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. 1969 Oct;100(1):1–4. doi: 10.1128/jb.100.1.1-4.1969

Specificity and Evolutionary Divergence of the Antigenic Structure of the Polypeptide Chain Elongation Factors

Julian Gordon 1, Manfred Schweiger 1, Istvan Krisko 1, Curtis A Williams 1
PMCID: PMC315348  PMID: 4310079

Abstract

Antisera have been prepared against two electrophoretically homogeneous “polypeptide chain elongation factors,” T and G, from Escherichia coli. Inactivation and precipitation tests showed that these two fractions were antigenically distinct with no cross-reaction. The immune inactivation curve of G factor from E. coli was distinguishable from that of G factor from Pseudomonas fluorescens. Mammalian factors were not inhibited by antibody directed against the E. coli T and G factors. By Ouchterlony diffusion tests, the antisera also detected significant antigenic variability among different bacterial species. It is concluded that the factors have undergone considerable evolutionary divergence in their antigenic structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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