31–66.75 rs3672179 Femur aBMD B6 B6 × C3H(29) F & M
10 62.53 2.39 34.53–72.31 D10Mit162 Femur aBMD B6 B6 × DBA(56,57) M
10 66.75 2.30 7.27–66.75 D10Mit14 Femoral mid vBMD NZB NZB × RF(31) F (M ?)
11 33.04 6.20 24.20–37.81 rs4228731 Femur aBMD C3H B6 × C3H(29) M
11 34.70 6.81 26.70–46.70 D11Mit242 Femur vBMD C3H B6 × C3H(34) F (M ?)
11 38.70 7.19 26.70–42.88 D11Mit30 Femur vBMD RF NZB × RF(31) F (M ?)
11 44.7 8.49 36.70–60.70 D11Mit30 Femoral mid vBMD RF NZB × RF(31) F (M ?)
11 51.34 8.23 46.44–58.44 D11Mit355 Whole-body aBMD B6 B6 × DBA(56,57) F & M
11 60.70 2.82 4.70–74.70 D11Mit126 Femoral midcort vBMD RF NZB × RF(31) F (M ?)
11 70.70 2.92 34.70–75.93 D11Mit301 Vertebral vBMD C3H B6 × C3H(34) F (M ?)
12 2.94 4.60 2.94–24.94 D12Mit215 Femur vBMD B6 B6 × C3H(34) F (M ?)
12 5.52 2.91 5.52–33.52 D12Mit182 Femoral mid vBMD SJL MRL × SJL(32,33) F (M ?)
12 30.20 3.52 15.52–47.52 D12Mit201 Femur vBMD NZB NZB × RF(31) F (M ?)
12 33.52 6.32 25.52–57.52 D12Mit60 Whole-body aBMD NZB NZB × SM(58) F & M
12 43.52 5.24 31.52–69.52 D12Mit60 Vertebral aBMD NZB NZB × SM(58) F & M
12 60.56 2.60 2.94–60.56 D12Mit79 Vertebral vBMD C3H B6 × C3H(34) F (M ?)
13 5.08 3.33 3.08–51.11 D13Mit205 Femur vBMD CAST B6 × CAST(36) F (M ?)
13 6.05 2.18 6.05–54.05 D13Mit303 Whole-body aBMD DBA B6 × DBA(56,57) F & M
13 28.99 2.81 18.99–50.99 D13Mit13 Vertebral vBMD B6 B6 × C3H(34) F (M ?)
13 30.06 11.19 26.99–32.99 D13Mit13 Femur vBMD C3H B6 × C3H(34) F (M ?)
13 41.92 4.08 25.92–55.92 D13Mit202 Whole-body aBMD MRL MRL × SJL(32,33) F (M ?)
13 46.45 5.24 38.45–62.45 D13Mit191 Whole-body aBMD B6 B6 × CASA(52) F & M
13 57.92 2.24 15.92–65.43 D13Mit204 Femoral mid vBMD NZB NZB × RF(31) F (M ?)
13 65.17 2.57 30.31–67.21 rs3656262 Femur aBMD C3H B6 × C3H(29) F & M
14 8.03 2.55 6.03–59.53 D14Mit132 Femoral mid vBMD MRL MRL × SJL(32,33) F (M ?)
14 18.67 3.04 6.86–24.60 rs3709612 Femur aBMD C3H B6 × C3H(29) F & M
14 34.73 4.66 28.73–50.73 D14Mit160 Femur vBMD C3H B6 × C3H(34) F (M ?)
14 35.08 2.50 25.08–63.08 D14Mit142 Femur aBMD DBA B6 × DBA(56,57) F & M
14 42.73 4.58 32.73–55.83 D14Mit160 Vertebral vBMD C3H B6 × C3H(34) F (M ?)
14 56.16 3.66 31.53–63.53 D14Mit170 Femur vBMD CAST B6 × CAST(36) F (M ?)
14 57.08 2.05 13.08–59.53 D14MIT75 Vertebral aBMD 129 B6 × 129(35) F (M ?)
15 3.80 3.32 1.80–29.80 D15Mit13 Femur aBMD DBA B6 × DBA(30) F (M ?)
15 3.80 2.96 1.80–19.80 D15Mit13 Femur aBMD DBA B6 × DBA(30) F (M ?)
15 13.02 2.65 3.96–26.07 D15Mit111 Vertebral vBMD B6 B6 × C3H(34) F (M ?)
15 21.96 2.24 17.41–47.41 D15Mit26 Vertebral aBMD NZB NZB × SM(58) F & M
15 22.92 2.31 9.84–55.99 D15MIT115 Femoral mid vBMD MRL MRL × CAST(39) F & M
15 33.84 3.20 16.82–51.84 D15Mit63 Femur aBMD B6 B6 × DBA(56,57) F & M
15 34.16 4.54 29.80–47.80 D15Mit29 Femur vBMD B6 B6 × CAST(36) F (M ?)
15 35.84 3.34 22.92–49.84 D15MIT159 Whole-body aBMD MRL MRL × CAST(39) F & M
15 37.85 8.28 29.84–53.94 D15Mit2 Whole-body aBMD B6 B6 × CASA(52) F & M
15 38.67 3.88 25.84–47.84 D15Mit189 Whole-body aBMD B6 B6 × DBA(56,57) M
16 29.33 4.04 17.33–45.33 D16Mit12 Femur vBMD C3H B6 × C3H(34) F (M ?)
17 4.11 2.46 2.11–28.11 D17Mit164 Vertebral vBMD B6 B6 × C3H(34) F (M ?)
17 6.92 2.54 4.92–28.93 D17Mit143 Whole-body aBMD B6 B6 × DBA(56,57) F & M
17 16.93 4.16 4.92–34.92 D17Mit175 Femoral mid vBMD MRL MRL × SJL(32,33) F (M ?)
17 19.66 5.00 12.93–22.93 D17Mit176 Whole-body aBMD MRL MRL × SJL(32,33) F (M ?)
17 46.29 3.38 32.29–52.25 D17Mit39 Whole-body aBMD MRL MRL × CAST(39) F & M
17 52.11 3.12 42.11–55.02 D17Mit155 Femur vBMD C3H B6 × C3H(34) F (M ?)
17 55.93 2.27 43.93–60.67 D17Mit123 Femoral midcort vBMD NZB NZB × RF(31) F (M ?)
18 15.02 3.64 13.02–39.02 D18Mit34 Femoral mid vBMD MRL MRL × SJL(32,33) F (M ?)
18 24.64 8.11 20.46–28.46 D18Mit36 Vertebral vBMD C3H B6 × C3H(34) F (M ?)
18 28.46 17.19 22.46–34.46 D18Mit36 Femur vBMD C3H B6 × C3H(34) F (M ?)
18 40.14 2.83 21.02–43.02 D18MIT50 Vertebral aBMD 129 B6 × 129(35) F (M ?)
18 43.21 3.88 28.09–51.07 rs3669949 Femur aBMD C3H B6 × C3H(29) F & M
18 54.04 4.88 40.04–57.53 D18Mit4 Femoral midcort vBMD RF NZB × RF(31) F (M ?)
18 54.04 5.49 42.04–57.53 D18Mit4 Femoral midcort vBMD RF NZB × RF(31) F (M ?)
18 56.04 2.92 40.04–57.53 D18Mit4 Femur vBMD RF NZB × RF(31) F (M ?)
18 57.02 3.32 33.02–57.53 D18MIT43 Whole-body aBMD 129 B6 × 129(35) F (M ?)
18 57.53 1.75 14.53–57.53 D18Mit6 Femur vBMD CAST B6 × CAST(36) F (M ?)
19 11.04 1.58 3.04–43.04 D19Mit32 Femur vBMD CAST B6 × CAST(36) F (M ?)
19 12.34 3.24 4.34–28.34 D19Mit16 Femur aBMD DBA B6 × DBA(56,57) F
19 12.40 2.08 10.40–40.53 D19Mit61 Femur vBMD C3H B6 × C3H(34) F (M ?)
19 29.82 2.34 15.82–39.82 D19Mit11 Vertebral aBMD SM NZB × SM(58) F & M
19 43.33 2.46 23.33–56.28 D19Mit53 Femoral mid vBMD SJL MRL × SJL(32,33) F (M ?)
X 26.73 4.78 10.73–42.73 DXMit144 Femur aBMD B6 B6 × DBA(56,57) F & M
a

Midshaft (mid), cortical (cort).

b

Female (F), Male (M); no data available for males in that cross (?).

c

Cross-direction-specific, only seen in C3H × B6 cross direction.

Fig. 1.

Fig. 1

Mouse BMD QTLs compared with human GWAS loci. BMD QTLs for femoral (purple), whole-body (blue), and vertebral (green) BMD are presented to scale on the left side of each chromosome. Each white space along the chromosomal axis represents 10 cM. Each QTL is presented such that the vertical bar representing the QTL starts at the upper limit of the 95% confidence interval (CI) and extends to the lower end of the CI. The peak for the QTL is denoted by a black bar within the CI span. The human GWAS loci, as lifted over to the mouse genome coordinates, are represented as red triangles to the right of each chromosome.

For both the B6 × DBA cross generated by Drake and colleagues(30) and the NZB × RF cross,(31) multiple femoral BMD phenotypes were measured. In the B6 × DBA cross by Drake and colleagues, phenotypic data were available for femoral aBMD, femoral volumetric BMD (vBMD), and midshaft vBMD. In the NZB × RF data set, four phenotypes were measured: midshaft vBMD, midshaft cortical vBMD, total femoral vBMD, and total femoral cortical vBMD. In some instances, QTLs with the same peak location and similar CIs were found for more than one femoral phenotype within a single cross. Since there also were unique QTLs for each phenotype, we have presented all QTLs identified for BMD, regardless of type, mapped in these two data sets.

Comparison with human GWAS loci

To date, 20 substantiated (ie, p < 5 × 10−8) genome-wide significant (GWS) loci for BMD have been identified in a large meta-analysis of five independent GWAS (the GEFOS Consortium).(3) We compared the locations of these 20 loci with the mouse QTLs (Table 3). We found that 16 of the 20 most significant GWS markers fell within the CI of a mouse QTL. Two of the GWS loci that did not appear, at first examination, to fall within a mouse QTL were very interesting. The first of these, located on human chromosome 3 at 41 Mb, mouse chromosome 9 at 120.7 Mb (72 cM), is only 0.5 cM away from the peak of an acentric QTL mapped in NZB × RF (peak location of 71.49 cM). The second of these, located on human chromosome 6 at 152 Mb, mouse chromosome 10 at 5.7 Mb (2.1 cM), is near the peak of an acentric QTL, 0.4 cM away, mapped in B6 × DBA (peak location of 2.53 cM). In both these cases, the mouse QTL peak location is at or near the last/first marker genotyped in the cross for that chromosome. There are no other informative flanking markers by which to judge if the QTLs are truly acentric. Thus we consider these two human GWS loci likely to be within a mouse QTL. Nine of the human GWS loci are located with 2 cM of the peak location for a mouse QTL (Table 3). An additional four of these loci are within 5 cM of the peak of mouse QTL.

Table 3.

Comparison of Human GWAS Loci With Mouse QTLs

Human Mouse


Study (reference) SNP IDa Chr. Bp Chr. Bp cM In mouse QTL? Nearest mouse QTL (cM)
GEFOS(3) rs7524102 1 22,570,784 4 136,661,533 69.3 Yes 0.2
rs6426749 1 22,583,810 4 136,657,367 69.3 Yes 0.2
rs1430742 1 68,407,413 3 159,552,734 82.7 No
rs2566755 1 68,407,728 3 159,552,348 82.7 No
rs11898505 2 54,537,811 11 30,167,162 17.7 Yes 15.3
rs87938 3 41,112,426 9 120,766,377 72.0 Likely 1.0
rs1471403 4 88,994,017 5 104,773,742 50.7 Yes 0.1
rs1366594 5 88,411,567 13 83,453,756 43.7 Yes 1.8
rs2941740 6 152,051,081 10 5,736,893 2.1 Likely 1.0
rs2504063 6 152,132,150 10 5,667,438 2.1 Likely 1.0
rs1524058 7 38,102,552 13 19,538,734 6.9 Yes 0.9
rs4729260 7 95,955,604 6 6,305,527 2.5 Yes 24.9
rs7781370 7 95,971,217 6 6,323,120 2.5 Yes 24.9
rs2062377 8 120,076,351 15 54,166,754 21.1 Yes 0.8
rs11995824 8 120,081,631 15 54,179,867 21.1 Yes 0.8
rs7117858 11 15,650,788 7 122,379,000 60.9 Yes 8.6
rs16921914 11 31,167,097 2 105,937,807 55.5 Yes 2.3
rs7932354 11 46,678,547 2 91,490,042 50.6 Yes 5.9
rs599083 11 67,948,672 19 3,604,166 3.3 Yes 7.7
rs2016266 12 52,013,972 15 102,194,741 57.5 No
rs9533090 13 41,849,199 14 78,848,972 41.4 Yes 1.3
rs10048146 16 85,267,911 8 123,719,142 70.4 Yes 4.1
rs228769 17 39,548,461 11 102,084,442 66.0 Yes 4.7
rs9303521 17 41,160,727 11 103,944,446 67.7 Yes 3.0
rs884205 18 58,205,587 1 107,744,514 49.7 Yes 1.9
U.S. Caucasians(26,27) rs17131547 1 91,983,358 5 107,591,590 52.1 Yes 1.5
rs4276378 5 7,297,491 13 69251843 35.6 Yes 5.5
rs1823926 5 119,882,620 18 51320749 28.0 Yes 0.4
rs11239762 10 42,582,267 6 118362999 55.9 Yes 2.1
rs12437971 15 98,662,088 7 74,018,517 36.3 Yes 3.1
rs16945612 16 75,986,223 8 116,327,071 60.1 Yes 9.4
KoGES(25) rs7776725 7 120,820,107 6 22,304,412 9.3 Yes 18.1
rs550677 12 124,376,759 5 126,093,513 64.3 Yes 13.1
a

Some peaks in the GEFOS Study were denoted by two-peak SNPs. For consistency, both GEFOS peak SNPs are presented. Peaks in the U.S. Caucasians and KoGES studies that were within 1 Mb of peaks in the GEFOS Study are not included.

GWS loci for BMD also have been indentified in smaller cohorts independent of the GEFOS meta-analysis GWAS. While these other cohorts did identify some of the same loci as were found in the GEFOS study, eight additional GWS loci have been described. Deng and colleagues have identified six loci in a small cohort of U.S. Caucasians.(26,27) All six of these loci mapped within the CI of a mouse BMD QTL. Three of these six loci loci mapped to within 2 cM of the peak for a mouse QTL, and a fourth mapped to within 3.1 cM (Table 3). Two more BMD loci were identified in large study of Korean individuals (KoGES Study cohorts).(25) While both these GWS loci were found within the CIs of mouse BMD QTLs, neither of these loci mapped in close proximity to a mouse QTL peak. In the KoGES Study, BMD of the distal radius and of the midshaft of the tibia was measured,(26) not BMD of the femoral neck and lumbar spine, as was measured in the other two GWAS. Three of the KoGES loci were confirmed in the GEFOS Study (human chromosome 7, rs1721400, human chromosome 7, rs6974574, and human chromosome 13, rs9525667) and may represent loci controlling BMD “globally.” The other two KoGES loci may be more site-specific regulators of BMD, and thus it may not be straightforward to compare these loci with the other human loci and with the spinal, femoral, and whole-body BMD QTLs in the mouse.

Confirmation of Mef2c as a candidate gene for BMD

In the GEFOS Study, the most significant SNP at 5q14 for femoral BMD was located in an intergenic region approximately 200 kb distal to the MEF2C coding sequence. All the surrounding SNPs with a p value of less than 1 × 10−8 also were located in this intergenic region, and none of these SNPs is in strong linkage disequilibrium with SNPs in the coding region of MEF2C.(3) Furthermore, 5q14 is a novel GWS locus for BMD and has not been replicated thus far in an independent cohort. Thus we wished to substantiate MEF2C as a gene that should be further examined for genetic association with BMD. This GWS locus in mice is located between two QTL peaks for aBMD that had been mapped in MRL × SJL and B6 × CASA (Fig. 2). We used block haplotyping to determine if Mef2c could be a candidate for these two mouse QTLs. In short, 338 unique Refseq genes fall within the confidence interval for the MRL × SJL QTL, 186 in the B6 × CASA QTL, with 153 genes falling in the region wherein the CI for these two QTLs overlap. By block haplotyping, we reduced the list of possible candidate genes to six genes, including Mef2c (Fig. 2). We repeated the haplotyping without the SJL/J equal to the CASA/RkJ term and confirmed that Mef2c should be considered a candidate for these QTLs in mice.

Fig. 2.

