Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Oct;100(1):276–282. doi: 10.1128/jb.100.1.276-282.1969

Influence of Carbon or Nitrogen Starvation on Amino Acid Transport in Pseudomonas aeruginosa

W W Kay a,1, Audrey F Gronlund a
PMCID: PMC315389  PMID: 4981058

Abstract

Pseudomonas aeruginosa was shown to utilize the majority of commonly occurring amino acids for growth as either the sole carbon or the sole nitrogen source. During carbon or nitrogen deprivation, the rates of transport of most of the amino acids remained unchanged; however, the transport rates for glutamate, alanine, and glycine increased under these conditions and the transport rates for leucine and valine decreased. Normal transport rates for these amino acids were resumed immediately upon the addition of the required nutrient. In the absence of an external source of carbon or of nitrogen, pool amino acids underwent rapid degradation. 14C-Amino acid pulse experiments indicated that the constitutive amino acid catabolic enzymes, normally present in the organism during growth with glucose as the carbon source, were responsible for rapid pool losses. Nutrient starvation in the presence of chloramphenicol did not prevent amino acid catabolism. This enzymic activity is interpreted as providing P. aeruginosa with a selective advantage for survival during conditions of carbon or nitrogen starvation.

Full text

PDF
276

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRITTEN R. J., McCLURE F. T. The amino acid pool in Escherichia coli. Bacteriol Rev. 1962 Sep;26:292–335. doi: 10.1128/br.26.3.292-335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHEN G. N., MONOD J. Bacterial permeases. Bacteriol Rev. 1957 Sep;21(3):169–194. doi: 10.1128/br.21.3.169-194.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gronlund A. F., Campbell J. J. Enzymatic Degradation of Ribosomes During Endogenous Respiration of Pseudomonas aeruginosa. J Bacteriol. 1965 Jul;90(1):1–7. doi: 10.1128/jb.90.1.1-7.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Halpern Y. S., Even-Shoshan A. Properties of the glutamate transport system in Escherichia coli. J Bacteriol. 1967 Mar;93(3):1009–1016. doi: 10.1128/jb.93.3.1009-1016.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Halpern Y. S., Lupo M. Glutamate transport in wild-type and mutant strains of Escherichia coli. J Bacteriol. 1965 Nov;90(5):1288–1295. doi: 10.1128/jb.90.5.1288-1295.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holden J. T. Restoration of normal glutamic acid transport in vitamin B6-deficient Lactobacillus plantarum by acetate, ammonium, and vitamin B6. Biochim Biophys Acta. 1965 Jun 15;104(1):121–138. doi: 10.1016/0304-4165(65)90228-x. [DOI] [PubMed] [Google Scholar]
  7. Jacoby G. A. The induction and repression of amino acid oxidation in Pseudomonas fluorescens. Biochem J. 1964 Jul;92(1):1–8. doi: 10.1042/bj0920001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kay W. W., Gronlund A. F. Amino acid pool formation in Pseudomonas aeruginosa. J Bacteriol. 1969 Jan;97(1):282–291. doi: 10.1128/jb.97.1.282-291.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kay W. W., Gronlund A. F. Amino acid transport in Pseudomonas aeruginosa. J Bacteriol. 1969 Jan;97(1):273–281. doi: 10.1128/jb.97.1.273-281.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MANDELSTAM J. The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation. Bacteriol Rev. 1960 Sep;24(3):289–308. doi: 10.1128/br.24.3.289-308.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MacKelvie R. M., Campbell J. J., Gronlund A. F. Absence of storage products in cultures of Pseudomonas aeruginosa grown with excess carbon or nitrogen. Can J Microbiol. 1968 Jun;14(6):627–631. doi: 10.1139/m68-105. [DOI] [PubMed] [Google Scholar]
  12. MacKelvie R. M., Campbell J. J., Gronlund A. F. Survival and intracellular changes of Pseudomonas aeruginosa during prolonged starvation. Can J Microbiol. 1968 Jun;14(6):639–645. doi: 10.1139/m68-107. [DOI] [PubMed] [Google Scholar]
  13. McGrew S. B., Mallette M. F. ENERGY OF MAINTENANCE IN ESCHERICHIA COLI. J Bacteriol. 1962 Apr;83(4):844–850. doi: 10.1128/jb.83.4.844-850.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schlessinger D., Ben-Hamida F. Turnover of protein in Escherichia coli starving for nitrogen. Biochim Biophys Acta. 1966 Apr 18;119(1):171–182. doi: 10.1016/0005-2787(66)90048-7. [DOI] [PubMed] [Google Scholar]
  15. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES