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. Author manuscript; available in PMC: 2012 Aug 9.
Published in final edited form as: Circulation. 2011 Jul 18;124(6):682–694. doi: 10.1161/CIRCULATIONAHA.111.023259

Figure 7.

Figure 7

Determination of the mode of inter-domain interactions in the domain switch (interacting pair of the N-terminal and central domains) and in the 2246 domain/K201 binding domain pair of WT and KI RyR2-s. The slope of the Stern-Volmer plots of the fluorescence quenching data with QSY-BSA(KQ) corresponds with the accessibility of the quencher and serves as a measure of the magnitude of domain unzipping. Effect of DPc10 or DP2246 on the KQ in WT (A) or KI (B) SR; MCA labeling was done in a site-directed manner using DPc10 or DP2246 as a carrier. The data are shown as means±SE of 5 to 6 experiments using 3 to 4 SR preparations from 30 to 40 mouse hearts.