Fig. 2

Confirmation of Mef2c as candidate gene for BMD on mouse chromosome 13. A GWS locus was identified on human chromosome 7 with a peak at rs1524058. The GWS locus is found in close proximity to two BMD QTLs in the mouse that were identified in MRL × SJL and B6 × CASA. The confidence intervals (CI) for these two mouse QTLs overlap for the interval from 38 to 55.9 cM (72.1 to 104 Mb). A total of 338 Refseq genes are located within the MRL × SJL CI, 186 genes within the B6 × CASA CI, and 153 genes are located where the CI for these two QTLs overlap. Using block haplotyping, we were able to eliminate all but six of these genes as candidates for this QTL. The gene Mef2c (black box) is the gene closest to the human GWS locus, and it remained a candidate after haplotyping.

Discussion

Impact of the new genetic map on studies based on congenic mice

A congenic is a strain of mice wherein the alleles for specific genetic regions from one inbred strain (the donor strain) are moved over to another inbred strain (the background strain) using a backcross-breeding scheme. Congenic mice are useful in that they can confirm that the alleles of the donor strain at that locus change the phenotype of interest relative to the background strain. Congenics are powerful tools to examine the basic biology underlying a QTL. Congenic mice are genotyped to ensure that the genetic region of interest has been carried over from the donor strain onto the background strain. It is possible to make a congenic region for the “wrong” genetic location (ie, somewhere other than for which the data suggest there is a QTL) and still get a phenotype. QTL analysis is designed to identify association between a genotype and phenotype that achieves some level of statistical significance. Not every locus is going to be identified in a given F2 cross, especially if that cross contained only a small number of animals. Congenic studies still in the planning stages could be affected by our finding in that the genetic location of the QTL has “moved.” However, as long as the investigator uses the same markers found to be closest to the QTL peak and the associated flanking markers used in the F2 analysis to make the congenic, the correct congenic piece from the donor strain will be carried over.

Concordant QTLs

One of the main purposes of our study was to provide a listing of BMD QTLs, all mapped using a common genetic map and a common mapping algorithm, such that QTLs from different crosses could be compared with ease and confidence. Furthermore, it has long been thought that there should be concordance in peak location for QTLs identified by different crosses. However, because of discrepancies in the genetic map and in the various calculation algorithms, grouping QTL peaks based on literature-reported peak location was at best tricky and at worse misleading. In this recalculation study, we were not hindered by these obstacles, and we therefore can group QTLs with increased confidence. Indeed, there are clear examples where QTLs identified by various groups may be one and the same. For example, on chromosome 18 we report 10 different QTLs identified in six different crosses (Table 2). Based on peak location, our data suggest that there may be only 4 unique QTLs, not 10. The first of these QTLs is supported only by a single cross, with a peak at 15 cM.(32,33) The second QTL is also supported by only a single cross, the B6 × C3H cross generated by Beamer and colleagues,(34) but the peaks for vertebral vBMD (at 25 cM) and for femoral vBMD (at 29 cM) are likely one QTL regulating global BMD. The peak location for this QTL maps to the same location as a GWAS peak identified by Deng and colleagues.(27) The third QTL group is supported by two different crosses and is represented by a QTL mapped in B6 × 129(35) at 40 cM and by a second QTL mapped in the B6 × C3H cross generated by Farber and colleagues,(29) with a peak at 43 cM. The fourth and final QTL is the most interesting in that it was identified in three crosses: B6 × CAST(36) at 58 cM, B6 × 129(35) at 56 cM, and for three bone phenotypes in NZB × RF(31) at peaks 54, 54, and 56 cM.

A traditional F2 cross has poor mapping resolution, so it is difficult to say with confidence how far apart two QTLs can be and still be caused by the same genetic entity. Furthermore, concordant peak location does not mean that the two QTLs share the same genetic cause. There are statistical tests such as biallelic combining of crosses(8) that can be done to interrogate whether two crosses share a QTL or if that QTL is cross-specific. Performing such analysis for all possible shared QTLs is beyond the scope of this study.

Differences among data sets

While this study does reconcile differences in mapping algorithms and genetic maps, fundamental differences in study design used by different investigators also should be considered. The mice examined in four of the crosses had been fed a high-fat diet prior to obtaining the phenotype data (Table 1). A high-fat diet may alter the BMD, and it is likely that gene-by-dietary-fat interaction(s) may affect BMD as well.(37) Earlier we discuss an example for chromosome 18. The second chromosome 18 QTL grouping is solely represented by B6 × C3H. The third bin also contains a B6 × C3H femoral BMD QTL, but in this case, the mice from the mapping cross had been fed a high-fat diet. In fact, both crosses in this third bin of QTLs had been fed a high-fat diet. Thus the use of a different diet likely suggests that there are indeed two distinct B6 × C3H BMD QTLs on chromosome 18 and that this finding is not a function of poor mapping resolution.

The age of the mice measured for BMD does differ among the 11 data sets analyzed. Peak BMD in mice is obtained at or around 16 weeks of age for most inbred strains.(38) For the majority of data sets, BMD was measured at or near to this age of peak BMD. It must be noted that the mice in the MRL × CAST(39) and MRL × SJL(40) crosses were substantially younger than 16 weeks of age, and for one of the two B6 × DBA crosses, the mice were much older (Table 1). This age difference may explain the differences in QTLs identified in the two B6 × DBA crosses.

For each data set, BMD was obtained using a slightly different protocol. In essence, BMD can be broken down into two types: volumetric BMD (vBMD), as obtained by a computed tomographic approach, and areal BMD (aBMD), for which the BMD was obtained by densitometric analysis of plain-film X-rays or by using a dual-energy X-ray absorptiometer. The BMD data also can be classed by anatomic site of measure: whole-body scans of the head, femur, or vertebra. We list the type of BMD phenotype data for each cross in Table 1. For presentation purposes, we have subdivided the QTLs based on the anatomic site at which BMD was obtained (Fig. 1), but we list the exact BMD phenotype for each QTL in Table 2. Two QTLs with concordant peak locations but for a different anatomic site may well be caused by the same genetic entity, as is suggested earlier by the femoral and vertebral BMD QTLs on chromosome 18 mapped in the B6 × C3H data set from Beamer and colleagues. Other studies, however, have suggested intrinsic differences in bone physiology at different anatomic sites.(41) Thus we have subdivided the QTLs by anatomic site.

Comparison with human BMD loci

Traditional QTLs for BMD have been mapped in humans in many small studies.(42) Meta-analyses of the human BMD QTLs have been done to improve the power to detect these QTLs.(43,44) In a meta-analysis of genetic loci, the genome is divided into equal-sized bins, and bins with significant linkage to the phenotype are reported. While a valid approach to overcome small-sample-size issues, meta-analysis loci cannot be lifted over easily from one species to another. By definition, the meta-analysis bins are large, and no specific peak location within that bin is calculated. Thus a single-human-genome bin may be homologous to multiple mouse chromosomal regions. For this reason, it is impractical to compare the mouse QTLs with the human BMD meta-analysis results, and we have therefore chosen to compare the mouse QTLs with the human loci mapped in GWAS. The human GWAS loci are sufficiently narrow that they can be lifted over to a single-mouse-chromosomal location, and the GWAS loci reach a genome-wide significant threshold.

There are limitations of the GWAS studies done to date in humans. First of all, no vBMD QTLs have been mapped; only aBMD data are available. Second, sex specificity of the QTLs has not been considered. Third, the X chromosome has not been considered in the GWAS studies for bone. Fourth, GWAS studies consider only the common SNPs (usually minor allele frequency > 1%).(4) Fifth, large GWAS studies for some ethic groups are lacking. Sixth, the highly significant human GWS loci explain only a fraction of the variance found in aBMD in humans.(3) In this study we describe 155 QTLs for mice. As stated earlier, it would be beyond the scope of this article to statistically assess how many of these loci are truly concordant. That said, we estimate that these 155 loci can be collapsed into about 85 distinct loci for BMD identified to date in the mouse. The GWAS studies provide us with a candidate gene(s) to test for only 30% of these QTLs. Finding the genes underlying the remaining 70% of the mouse loci and then testing those genes in humans could be one way of trying to capture some of the “missing variation” or so-called genetic dark matter endemic in GWAS studies.

Work with congenic strains has suggested that there are at least five distinct QTLs on mouse chromosome 1: 37 to 41 cM, 68 to 70 cM, 79 to 80 cM, at 84 cM, and 90 to 97 cM,(4548) in addition to the B6 × DBA peak at 51.7 cM for femoral aBMD. Only the B6 × DBA peak is likely explained by the GWAS loci identified to date. Edderkaoui and colleagues have determined that Duffy blood group, chemokine receptor (Darc), likely explains at least one of these QTL,(49) but this gene has not yet been examined for association with BMD in humans. Lipoxygenase 15 (Alox15) was identified as a QTL candidate gene for mouse chromosome 11. This gene was found to be associated with BMD in Chinese women,(50) an ethnic group not yet well represented by a large GWAS. These two examples demonstrate that the mouse can be used to find genes that regulate BMD outside the GWAS loci known to date.

While the resolution of mapping in a GWAS is far superior to the more traditional QTL-based mapping in humans, identification of a GWS locus does not necessary equal immediate identification of a causative gene.(5) Approximately half the human GWS loci for BMD fall in intergenic regions of the genome. Still more GWS loci fall in gene-dense regions, wherein there is more than one candidate gene for the locus.(3) By examining concordant QTLs in mice, we can better resolve these more enigmatic GWS loci to an actual gene or causal variant and make models for the study of the biologic mechanism by which the locus affect BMD. For example, the peak SNP and all the subsignificant supporting SNPs for the human chromosome 5 rs1366594 GWS locus are located in an intergenic region near to the human MEF2C gene.(3) Expression of Mef2c has been demonstrated in both osteoblasts and osteocytes in rodents, and Mef2c is thought to regulate Sost expression, proving a role for this gene in bone biology.(51) Using block haplotyping, we were able to confirm that this gene should be considered a candidate gene for this QTL. Moreover, a potential splice-site polymorphism has been identified in this gene in several strains of mice (rs47941354), which may be the underlying causal variant. Body weight and/or body size can influence BMD. Vitarius and colleagues, when originally analyzing the B6 × CASA cross, identified a body-weight QTL on chromosome 13.(52) It is possible that Mef2c affects BMD by altering body mass. QTLs for forelimb muscle mass and body length also were examined in the MRL × SJL cross, but no QTLs on chromosome 13 for these two phenotypes are reported.(53) This lack of a lean-mass QTL in the MRL × SJL cross does not rule out a possible role for Mef2c in the modulation of fat mass or total body weight to affect bone mass. This possible interaction between body weight and fat mass requires further study. Thus studies in mice can be used to confirm novel GWAS loci, can be used to identify actual causal polymorphisms, and can be used to establish the role of the candidate gene in bone biology.

Conclusion

In summary, we remapped the QTLs from 11 mouse mapping crosses using a single mapping study design and using a single corrected version of the mouse genetic map. Our results demonstrate that the BMD QTLs found in different crosses bin together in many instances across the genome, validating the use of such QTL narrowing techniques as combined cross-analysis and block haplotyping. We have shown that QTLs mapped with this new version of the mouse genetic map do agree very well with human BMD GWS loci. With the newly available high-resolution GWAS mapping in humans and our improved QTL mapping in mouse, we believe that this is an opportune time for a renewed effort by the genetics community to identify the causal variants regulating BMD using a synergistic mouse-human approach.

Acknowledgments

We would like to thank all the individuals and research groups who provided their mouse mapping cross data for this project. In particular, we would like to acknowledge the contributions of the following individuals: Dr W Beamer, Dr S Mohan, Dr J Wergedal, Dr C Farber, Dr T Drake, Dr E Schadt, Dr J Lusis, Dr J Vitarius, Dr J Breslow, Dr R Klein, Dr R J Shmookler Reis, and Dr E Orwoll.

