Abstract
Painter, B. G. (University of Minnesota, Minneapolis), and S. G. Bradley. Electron microscopic observations on actinophages for Streptomyces venezuelae. J. Bacteriol. 89:240–244. 1965.—Actinophages MSP2 and MSP8 were treated with various chemical agents to determine the phages' substructure. Normal MSP2 and MSP8, shadowed with uranium, or negatively stained with uranyl acetate or phosphotungstic acid, were tadpole-shaped. The average overall length of MSP2 was 245 mμ, and of MSP8, 206 mμ. The head width of both phages was approximately 58 mμ; the tail of MSP2 was 161 mμ long, and of MSP8, 137 mμ. Treatment of MSP2 and MSP8 with 7.5 m ammonium hydroxide at 56 C or 8 m urea at room temperature released some of the head contents, and revealed substructures in both the heads and the tails. The tails of phages treated with 7.5 m mercaptoethanol were destroyed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BACQ C. M. MORPHOLOGY OF ACTINOPHAGE PHI-17. J Gen Microbiol. 1963 Jul;32:131–133. doi: 10.1099/00221287-32-1-131. [DOI] [PubMed] [Google Scholar]
- BRADLEY D. E. A study of the negative staining process. J Gen Microbiol. 1962 Nov;29:503–516. doi: 10.1099/00221287-29-3-503. [DOI] [PubMed] [Google Scholar]
- BRADLEY D. E. The structure of coliphages. J Gen Microbiol. 1963 Jun;31:435–445. doi: 10.1099/00221287-31-3-435. [DOI] [PubMed] [Google Scholar]
- BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
- GILMOUR C. M., NOLLER E. C., WATKINS B. Studies on Streptomyces phage. I. Growth characteristics of the Streptomyces griseus host-phage system. J Bacteriol. 1959 Aug;78:186–192. doi: 10.1128/jb.78.2.186-192.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolstad R. A., Bradley S. G. Purification of Streptomyces venezuelae phage. J Bacteriol. 1964 May;87(5):1157–1161. doi: 10.1128/jb.87.5.1157-1161.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodruff H. B., Nunheimer T. D., Lee S. B. A Bacterial Virus for Actinomyces griseus. J Bacteriol. 1947 Oct;54(4):535–541. doi: 10.1128/jb.54.4.535-541.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]