Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1965 Jan;89(1):58–63. doi: 10.1128/jb.89.1.58-63.1965

Biochemical and Genetic Grouping of Isoleucine-Valine Mutants of Pseudomonas aeruginosa

L E Pearce 1, J S Loutit 1
PMCID: PMC315548  PMID: 14255682

Abstract

Pearce, L. E. (University of Otago, Dunedin, New Zealand), and J. S. Loutit. Biochemical and genetic grouping of isoleucine-valine mutants of Pseudomonas aeruginosa. J. Bacteriol. 89:58–63. 1965.—Isoleucine- and valine-requiring mutants of Pseudomonas aeruginosa, induced with ultraviolet and ethyl-methane-sulfonate, were isolated. The feeding properties and growth requirements of 56 of these mutants were examined, and evidence was found for at least four phenotypic groups, A, B, C, and D, controlling the biosynthesis of isoleucine and valine. Preliminary genetic mapping by use of bacteriophage-mediated transduction showed linkage between the mutant loci within groups A, B, and C. There was also evidence of linkage between the genes controlling groups A and B and some slight evidence of linkage between the other groups. There was insufficient evidence to suggest the arrangement of the genes.

Full text

PDF
58

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., MYERS J. W. Modification of the penicillin technique for the selection of auxotrophic bacteria. J Bacteriol. 1953 Mar;65(3):348–353. doi: 10.1128/jb.65.3.348-353.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ARMSTRONG F. B., WAGNER R. P. Biosynthesis of valine and isoleucine. V. Electrophoretic studies on the reductoisomerase and reductase of Salmonella. J Biol Chem. 1961 Dec;236:3252–3256. [PubMed] [Google Scholar]
  3. Armstrong F B, Wagner R P. Mutants of Salmonella Deficient in Reductoisomerase. Genetics. 1962 Nov;47(11):1581–1593. doi: 10.1093/genetics/47.11.1581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glanville E V, Demerec M. Threonine, Isoleucine, and Isoleucine-Valine Mutants of Salmonella Typhimurium. Genetics. 1960 Oct;45(10):1359–1374. doi: 10.1093/genetics/45.10.1359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOLLOWAY B. W., HODGINS L., FARGIE B. UNLINKED LOCI AFFECTING RELATED BIOSYNTHETIC STEPS IN PSEUDOMONAS AERUGINOSA. Nature. 1963 Aug 31;199:926–927. doi: 10.1038/199926a0. [DOI] [PubMed] [Google Scholar]
  7. HOLLOWAY B. W., MONK M., HODGINS L., FARGIE B. Effects of radiation on transduction on Pseudomonas aeruginosa. Virology. 1962 Sep;18:89–94. doi: 10.1016/0042-6822(62)90180-0. [DOI] [PubMed] [Google Scholar]
  8. HOLLOWAY B. W., MONK M. Transduction in Pseudomonas aeruginosa. Nature. 1959 Oct 31;184(Suppl 18):1426–1427. doi: 10.1038/1841426b0. [DOI] [PubMed] [Google Scholar]
  9. KAKAR S. N., WAGNER R. P. GENETIC AND BIOCHEMICAL ANALYSIS OF ISOLEUCINE-VALINE MUTANTS OF YEAST. Genetics. 1964 Feb;49:213–222. doi: 10.1093/genetics/49.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LEAVITT R. I., UMBARGER H. E. Isoleucine and valine metabolism in Escherichia coli. XI. Valine inhibition of the growth of Escherichia coli strain K-12. J Bacteriol. 1962 Mar;83:624–630. doi: 10.1128/jb.83.3.624-630.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOUTIT J. S. A transduction-like process within a single strain of Pseudomonas aeruginosa. J Gen Microbiol. 1958 Apr;18(2):315–319. doi: 10.1099/00221287-18-2-315. [DOI] [PubMed] [Google Scholar]
  12. LOUTIT J. S. Effect of ultra-violet irradiation on transduction in Pseudomonas aerginosa. Nature. 1959 Dec 19;184(Suppl 25):1960–1961. doi: 10.1038/1841960a0. [DOI] [PubMed] [Google Scholar]
  13. LOVELESS A., HOWARTH S. Mutation of bacteria at high levels of survival by ethyl methane sulphonate. Nature. 1959 Dec 5;184:1780–1782. doi: 10.1038/1841780a0. [DOI] [PubMed] [Google Scholar]
  14. RADHAKRISHANAN A. N., WAGNER R. P., SNELL E. E. Biosynthesis of valine and i43soleucine, 3. alpha-Keto-beta-hydroxy acid reductase and alpha-hydroxy-beta-Keto acid reductoisomerase. J Biol Chem. 1960 Aug;235:2322–2331. [PubMed] [Google Scholar]
  15. WAGNER R. P., BERGQUIST A. Synthesis of valine and isoleucine in the presence of a particulate cell fraction of Neurospora. Proc Natl Acad Sci U S A. 1963 Jun;49:892–897. doi: 10.1073/pnas.49.6.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wagner R P, Bergquist A. Nature of the Genetic Blocks in the Isoleucine-Valine Mutants of Salmonella. Genetics. 1960 Oct;45(10):1375–1386. doi: 10.1093/genetics/45.10.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES