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. 1969 Oct;100(1):180–186. doi: 10.1128/jb.100.1.180-186.1969

Acetate Utilization and Macromolecular Synthesis During Sporulation of Yeast

Michael S Esposito a,1, Rochelle E Esposito a,2, Maryvonne Arnaud a, H O Halvorson a
PMCID: PMC315375  PMID: 5344095

Abstract

Acetate utilization and macromolecule synthesis during sporulation (meiosis) of Saccharomyces cerevisiae were studied. When diploid cells are transferred from glucose nutrient medium to acetate sporulation medium at early stationary phase, respiration of the exogenously supplied acetate proceeds without any apparent lag. At the completion of ascospore development, 62% of the acetate carbon consumed has been respired, 22% remains in the soluble pool, and 16% is incorporated into lipids, protein, nucleic acids, and other cell components. Measurements of the rate of protein synthesis during sporulation reveal two periods of maximal synthetic activity: an early phase coincidental with increases in deoxyribonucleic acid, ribonucleic acid, and protein cellular content and a later phase during ascospore formation. Experiments in which protein synthesis was inhibited at intervals during sporulation indicate that protein synthesis is required both for the initiation and completion of ascus development.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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