Abstract
Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Evidence for a persistent viral infection in a cell line derived from Burkitt's lymphoma. J. Bacteriol. 89:252–258. 1965.—Exposure of a cell line (EB2) derived from Burkitt's lymphoma to vesicular stomatitis (VSV) and certain other viruses revealed a marked resistance of the cultures to infection. Intact, but not disintegrated lymphoma cells induced resistance to VSV in human embryonic kidney, amnion and diploid, as well as in green monkey kidney cells employed as feeder layers. A line of rabbit kidney or Earle's strain L cells was not protected under similar conditions. Depending upon the number of EB2 cells initially transferred, resistance developed within 1 day or in more than 2 weeks. Cell-free media collected from cultures of EB2 cells in the presence or absence of feeder layers of human cells were capable of conferring rapid protection against VSV to human-cell cultures but not to L cells. The protective principle complies with present criteria for an interferon. The results are compatible with the suggestion that EB2 cultures are latently infected with an as yet unidentified virus.
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- CANTELL K., PAUCKER K. QUANTITATIVE STUDIES ON VIRAL INTERFERENCE IN SUSPENDED L CELLS. IV. PRODUCTION AND ASSAY OF INTERFERON. Virology. 1963 Sep;21:11–21. doi: 10.1016/0042-6822(63)90298-8. [DOI] [PubMed] [Google Scholar]
- CANTELL K., PAUCKER K. Studies on viral interference in two lines of HeLa cells. Virology. 1963 Jan;19:81–87. doi: 10.1016/0042-6822(63)90027-8. [DOI] [PubMed] [Google Scholar]
- EPSTEIN M. A., ACHONG B. G., BARR Y. M. VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. Lancet. 1964 Mar 28;1(7335):702–703. doi: 10.1016/s0140-6736(64)91524-7. [DOI] [PubMed] [Google Scholar]
- EPSTEIN M. A., BARR Y. M. CULTIVATION IN VITRO OF HUMAN LYMPHOBLASTS FROM BURKITT'S MALIGNANT LYMPHOMA. Lancet. 1964 Feb 1;1(7327):252–253. doi: 10.1016/s0140-6736(64)92354-2. [DOI] [PubMed] [Google Scholar]
- FAWCETT D. W. Electron microscope observations on intracellular virus-like particles associated with the cells of the Lucké renal adenocarcinoma. J Biophys Biochem Cytol. 1956 Nov 25;2(6):725–741. doi: 10.1083/jcb.2.6.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FINK M. A., MALMGREN R. A., RAUSCHER F. J., ORR H. C., KARON M. APPLICATION OF IMMUNOFLUORESCENCE TO THE STUDY OF HUMAN LEUKEMIA. J Natl Cancer Inst. 1964 Sep;33:581–588. [PubMed] [Google Scholar]
- HENLE G., DEINHARDT F., BERGS V. V., HENLE W. Studies on persistent infections of tissue cultures. I. General aspects of the system. J Exp Med. 1958 Oct 1;108(4):537–560. doi: 10.1084/jem.108.4.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENLE W., HENLE G., DEINHARDT F., BERGS V. V. Studies on persistent infections of tissue cultures. IV. Evidence for the production of an interferon in MCN cells by myxoviruses. J Exp Med. 1959 Oct 1;110:525–541. doi: 10.1084/jem.110.4.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENLE W. INTERFERENCE AND INTERFERON IN PERSISTENT VIRAL INFECTIONS OF CELL CULTURES. J Immunol. 1963 Aug;91:145–150. [PubMed] [Google Scholar]
- PULVERTAFT J. V. CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA). Lancet. 1964 Feb 1;1(7327):238–240. doi: 10.1016/s0140-6736(64)92345-1. [DOI] [PubMed] [Google Scholar]
- RODRIGUEZ J. E., HENLE W. STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES. V. THE INITIAL STAGES OF INFECTION OF L(MCN) CELLS BY NEWCASTLE DISEASE VIRUS. J Exp Med. 1964 Jan 1;119:895–921. doi: 10.1084/jem.119.6.895. [DOI] [PMC free article] [PubMed] [Google Scholar]