Abstract
Hoffman, Heiner (New York University, New York, N.Y.), and Michael E. Frank. Time-lapse photomicrography of cell growth and division in Escherichia coli. J. Bacteriol. 89:212–216. 1965.—Photomicrographs at 15-sec intervals of cells growing at 37 C disclosed that in a cell with a generation time of 21.0 min the processes of furrowing, cross-wall formation, and cell separation are completed within 2.5 min after the division furrow first becomes clearly visible. Among a large number of cultivations examined, only a few cells late in one microculture at 43.5 C failed to separate once the cross wall was completed. Measurements of cell lengths during a 5-min period, extending from just before to just after division, showed that elongation of the cell is a discontinuous process, although the growth rate over the 5-min period is exponential. At the time of cell division, it appears that the synthesis of cell-wall material is diverted entirely into formation of the cross wall.
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- ADAMS J. N. NUCLEAR MORPHOGENESIS DURING THE DEVELOPMENTAL CYCLES OF SOME MEMBERS OF THE GENUS NOCARDIA. J Gen Microbiol. 1963 Dec;33:429–435. doi: 10.1099/00221287-33-3-429. [DOI] [PubMed] [Google Scholar]
- Conti S. F., Gettner M. E. ELECTRON MICROSCOPY OF CELLULAR DIVISION IN ESCHERICHIA COLI. J Bacteriol. 1962 Mar;83(3):544–550. doi: 10.1128/jb.83.3.544-550.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDWARDS M. R., STEVENS R. W. FINE STRUCTURE OF LISTERIA MONOCYTOGENES. J Bacteriol. 1963 Sep;86:414–428. doi: 10.1128/jb.86.3.414-428.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOFFMAN H., FRANK M. E. "GERMINATION TUBE" GROWTH IN ESCHERICHIA COLI MICROCULTURES. J Bacteriol. 1964 Oct;88:1151–1154. doi: 10.1128/jb.88.4.1151-1154.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOFFMAN H., FRANK M. E. Form and internal structure of cellular aggregations in early Escherichia coli microcultures. J Gen Microbiol. 1961 Jul;25:353–364. doi: 10.1099/00221287-25-3-353. [DOI] [PubMed] [Google Scholar]
- HOFFMAN H., FRANK M. E. TEMPERATURE LIMITS, GENEALOGICAL ORIGIN, DEVELOPMENTAL COURSE, AND ULTIMATE FATE OF HEAT-INDUCED FILAMENTS IN ESCHERICHIA COLI MICROCULTURES. J Bacteriol. 1963 Jun;85:1221–1234. doi: 10.1128/jb.85.6.1221-1234.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOFFMAN H., FRANK M. E. TIME-LAPSE PHOTOMICROGRAPHY OF THE FORMATION OF A FREE SPHERICAL GRANULE IN AN ESCHERICHIA COLI CELL END. J Bacteriol. 1963 Nov;86:1075–1078. doi: 10.1128/jb.86.5.1075-1078.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- IMAEDA T., OGURA M. Formation of intracytoplasmic membrane system of mycobacteria related to cell division. J Bacteriol. 1963 Jan;85:150–163. doi: 10.1128/jb.85.1.150-163.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAYE J. J., CHAPMAN G. B. CYTOLOGICAL ASPECTS OF ANTIMICROBIAL ANTIBIOSIS. III. CYTOLOGICALLY DISTINGUISHABLE STAGES IN ANTIBIOTIC ACTION OF COLISTIN SULFATE ON ESCHERICHIA COLI. J Bacteriol. 1963 Sep;86:536–543. doi: 10.1128/jb.86.3.536-543.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MASON D. J., POWELSON D. M. Nuclear division as observed in live bacteria by a new technique. J Bacteriol. 1956 Apr;71(4):474–479. doi: 10.1128/jb.71.4.474-479.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MITCHISON J. M. The growth of single cells. III. Streptococcus faecalis. Exp Cell Res. 1961 Jan;22:208–225. doi: 10.1016/0014-4827(61)90099-4. [DOI] [PubMed] [Google Scholar]
- POWELL E. O. An outline of the pattern of bacterial generation times. J Gen Microbiol. 1958 Apr;18(2):382–417. doi: 10.1099/00221287-18-2-382. [DOI] [PubMed] [Google Scholar]
- SCHAECHTER M., WILLIAMSON J. P., HOOD J. R., Jr, KOCH A. L. Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962 Nov;29:421–434. doi: 10.1099/00221287-29-3-421. [DOI] [PubMed] [Google Scholar]