Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Oct;100(1):27–34. doi: 10.1128/jb.100.1.27-34.1969

Factors Influencing In Vitro Skin Permeability Factor Production by Vibrio cholerae

Stephen H Richardson 1
PMCID: PMC315353  PMID: 5344097

Abstract

The development of a new semisynthetic medium which stimulates in vitro production of the skin permeability factor (PF) by Vibrio cholerae is described. The effects of pH, aeration, temperature, and length of incubation on PF formation or release in strain 569B and several other strains, or both, are compared. Data are presented which show that maximal PF accumulation occurs during a transitional period of growth joining the exponential and stationary phases of the growth cycle. PF elaboration is completed well ahead of any visible signs of lysis in the culture and the PF activity appears to be proportional to the length of the linear growth phase. Possible mechanisms of toxigenicity and the nature of PF are discussed.

Full text

PDF
27

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benyajati C. Experimental cholera in humans. Br Med J. 1966 Jan 15;1(5480):140–142. doi: 10.1136/bmj.1.5480.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burrows W. Cholera toxins. Annu Rev Microbiol. 1968;22:245–268. doi: 10.1146/annurev.mi.22.100168.001333. [DOI] [PubMed] [Google Scholar]
  3. Coleman W. H., Kaur J., Iwert M. E., Kasai G. J., Burrows W. Cholera toxins: purification and preliminary characterization of ileal loop reactive type 2 toxin. J Bacteriol. 1968 Oct;96(4):1137–1143. doi: 10.1128/jb.96.4.1137-1143.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Craig J. P. A permeability factor (toxin) found in cholera stools and culture filtrates and its neutralization by convalescent cholera sera. Nature. 1965 Aug 7;207(997):614–616. doi: 10.1038/207614a0. [DOI] [PubMed] [Google Scholar]
  5. Craig J. P. Preparation of the vascular permeability factor of Vibrio cholerae. J Bacteriol. 1966 Sep;92(3):793–795. doi: 10.1128/jb.92.3.793-795.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DE S. N., GHOSE M. L., CHANDRA J. Further observations on cholera enterotoxin. Trans R Soc Trop Med Hyg. 1962 May;56:241–245. doi: 10.1016/0035-9203(62)90161-x. [DOI] [PubMed] [Google Scholar]
  7. Evans D. J., Jr, Richardson S. H. In vitro production of choleragen and vascular permeability factor by Vibrio cholerae. J Bacteriol. 1968 Jul;96(1):126–130. doi: 10.1128/jb.96.1.126-130.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FINKELSTEIN R. A., NORRIS H. T., DUTTA N. K. PATHOGENESIS EXPERIMENTAL CHOLERA IN INFANT RABBITS. I. OBSERVATIONS ON THE INTRAINTESTINAL INFECTION AND EXPERIMENTAL CHOLERA PRODUCED WITH CELL-FREE PRODUCTS. J Infect Dis. 1964 Jun;114:203–216. doi: 10.1093/infdis/114.3.203. [DOI] [PubMed] [Google Scholar]
  9. Finkelstein R. A., Atthasampunna P., Chulasamaya M., Charunmethee P. Pathogenesis of experimental cholera: biologic ativities of purified procholeragen A. J Immunol. 1966 Mar;96(3):440–449. [PubMed] [Google Scholar]
  10. Finkelstein R. A., Nye S. W., Atthasampunna P., Charunmethee P. Pathogenesis of experimental cholera. Effect of choleragen on vascular permeability. Lab Invest. 1966 Oct;15(10):1601–1609. [PubMed] [Google Scholar]
  11. GALLUT J., GIUNTINI J. ETUDE DE VIBRIO CHOLERAE AU MICROSCOPE 'ELECTRONIQUE ET RELATION ENTRE L'ASPECT MORPHOLOGIQUE ET L'AGGLUTINATION O. Bull World Health Organ. 1963;29:767–771. [PMC free article] [PubMed] [Google Scholar]
  12. Kasai G. J., Burrows W. The titration of cholera toxin and antitoxin in the rabbit ileal loop. J Infect Dis. 1966 Dec;116(5):606–614. doi: 10.1093/infdis/116.5.606. [DOI] [PubMed] [Google Scholar]
  13. Leitch G. J., Burrows W. Experimental cholera in the rabbit ligated intestine: ion and water accumulation in the duodenum, ileum and colon. J Infect Dis. 1968 Oct;118(4):349–359. doi: 10.1093/infdis/118.4.349. [DOI] [PubMed] [Google Scholar]
  14. Richardson S. H., Evans D. J., Jr Isolation of cholera toxins by dextran sulfate precipitation. J Bacteriol. 1968 Oct;96(4):1443–1445. doi: 10.1128/jb.96.4.1443-1445.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Richardson S. H. Inhibition of intestinal ion translocase enzymes by culture filtrates of Vibrio cholerae. J Bacteriol. 1966 Mar;91(3):1384–1386. doi: 10.1128/jb.91.3.1384-1386.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sack R. B., Carpenter C. C., Steenburg R. W., Pierce N. F. Experimental cholera. A canine model. Lancet. 1966 Jul 23;2(7456):206–207. doi: 10.1016/s0140-6736(66)92484-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES