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. 1965 Jan;89(1):23–27. doi: 10.1128/jb.89.1.23-27.1965

Multiple Cellulase System from Streptomyces antibioticus1

M D Enger a,2, B P Sleeper a
PMCID: PMC315543  PMID: 14255670

Abstract

Enger, M. D. (North Dakota State University, Fargo), and B. P. Sleeper. Multiple cellulase system from Streptomyces antibioticus. J. Bacteriol. 89:23–27. 1965.—Starch-block zone electrophoresis was used to isolate five electrophoretically distinct, active cellulolytic components (I to V) from the crude extracellular cellulase system of Streptomyces antibioticus (strain C2A). Agar diffusion precipitin analyses demonstrated the immunological identity of components I, II, and III, and the nonidentity of IV and V with each other and with I to III. Kinetic studies of the purified enzymes showed a sharp decrease in the viscosity of the substrate, carboxymethylcellulose, with only a small increase in reducing sugars. These results indicated that all five enzymes are endocellulases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CROWLE A. J. Interpretation of immunodiffusion tests. Annu Rev Microbiol. 1960;14:161–176. doi: 10.1146/annurev.mi.14.100160.001113. [DOI] [PubMed] [Google Scholar]
  2. GILLIGAN W., REESE E. T. Evidence for multiple components in microbial cellulases. Can J Microbiol. 1954 Oct;1(2):90–107. doi: 10.1139/m55-013. [DOI] [PubMed] [Google Scholar]
  3. HANSTEIN E. G., WHITAKER D. R. Improved procedures for preparation and characterization of myrothecium cellulase. 4. Characterization of activity toward beta-methyl glycosides of 1--4-beta-d-oligoglucosides. Can J Biochem Physiol. 1963 Mar;41:707–718. [PubMed] [Google Scholar]
  4. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MANDELS M., MILLER G. L., SLATER R. W., Jr Separation of fungal carbohydrases by starch blockzone electrophoresis. Arch Biochem Biophys. 1961 Apr;93:115–121. doi: 10.1016/0003-9861(61)90322-8. [DOI] [PubMed] [Google Scholar]
  6. Markert C. L. Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits. Science. 1963 Jun 21;140(3573):1329–1330. doi: 10.1126/science.140.3573.1329. [DOI] [PubMed] [Google Scholar]
  7. PETTERSSON G., PORATH J. Studies on cellulolytic enzymes. II. Multiplicity of the cellulolytic enzymes of Polyporus versicolor. Biochim Biophys Acta. 1963 Jan 8;67:9–15. doi: 10.1016/0006-3002(63)91792-x. [DOI] [PubMed] [Google Scholar]
  8. STORVICK W. O., KING K. W. The complexity and mode of action of the cellulase system of Cellvibrio gilvus. J Biol Chem. 1960 Feb;235:303–307. [PubMed] [Google Scholar]
  9. WHITAKER D. R., HANSON K. R., DATTA P. K. Improved procedures for preparation and characterization of myrothecium cellulase. 2. Purification procedures. Can J Biochem Physiol. 1963 Mar;41:671–696. [PubMed] [Google Scholar]
  10. WHITAKER D. R. Hydrolysis of a series of beta-1,4'-oligoglucosides by Myrothecium verrucaria cellulase. Arch Biochem Biophys. 1954 Dec;53(2):439–449. doi: 10.1016/0003-9861(54)90425-7. [DOI] [PubMed] [Google Scholar]
  11. WHITAKER D. R., MERLER E. Cleavage of cellotriose by Myrothecium cellulase. Can J Biochem Physiol. 1956 Jan;34(1):83–89. [PubMed] [Google Scholar]
  12. YAKULIS V. J., HELLER P. Rapid slide technic for double diffusion agar precipitin test. Am J Clin Pathol. 1959 Apr;31(4):323–325. doi: 10.1093/ajcp/31.4.323. [DOI] [PubMed] [Google Scholar]

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