Abstract
A new facultatively autotrophic Thiobacillus has been isolated in pure culture. The general physiological characteristics of the organism are described together with a redescription of Thiobacillus novellus. The new isolate differs from T. novellus in its ability to grow heterotrophically at faster rates and on a greater range of organic compounds. It can be transferred readily between autotrophic and heterotrophic conditions. It can grow anaerobically by nitrate respiration on a number of organic compounds, but not on thiosulfate. Some problems in the nomenclature and taxonomy of the thiobacilli are discussed with reference to the new isolate.
Full text
PDF










Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aleem M. I. Generation of reducing power in chemosynthesis. IV. Energy-linked reduction of pyridine nucleotides by succinate in Thiobacillus novellus. Biochim Biophys Acta. 1966 Oct 17;128(1):1–12. doi: 10.1016/0926-6593(66)90136-6. [DOI] [PubMed] [Google Scholar]
- Aleem M. I., Huang E. Carbon dioxide fixation and carboxydismutase in Thiobacillus novellus. Biochem Biophys Res Commun. 1965 Aug 16;20(4):515–520. doi: 10.1016/0006-291x(65)90610-8. [DOI] [PubMed] [Google Scholar]
- Aleem M. I. Thiosulfate Oxidation and Electron Transport in Thiobacillus novellus. J Bacteriol. 1965 Jul;90(1):95–101. doi: 10.1128/jb.90.1.95-101.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BAALSRUD K., BAALSRUD K. S. Studies on Thiobacillus denitrificans. Arch Mikrobiol. 1954;20(1):34–62. doi: 10.1007/BF00412265. [DOI] [PubMed] [Google Scholar]
- Barridge J. K., Shively J. M. Phospholipids of the thiobacilli. J Bacteriol. 1968 Jun;95(6):2182–2185. doi: 10.1128/jb.95.6.2182-2185.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brierley J. A., Brierley C. L. Urea as a nitrogen source for thiobacilli. J Bacteriol. 1968 Aug;96(2):573–573. doi: 10.1128/jb.96.2.573-.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Charles A. M., Suzuki I. Sulfite oxidase of a facultative autotroph, Thiobacillus novellus. Biochem Biophys Res Commun. 1965 Jun 9;19(6):686–690. doi: 10.1016/0006-291x(65)90311-6. [DOI] [PubMed] [Google Scholar]
- Hutchinson M., Johnstone K. I., White D. Taxonomy of anaerobic thiobacilli. J Gen Microbiol. 1967 Apr;47(1):17–23. doi: 10.1099/00221287-47-1-17. [DOI] [PubMed] [Google Scholar]
- Hutchinson M., Johnstone K. I., White D. Taxonomy of the acidophilic thiobacilli. J Gen Microbiol. 1966 Sep;44(3):373–381. doi: 10.1099/00221287-44-3-373. [DOI] [PubMed] [Google Scholar]
- Hutchinson M., Johnstone K. I., White D. The taxonomy of certain thiobacilli. J Gen Microbiol. 1965 Dec;41(3):357–366. doi: 10.1099/00221287-41-3-357. [DOI] [PubMed] [Google Scholar]
- Jackson J. F., Moriarty D. J., Nicholas D. J. Deoxyribonucleic acid base composition and taxonomy of thiobacilli and some nitrifying bacteria. J Gen Microbiol. 1968 Aug;53(1):53–60. doi: 10.1099/00221287-53-1-53. [DOI] [PubMed] [Google Scholar]
- Kelly D. P. The incorporation of acetate by the chemoautotroph Thiobacillus neapolitanus strain C. Arch Mikrobiol. 1967;58(2):99–116. doi: 10.1007/BF00406671. [DOI] [PubMed] [Google Scholar]
- Kocur M., Martinec T., Mazanec K. Fine structure of Micrococcus denitrificans and M. halodenitrificans in relation to their taxonomy. Antonie Van Leeuwenhoek. 1968;34(1):19–26. doi: 10.1007/BF02046410. [DOI] [PubMed] [Google Scholar]