We also would like to thank Mr J Hammer and Ms J Currer for their assistance preparing this manuscript and Dr T Gridley for critically reviewing it.

CLAB was funded by NIH Grants AG034019 and HD7065-30. DK and YHH were funded by NIH Grant AR053992. SWT was funded by Ellison Medical Foundation Grant AG-NS-0421-07. GAC was funded by NIH Grant GM070683. In addition, this project was supported by the Jackson Laboratory, Nathan Shock Center of Excellence in the Basic Biology of Aging, NIH Grant AG025707.

Disclosures

All the authors state that they have no conflicts of interest.

References

  • 1.US Department of Health and Human Services. Bone Health and Osteoporosis: A Report of the Surgeon General. 2004. Rockville, MD: US Department of Health and Human Services, Office of the Surgeon General.
  • 2.Ralston S, de Crombrugghe B. Genetic regulation of bone mass and susceptibility to osteoporosis. Genes Dev. 2006;20:2492–2506. doi: 10.1101/gad.1449506. [DOI] [PubMed] [Google Scholar]
  • 3.Rivadeneira F. Twenty bone-mineral-density loci identified by large-scale meta-analysis of genome-wide association studies. Nat Genet. 2009;151:528–537. doi: 10.1038/ng.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Ferrari S. Human genetics of osteoporosis. Best Pract Res Clin Endocrinol Metab. 2008;22:723–735. doi: 10.1016/j.beem.2008.08.007. [DOI] [PubMed] [Google Scholar]
  • 5.McCarthy M, Hirchhorn J. Genome-wide association studies: potential next steps on a genetic journey. Hum Mol Genet. 2008;17:R156–R165. doi: 10.1093/hmg/ddn289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Rollins J, Chen Y, Paigen B, Wang X. In search of new targets for plasma high-density lipoprotein cholesterol levels: promise of human-mouse comparative genomics. Trends Cardiovasc Med. 2006;16:220–234. doi: 10.1016/j.tcm.2006.04.003. [DOI] [PubMed] [Google Scholar]
  • 7.Ackert-Bicknell CL, Rosen CJ. The future of mouse genetics in osteoporosis research. BoneKEy-Osteovision. 2009;6:200–209. [Google Scholar]
  • 8.Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining Data From Multiple Inbred Line Crosses Improves the Power and Resolution of Quantitative Trait Loci Mapping. Genetics. 2005;169:1699–1709. doi: 10.1534/genetics.104.033993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Burgess-Herbert SL, Cox A, Tsaih S-W, Paigen B. Practical Applications of the Bioinformatics Toolbox for Narrowing Quantitative Trait Loci. Genetics. 2008;180:2227–2235. doi: 10.1534/genetics.108.090175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Su Z, Ishimori N, Chen Y, et al. Four additional mouse crosses improve the lipid QTL landscape and identify Lipg as a QTL gene. J. Lipid Res. 2009;50:2083–2094. doi: 10.1194/jlr.M900076-JLR200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Wang X, Paigen B. Genetic Variation in HDL Cholesterol in Humans and Mice. Circ Res. 2005;96:27–42. doi: 10.1161/01.RES.0000151332.39871.13. [DOI] [PubMed] [Google Scholar]
  • 12.DiPetrillo K, Wang X, Stylianou I, Paigen B. Bioinformatics toolbox for narrowing rodent quantitative trait loci. Trends Gen. 2005;21:683–692. doi: 10.1016/j.tig.2005.09.008. [DOI] [PubMed] [Google Scholar]
  • 13.Blake J, Richardson J, Davisson M, Eppig J. The Mouse Genome Database (MGD). A comprehensive public resource of genetic, phenotypic and genomic data. The Mouse Genome Informatics Group. Nucl. Acids Res. 1997;25:85–91. doi: 10.1093/nar/25.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Dietrich WF, Miller JC, Steen RG, et al. A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nat Genet. 1994;7:220–245. doi: 10.1038/ng0694supp-220. [DOI] [PubMed] [Google Scholar]
  • 15.Rowe L, Nadeau J, Turner R, et al. Maps from two interspecific backcross DNA panels available as a community genetic mapping resource. Mammalian Genome. 1994;5:253–274. doi: 10.1007/BF00389540. [DOI] [PubMed] [Google Scholar]
  • 16.Blake JA, Bult CJ, Eppig JT, Kadin JA, Richardson JE, the Mouse Genome Database Group The Mouse Genome Database genotypes::phenotypes. Nucl. Acids Res. 2009;37(suppl_1):D712–719. doi: 10.1093/nar/gkn886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Cox A, Ackert-Bicknell CL, Dumont B, et al. A new standard genetic map for the laboratory mouse. Genetics. 2009;182:1335–1344. doi: 10.1534/genetics.109.105486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Shifman S, Bell J, Copley R, et al. A High-Resolution Single Nucleotide Polymorphism Genetic Map of the Mouse Genome. PloS Biology. 2006;4:2227–2237. doi: 10.1371/journal.pbio.0040395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Broman KW, Wu H, Sen S, Churchill GA. R/qtl: QTL mapping in experimental crosses. Bioinformatics. 2003;19:889–890. doi: 10.1093/bioinformatics/btg112. [DOI] [PubMed] [Google Scholar]
  • 20.Lehmann E, D'Abrera H. Nonparametrics: Statistical Methods Based on Ranks. McGraw-Hill; 1988. [Google Scholar]
