Abstract
The sedimentation coefficient of streptolysin O as determined by sucrose density gradient ultracentrifugation is 3.7S. An approximate molecular weight of 60,500 was calculated from the sedimentation velocity, and a similar value was obtained by Sephadex gel filtration. There was no measurable difference in the sedimentation coefficient of streptolysin O in either the active or reversibly inactive forms, indicating that there were at most only minor conformational differences between the two forms.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALOUF J. E., RAYNAUD M. Purification of streptolysin O. Nature. 1962 Oct 27;196:374–375. doi: 10.1038/196374a0. [DOI] [PubMed] [Google Scholar]
- ANDERSEN B. R., VANNIER W. E. THE SEDIMENTATION PROPERTIES OF THE SKIN-SENSITIZING ANTIBODIES OF RAGWEED-SENSITIVE PATIENTS. J Exp Med. 1964 Jul 1;120:31–43. doi: 10.1084/jem.120.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alouf J. E., Raynaud M. Action de la streptolysine O sur les membranes cellulaires. I. Fixation sur la membrane érythrocytaire. Ann Inst Pasteur (Paris) 1968 Jun;114(6):812–827. [PubMed] [Google Scholar]
- Alouf J., Raynaud M. Nouvelle méthode de purification de la streptolysine O. C R Acad Sci Hebd Seances Acad Sci D. 1967 May 22;264(21):2524–2525. [PubMed] [Google Scholar]
- Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. Properties of the separated chains of human gamma-globulin. Nature. 1963 Jan 19;197:253–255. doi: 10.1038/197253a0. [DOI] [PubMed] [Google Scholar]
- MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]