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. 1969 Oct;100(1):475–479. doi: 10.1128/jb.100.1.475-479.1969

Endogenous Encystment of Azotobacter vinelandii

Orville Wyss 1, D D Smith 1, Leodocia M Pope 1, K E Olson 1
PMCID: PMC315416  PMID: 5344107

Abstract

When young cells of Azotobacter vinelandii are impinged on membrane filters, washed free of carbon substrate, and placed on a mineral salts basal medium, the culture will proceed to encyst although at a slower rate than if n-butanol were supplied as a substrate. The endogenous cysts are depleted in polyβ-hydroxybutyrate and have a narrower intine but show an increased resistance to desiccation and are susceptible to lysis by chelating agents. Membrane-supported cells reveal details of the encystment process such as the formation of a zone within the capsule prior to exine formation and the early deposition of exine structures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LAYNE J. S., JOHNSON E. J. NATURAL FACTORS INVOLVED IN THE INDUCTION OF CYST FORMATION IN AZOTOBACTER. J Bacteriol. 1964 Mar;87:684–689. doi: 10.1128/jb.87.3.684-689.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lin L. P., Sadoff H. L. Encystment and polymer production by Azotobacter vinelandii in the presence of beta-hydroxybutyrate. J Bacteriol. 1968 Jun;95(6):2336–2343. doi: 10.1128/jb.95.6.2336-2343.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. MOLLENHAUER H. H. Permanganate fixation of plant cells. J Biophys Biochem Cytol. 1959 Dec;6:431–436. doi: 10.1083/jcb.6.3.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Olson K. E., Wyss O. Fractionation of nucleic acids from dormant and germinated Azotobacter cysts. Biochem Biophys Res Commun. 1969 Jun 6;35(5):713–720. doi: 10.1016/0006-291x(69)90464-1. [DOI] [PubMed] [Google Scholar]
  5. Parker L. T., Socolofsky M. D. Central body of the Azotobacter cyst. J Bacteriol. 1966 Jan;91(1):297–303. doi: 10.1128/jb.91.1.297-303.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SOCOLOFSKY M. D., WYSS O. Resistance of the Azotobacter cyst. J Bacteriol. 1962 Jul;84:119–124. doi: 10.1128/jb.84.1.119-124.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sobek J. M., Charba J. F., Foust W. N. Endogenous metabolism of Azotobacter agilis. J Bacteriol. 1966 Sep;92(3):687–695. doi: 10.1128/jb.92.3.687-695.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Socolofsky M. D., Wyss O. CYSTS OF AZOTOBACTER. J Bacteriol. 1961 Jun;81(6):946–954. doi: 10.1128/jb.81.6.946-954.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stevenson L. H., Socolofsky M. D. Cyst formation and poly-beta-hydroxybutyric acid accumulation in Azotobacter. J Bacteriol. 1966 Jan;91(1):304–310. doi: 10.1128/jb.91.1.304-310.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. VELA G. R., WYSS O. IMPROVED STAIN FOR VISUALIZATION OF AZOTOBACTER ENCYSTMENT. J Bacteriol. 1964 Feb;87:476–477. doi: 10.1128/jb.87.2.476-477.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WYSS O., NEUMNN M. G., SOCOLOFSKY M. D. Development and germination of the Azotobacter cyst. J Biophys Biochem Cytol. 1961 Aug;10:555–565. doi: 10.1083/jcb.10.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]

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