  • 21.Sen S, Churchill GA. A Statistical Framework for Quantitative Trait Mapping. Genetics. 2001;159:371–387. doi: 10.1093/genetics/159.1.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Churchill GA, Doerge RW. Empirical Threshold Values for Quantitative Triat Mapping. Genetics. 1994;138:963–971. doi: 10.1093/genetics/138.3.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Lander ES, Kruglyak L. Genetic Dissection of complex traits: guidelines for interpreting and reporting linkage results. Nat Genet. 1995;11:241–247. doi: 10.1038/ng1195-241. [DOI] [PubMed] [Google Scholar]
  • 24.Solberg LC, Baum AE, Ahmadiyeh N, et al. Sex- and lineage-specific inheritance of depression-like behavior in the rat. Mamm Genome. 2004;15:648–662. doi: 10.1007/s00335-004-2326-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Cho YS, Go MJ, Kim YJ, et al. A large-scale genome-wide association study of Asian populations uncovers genetic factors influencing eight quantitative traits. Nat Genet. 2009;41:527–534. doi: 10.1038/ng.357. [DOI] [PubMed] [Google Scholar]
  • 26.Xiong D-H, Liu X-G, Guo Y-F, et al. Genome-wide Association and Follow-Up Replication Studies Identified ADAMTS18 and TGFBR3 as Bone Mass Candidate Genes in Different Ethnic Groups. The American Journal of Human Genetics. 2009;84:388–398. doi: 10.1016/j.ajhg.2009.01.025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Zhang YP, Deng FY, Chen Y, et al. Replication study of candidate genes/loci associated with osteoporosis based on genome-wide screening. Osteoporosis International Epub. 2009 doi: 10.1007/s00198-009-1014-y. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Benes H, Weinstein RS, Zheng W, et al. Chromosomal Mapping of Osteopenia-Associated Quantitative Trait Loci Using Closely Related Mouse Strains. Journal of Bone and Mineral Research. 2000;15:626–633. doi: 10.1359/jbmr.2000.15.4.626. [DOI] [PubMed] [Google Scholar]
  • 29.Farber C, van Nas A, Ghazalpour A, et al. An integrative genetics approach to identify candiadte genes regulating BMD: Combining linkage, gene expression, and association. J Bone Miner Res. 2009;24:105–116. doi: 10.1359/JBMR.080908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Drake TA, Schadt E, Hannani K, et al. Genetic loci determining bone density in mice with diet-induced atherosclerosis. Physiol Genomics. 2001;5:205–215. doi: 10.1152/physiolgenomics.2001.5.4.205. [DOI] [PubMed] [Google Scholar]
  • 31.Wergedal J, Ackert-Bicknell C, Tsaih S, et al. Femur mechanical properties in the F2 progeny of an NZB/B1NJ x RF/J cross are regulated predominantly by genetic loci that regulate bone geometry. J Bone Miner Res. 2006;21:1256–1266. doi: 10.1359/jbmr.060510. [DOI] [PubMed] [Google Scholar]
  • 32.Li X, Masinde G, Gu W, Wergedal JR, Mohan S, Baylink DJ. Genetic dissection of femur breaking strength in a large population (MRL/MpJ X SJL/J) of F2 mice: single QTL effects, epistasis and pleiotrophy. Genomics. 2002;79:734–740. doi: 10.1006/geno.2002.6760. [DOI] [PubMed] [Google Scholar]
  • 33.Masinde G, Li X, Gu W, Wergedal J, Mohan S, Baylink DJ. Quantitative trait loci for bone density in mice: the genes determining total skeletal density and femur density show little overlap in F2 mice. Calcif Tissue Int. 2002;20:209–215. doi: 10.1007/s00223-001-1113-z. [DOI] [PubMed] [Google Scholar]
  • 34.Beamer WG, Shultz KL, Donahue LR, et al. Quantitative trait loci for femoral and lumbar vertebral bone mineral density in C57BL/6J and C3H/HeJ inbred strains of mice. J Bone Miner Res. 2001;16:1195–1206. doi: 10.1359/jbmr.2001.16.7.1195. [DOI] [PubMed] [Google Scholar]
  • 35.Ishimori N, Li R, Walsh K, et al. Quantitative trait loci that determine BMD in C57BL/6J and 129S1/SvImJ inbred mice. J Bone Miner Res. 2006;21:105–113. doi: 10.1359/JBMR.050902. [DOI] [PubMed] [Google Scholar]
  • 36.Beamer W, Shultz KL, Churchill GA, et al. Quantitative trait loci for bone density in C57BL/6J and CAST/EiJ. Mammalian Genome. 1999;10:1043–1049. doi: 10.1007/s003359901159. [DOI] [PubMed] [Google Scholar]
  • 37.Ackert-Bicknell C, Demissie S, Evsikova C, et al. A PPARG by dietary fat interactions influences bone mass in mice and in humans. J Bone Miner Res. 2008;23:1398–1408. doi: 10.1359/JBMR.080419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Beamer WG, Donahue LR, Rosen CJ, Baylink DJ. Genetic variability in adult bone density amoung inbred strains of mice. Bone. 1996;18:397–403. doi: 10.1016/8756-3282(96)00047-6. [DOI] [PubMed] [Google Scholar]
  • 39.Yu H, Mohan S, Edderkaoui B, et al. Detecting novel bone density and bone size quantitative trait loci using a cross of MRL/MpJ and CAST/EiJ inbred mice. Calcif Tissue Int. 2007;80:103–110. doi: 10.1007/s00223-006-0187-z. [DOI] [PubMed] [Google Scholar]
