Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1965 Jan;89(1):193–197. doi: 10.1128/jb.89.1.193-197.1965

Experimental Salmonellosis IV. Lipid Content of Toxin L Obtained from Salmonella enteritidis

Masaya Kawakami 1, Susumu Mitsuhashi 1
PMCID: PMC315568  PMID: 14255662

Abstract

Kawakami, Masaya (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Experimental salmonellosis. IV. Lipid content of toxin L obtained from Salmonella enteritidis. J. Bacteriol. 89:193–197. 1965.—An improved method is described for the chromatographic purification of toxin L from virulent Salmonella enteritidis. Toxin L is heat-labile, and its toxicity is reduced by trypsin and by phospholipase A treatment. Chemical studies of chromatographically and ultracentrifugally homogeneous toxin preparations show that toxin L is a phosphorus-containing protein. The infrared-absorption spectrum of a petroleum ether extract of toxin L hydrolysate indicates that this material possesses a lipid component.

Full text

PDF
193

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. J. The estimation of phosphorus. Biochem J. 1940 Jun;34(6):858–865. doi: 10.1042/bj0340858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLUFF L. E. Immunochemical study of a bacterial endotoxin: Shigella flexneri type Z. J Exp Med. 1954 Oct 1;100(4):391–404. doi: 10.1084/jem.100.4.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KAWAKAMI M., OSAWA N., MITSUHASHI S. EXPERIMENTAL SALMONELLOSIS. III. NEW TOXIC FRACTION (L) OBTAINED FROM SALMONELLA ENTERITIDIS AND ITS IMMUNOLOGICAL PROPERTIES. J Bacteriol. 1963 Oct;86:872–879. doi: 10.1128/jb.86.4.872-879.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LUDERITZ O., WESTPHAL O., EICHENBEGER E., NETER E. Uber die Komplexbildung von bakteriellen Lipopolysacchariden mit Proteinen und Lipoiden. Biochem Z. 1958;330(1):21–33. [PubMed] [Google Scholar]
  5. MAENO H., MITSUHASHI S., OKONOGI T., HOSHI S., HOMMA M. Studies on Habu snake venom. (V). Myolysis caused by phospholipase A in Habu snake venom. Jpn J Exp Med. 1962 Feb;32:55–64. [PubMed] [Google Scholar]
  6. MESROBEANU L., MESROBEANU I., MITRICA N., CROITORESCO I., MARX A. STADE ACTUEL DES RECHERCHES SUR LES NEUROTOXINES DES GERMES GRAM-N'EGATIFS. Arch Roum Pathol Exp Microbiol. 1963 Sep;23:775–782. [PubMed] [Google Scholar]
  7. MOORE S., STEIN W. H. Procedures for the chromatographic determination of amino acids on four per cent cross-linked sulfonated polystyrene resins. J Biol Chem. 1954 Dec;211(2):893–906. [PubMed] [Google Scholar]
  8. Morgan W. T., Partridge S. M. Studies in immunochemistry: The use of phenol and of alkali in the degradation of antigenic material isolated from Bact. dysenteriae (Shiga). Biochem J. 1941 Nov;35(10-11):1140–1163. doi: 10.1042/bj0351140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morris D. L. Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent. Science. 1948 Mar 5;107(2775):254–255. doi: 10.1126/science.107.2775.254. [DOI] [PubMed] [Google Scholar]
  10. TAL C., GOEBEL W. F. On the nature of the toxic component of the somatic antigen of Shigella paradysenteriae type Z (Flexner). J Exp Med. 1950 Jul 1;92(1):25–34. doi: 10.1084/jem.92.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES