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SDS-PAGE analysis (Coomassie staining) of 2 μg HisU directly applied (lane 2) or enriched from 400 μL of a 5 μg/mL protein solution using 2 mg of Ni2+-NTA-SA-PHEMA-SiO2-Fe3O4 beads (lane 3) or 2 mg of Dynabeads® (lane 4). Lane 1 shows a standard protein ladder.
