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. 1965 Jan;89(1):64–73. doi: 10.1128/jb.89.1.64-73.1965

Intracellular Fate of Mengo Virus Ribonucleic Acid

M Homma a,1, A F Graham a
PMCID: PMC315549  PMID: 14255683

Abstract

Homma, M. (The Wistar Institute, Philadelphia, Pa.), and A. F. Graham. Intracellular fate of Mengo virus ribonucleic acid. J. Bacteriol. 89:64–73. 1965.—P32-labeled, purified preparations of Mengo virus adsorbed rapidly and irreversibly to L cells maintained in suspension cultures. At intervals after adsorption of labeled virus, the total ribonucleic acid (RNA) of infected cells was extracted by a phenol technique. Infectivity titrations on this RNA showed that it retained its full biological activity during the early part of the eclipse period. Sucrose gradient sedimentation analyses showed also that this RNA lost none of its structural integrity throughout the eclipse period. No evidence was found for a double-stranded structure involving parental RNA. When cells infected with P32-labeled virus were broken open during the first 7 hr after infection, no more than 20% of the parental RNA could be digested with ribonuclease. Electron microscopy indicated that only one particle in five of the purified viral populations was infectious. It is suggested that only one of five adsorbed virus particles was uncoated and that its RNA remained in the cell in an undegraded form during the eclipse period. The other adsorbed particles were not uncoated and took no part in the process of infection. The maximal transfer of parental RNA to progeny virus was 4.5%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALTIMORE D., BECKER Y., DARNELL J. E. VIRUS-SPECIFIC DOUBLE-STRANDED RNA IN POLIOVIRUS-INFECTED CELLS. Science. 1964 Mar 6;143(3610):1034–1036. doi: 10.1126/science.143.3610.1034. [DOI] [PubMed] [Google Scholar]
  2. DAVIS J. E., SINSHEIMER R. L. The replication of bacteriophage MS2. 1. Transfer of parental nucleic acid to progeny phage. J Mol Biol. 1963 Mar;6:203–207. doi: 10.1016/s0022-2836(63)80069-8. [DOI] [PubMed] [Google Scholar]
  3. DOI R. H., SPIEGELMAN S. Conservation of a viral RNA genome during replication and translation. Proc Natl Acad Sci U S A. 1963 Mar 15;49:353–360. doi: 10.1073/pnas.49.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EASON R., CLINE M. J., SMELLIE R. M. RIBONUCLEIC ACIDS IN VIRUS-INFECTED AND UNINFECTED KREBS II ASCITES TUMOR CELLS. J Biol Chem. 1963 Dec;238:3978–3984. [PubMed] [Google Scholar]
  5. FAULKNER P., MARTIN E. M., SVED S., VALENTINE R. C., WORK T. S. Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 2. The isolation, crystallization and chemical characterization of mouse encephalomyocarditis virus. Biochem J. 1961 Sep;80:597–605. doi: 10.1042/bj0800597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FENWICK M. L., COOPER P. D. Early interactions between poliovirus and ERK cells: some observations on the nature and significance of the rejected particles. Virology. 1962 Oct;18:212–223. doi: 10.1016/0042-6822(62)90007-7. [DOI] [PubMed] [Google Scholar]
  7. Gomatos P. J., Tamm I. THE SECONDARY STRUCTURE OF REOVIRUS RNA. Proc Natl Acad Sci U S A. 1963 May;49(5):707–714. doi: 10.1073/pnas.49.5.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HENRY C., YOUNGNER J. S. STUDIES ON THE STRUCTURE AND REPLICATION OF THE NUCLEIC ACID OF POLIOVIRUS. Virology. 1963 Oct;21:162–173. doi: 10.1016/0042-6822(63)90253-8. [DOI] [PubMed] [Google Scholar]
  9. HOLLAND J. J., HOYER B. H. Early stages of enterovirus infection. Cold Spring Harb Symp Quant Biol. 1962;27:101–112. doi: 10.1101/sqb.1962.027.001.013. [DOI] [PubMed] [Google Scholar]
  10. HOMMA M., GRAHAM A. F. SYNTHESIS OF RNA IN L CELLS INFECTED WITH MENGO VIRUS. J Cell Physiol. 1963 Oct;62:179–192. doi: 10.1002/jcp.1030620207. [DOI] [PubMed] [Google Scholar]
  11. JOKLIK W. K., DARNELL J. E., Jr The adsorption and early fate of purified poliovirus in HeLa cells. Virology. 1961 Apr;13:439–447. doi: 10.1016/0042-6822(61)90275-6. [DOI] [PubMed] [Google Scholar]
  12. KELLY R. B., SINSHEIMER R. L. A NEW RNA COMPONENT IN MS2-INFECTED CELLS. J Mol Biol. 1964 Apr;8:602–605. doi: 10.1016/s0022-2836(64)80015-2. [DOI] [PubMed] [Google Scholar]
  13. LADURON P., COCITO C. CONSERVATION AND TRANSFER OF THE RNA OF ANIMAL VIRUSES. Biochem Biophys Res Commun. 1963 Sep 10;13:32–36. doi: 10.1016/0006-291x(63)90157-8. [DOI] [PubMed] [Google Scholar]
  14. MANDEL B. The use of sodium dodecyl sulfate in studies on the interaction of poliovirus and HeLa cells. Virology. 1962 Jun;17:288–294. doi: 10.1016/0042-6822(62)90119-8. [DOI] [PubMed] [Google Scholar]
  15. MONTAGNIER L., SANDERS F. K. REPLICATIVE FORM OF ENCEPHALOMYOCARDITIS VIRUS RIBONUCLEIC ACID. Nature. 1963 Aug 17;199:664–667. doi: 10.1038/199664a0. [DOI] [PubMed] [Google Scholar]
  16. NEWTON N., BEVIS R. E. Purification of animal viruses with Zn(OH)2. Virology. 1959 Jul;8(3):344–351. doi: 10.1016/0042-6822(59)90034-0. [DOI] [PubMed] [Google Scholar]
  17. RAKE A. V., GRAHAM A. F. KINETICS OF INCORPORATION OF URIDINE-C14 INTO L CELL RNA. Biophys J. 1964 Jul;4:267–284. doi: 10.1016/s0006-3495(64)86782-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  19. TAYLOR J., GRAHAM A. F. Analysis of a plaque assay method for purified poliovirus MEF-1. Virology. 1961 Apr;13:427–438. doi: 10.1016/0042-6822(61)90274-4. [DOI] [PubMed] [Google Scholar]
  20. TOBEY R. A. MENGOVIRUS REPLICATION. I. CONSERVATION OF VIRUS RNA. Virology. 1964 May;23:10–22. doi: 10.1016/s0042-6822(64)80003-9. [DOI] [PubMed] [Google Scholar]
  21. TOBEY R. A. MENGOVIRUS REPLICATION. II. ISOLATION OF POLYRIBOSOMES CONTAINING THE INFECTING VIRAL GENOME. Virology. 1964 May;23:23–29. doi: 10.1016/s0042-6822(64)80004-0. [DOI] [PubMed] [Google Scholar]
  22. WEISSMANN C., BORST P., BURDON R. H., BILLETER M. A., OCHOA S. REPLICATION OF VIRAL RNA, III. DOUBLE-STRANDED REPLICATIVE FORM OF MSW PHAGE RNA. Proc Natl Acad Sci U S A. 1964 Apr;51:682–690. doi: 10.1073/pnas.51.4.682. [DOI] [PMC free article] [PubMed] [Google Scholar]

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