- LAW J. H., SLEPECKY R. A. Assay of poly-beta-hydroxybutyric acid. J Bacteriol. 1961 Jul;82:33–36. doi: 10.1128/jb.82.1.33-36.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- LUNDGREN D. G., ALPER R., SCHNAITMAN C., MARCHESSAULT R. H. CHARACTERIZATION OF POLY-BETA-HYDROXYBUTYRATE EXTRACTED FROM DIFFERENT BACTERIA. J Bacteriol. 1965 Jan;89:245–251. doi: 10.1128/jb.89.1.245-251.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- London J., Rittenberg S. C. Thiobacillus perometabolis nov. sp., a non-autotrophic thiobacillus. Arch Mikrobiol. 1967;59(1):218–225. doi: 10.1007/BF00406335. [DOI] [PubMed] [Google Scholar]
- Léjohn H. B., Van Caeseele L., Lees H. Catabolite repression in the facultative chemoautotroph Thiobacillus novellus. J Bacteriol. 1967 Nov;94(5):1484–1491. doi: 10.1128/jb.94.5.1484-1491.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PARKER C. D., PRISK J. The oxidation of inorganic compounds of sulphur by various sulphur bacteria. J Gen Microbiol. 1953 Jun;8(3):344–364. doi: 10.1099/00221287-8-3-344. [DOI] [PubMed] [Google Scholar]
- Peck H. D., Jr Energy-coupling mechanisms in chemolithotrophic bacteria. Annu Rev Microbiol. 1968;22:489–518. doi: 10.1146/annurev.mi.22.100168.002421. [DOI] [PubMed] [Google Scholar]
- Pope L., Yolton D. P., Rode L. J. Appendages of Clostridium bifermentans spores. J Bacteriol. 1967 Oct;94(4):1206–1215. doi: 10.1128/jb.94.4.1206-1215.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SANTER M., BOYER J., SANTER U. Thiobacillus novellus. I. Growth on organic and inorganic media. J Bacteriol. 1959 Aug;78:197–202. doi: 10.1128/jb.78.2.197-202.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
- Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
- Starkey R. L. Cultivation of Organisms Concerned in the Oxidation of Thiosulfate. J Bacteriol. 1934 Oct;28(4):365–386. doi: 10.1128/jb.28.4.365-386.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki I. Incorporation of atmospheric oxygen-18 into thiosulfate by the sulfur-oxidizing enzyme of Thiobacillus thiooxidans. Biochim Biophys Acta. 1965 Oct 25;110(1):97–101. doi: 10.1016/s0926-6593(65)80098-4. [DOI] [PubMed] [Google Scholar]
- Suzuki I. Oxidation of elemental sulfur by an enzyme system of Thiobacillus thiooxidans. Biochim Biophys Acta. 1965 Jul 8;104(2):359–371. doi: 10.1016/0304-4165(65)90341-7. [DOI] [PubMed] [Google Scholar]
- Suzuki I., Silver M. The initial product and properties of the sulfur-oxidizing enzyme of thiobacilli. Biochim Biophys Acta. 1966 Jul 6;122(1):22–33. doi: 10.1016/0926-6593(66)90088-9. [DOI] [PubMed] [Google Scholar]
- TOBBACK P., LAUDELOUT H. POLY-BETA-HYDROXYBUTYRIC ACID IN NITROBACTER. Biochim Biophys Acta. 1965 Mar 8;97:589–590. doi: 10.1016/0304-4165(65)90173-x. [DOI] [PubMed] [Google Scholar]
- Taylor B. F., Currie M., Hoare D. S. Aromatic ring cleavage by a Thiobacillus. J Bacteriol. 1969 Feb;97(2):959–960. doi: 10.1128/jb.97.2.959-960.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trudinger P. A. Metabolism of thiosulfate and tetrathionate by heterotrophic bacteria from soil. J Bacteriol. 1967 Feb;93(2):550–559. doi: 10.1128/jb.93.2.550-559.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VISHNIAC W., SANTER M. The thiobacilli. Bacteriol Rev. 1957 Sep;21(3):195–213. doi: 10.1128/br.21.3.195-213.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]