  • 40.Mohan S, Masinde G, Li X, Baylink DJ. Mapping Quantitative Trait Loci That Influence Serum Insulin-Like Growth Factor Binding Protein-5 Levels in F2 Mice (MRL/MpJ X SJL/J) Endocrinology. 2003;144:3491–3496. doi: 10.1210/en.2003-0042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Ackert-Bicknell CL, Shockley KR, Horton LG, Lecka-Czernik B, Churchill GA, Rosen CJ. Strain-Specific Effects of Rosiglitazone on Bone Mass, Body Composition, and Serum Insulin-Like Growth Factor-I. Endocrinology. 2009;150:1330–1340. doi: 10.1210/en.2008-0936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 42.Liu YJ, Shen H, Xiao P, et al. Molecular genetic studies of gene identification for osteoporosis: a 2004 update. J Bone Miner Res. 2006;21:1511–1535. doi: 10.1359/JBMR.051002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Ioannidis JP, Ng MY, Sham PC, et al. Meta-Analysis of Genome-Wide Scans Provides Evidence for Sex- and Site-Specific Regulation of Bone Mass. Journal of Bone and Mineral Research. 2007;22:173–183. doi: 10.1359/jbmr.060806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Lee YH, Rho YH, Choi SJ, Ji JD, Song GG. Meta-analysis of genome-wide linkage studies for bone mineral density. J Hum Genet. 2006;51:480–486. doi: 10.1007/s10038-006-0390-9. [DOI] [PubMed] [Google Scholar]
  • 45.Beamer WG, Shultz KL, Ackert-Bicknell CL, et al. Genetic Dissection of Mouse Distal Chromosome 1 Reveals Three Linked BMD QTLs With Sex-Dependent Regulation of Bone Phenotypes. Journal of Bone and Mineral Research. 2007;22:1187–1196. doi: 10.1359/jbmr.070419. [DOI] [PubMed] [Google Scholar]
  • 46.Edderkaoui B, Baylink DJ, Beamer WG, Shultz KL, Wergedal JR, Mohan S. Genetic regulation of femoral bone mineral density: Complexity of sex effect and in chromosome 1 revealed by congenic sublines of mice. Bone. 2007;41:340–345. doi: 10.1016/j.bone.2007.05.013. [DOI] [PubMed] [Google Scholar]
  • 47.Edderkaoui B, Baylink DJ, Beamer WG, et al. Multiple genetic loci from CAST/EiJ chromosome 1 affect vBMD either positively or negatively in a C57BL/6J background. J Bone Miner Res. 2006;21:97–104. doi: 10.1359/JBMR.051008. [DOI] [PubMed] [Google Scholar]
  • 48.Shultz KL, Donahue LR, Bouxsein ML, Baylink DJ, Rosen CJ, Beamer WG. Congenic strains of mice for varification and genetic decomposition of quantitative trait loci for femoral bone mineral density. J Bone Miner Res. 2003;18:175–185. doi: 10.1359/jbmr.2003.18.2.175. [DOI] [PubMed] [Google Scholar]
  • 49.Edderkaoui B, Baylink DJ, Beamer WG, et al. Identification of mouse Duffy Antigen Receptor for Chemokines (Darc) as a BMD QTL gene. Genome Res. 2007;17:577–585. doi: 10.1101/gr.6009507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 50.Cheung CL, Chan V, Kung AW. A differential association of ALOX15 polymorphisms with bone mineral density in pre- and post-menopausal women. Hum Hered. 2008;65:1–8. doi: 10.1159/000106057. [DOI] [PubMed] [Google Scholar]
  • 51.Leupin O, Kramer I, Collette NM, et al. Control of the SOST Bone Enhancer by PTH Using MEF2 Transcription Factors. Journal of Bone and Mineral Research. 2007;22:1957–1967. doi: 10.1359/jbmr.070804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 52.Vitarius JA, Sehayek E, Breslow JL. Identification of quantitative trait loci affecting body composition in a mouse intercross. Proceedings of the National Academy of Sciences. 2006;103:19860–19865. doi: 10.1073/pnas.0609232103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 53.Masinde GL, Li X, Gu W, Hamilton-Ulland M, Mohan S, Baylink DJ. Quantitative trait loci that harbor genes regulating muscle size in (MRL/MPJ x SJL/J) F(2) mice. Funct Integr Genomics. 2002;2:120–125. doi: 10.1007/s10142-002-0067-1. [DOI] [PubMed] [Google Scholar]
  • 54.Koller D, Schriefer J, Sun Q, et al. Genetic effects for femoral biomechanics, structure, and density in C57BL/6J and C3H/HeJ inbred mouse strains. J Bone Miner Res. 2003;18:1758–1765. doi: 10.1359/jbmr.2003.18.10.1758. [DOI] [PubMed] [Google Scholar]
  • 55.Klein RF. Genetic regulation of bone mineral density in mice. J Musculoskelet Neuronal Interact. 2002;2:232–236. [PubMed] [Google Scholar]
  • 56.Klein RF, Carlos AS, Vartanian KA, et al. Confirmation and fine mapping of chromosomal regions influencing peak bone mass in mice. J Bone Miner Res. 2001;16:1953–1961. doi: 10.1359/jbmr.2001.16.11.1953. [DOI] [PubMed] [Google Scholar]
  • 57.Orwoll ES, Belknap JK, Klein RF. Gender Specificity in the genetic determinants of peak bone mass. J Bone Miner Res. 2001;16:1962–1971. doi: 10.1359/jbmr.2001.16.11.1962. [DOI] [PubMed] [Google Scholar]
  • 58.Ishimori N, Stylianou IM, Korstanje R, et al. Quantitative Trait Loci for Bone Mineral Density in an SM/J by NZB/BlNJ Intercross Population and Identification of Trps1 as a Probable Candidate Gene. Journal of Bone and Mineral Research. 2008;0:1–41. doi: 10.1359/JBMR.080414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Ishimori N, Li R, et al. Quantitative trait loci that determine plasma lipids and obesity in C57BL/6J and 129S1/SvImJ inbred mice. J Lipid Res. 2004;45:1624–1632. doi: 10.1194/jlr.M400098-JLR200. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bone and Mineral Research are provided here courtesy of Oxford University Press

RESOURCES