Abstract
Formal, Samuel B. (Walter Reed Army Institute of Research, Washington, D.C.), E. H. LaBrec, Amos Palmer, and Stanley Falkow. Protection of monkeys against experimental shigellosis with attenuated vaccines. J. Bacteriol. 90:63–68. 1965.—Two Shigella flexneri 2a strains of reduced virulence were used as oral vaccines to protect monkeys against experimental challenge. One strain, a spontaneous mutant, had lost its ability to cause disease and was unable to penetrate the intestinal epithelium and reach the lamina propria. The other strain was a hybrid obtained by mating virulent S. flexneri 2a with Escherichia coli. This hybrid strain retained the capacity to penetrate the intestinal epithelium but was not able to maintain itself in the lamina propria. Five oral doses of the nonpenetrating mutant strain were required to render monkeys resistant to experimental challenge, but a single dose of the hybrid strain sufficed to protect the animals. There was some evidence that a degree of specificity was involved in the induced resistance, although neither vaccine evoked a consistent serum antibody or a detectable coproantibody response.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
- FORMAL S. B., LABREC E. H., KENT T. H., FALKOW S. ABORTIVE INTESTINAL INFECTION WITH AN ESCHERICHIA COLI-SHIGELLA FLEXNERI HYBRID STRAIN. J Bacteriol. 1965 May;89:1374–1382. doi: 10.1128/jb.89.5.1374-1382.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRETER R., GANGAROSA E. J. ORAL IMMUNIZATION AND PRODUCTION OF COPROANTIBODY IN HUMAN VOLUNTEERS. J Immunol. 1963 Dec;91:724–729. [PubMed] [Google Scholar]
- HIGGINS A. R., FLOYD T. M., KADER M. A. Studies in shigellosis. III. A controlled evaluation of a monovalent Shigella vaccine in a highly endemic environment. Am J Trop Med Hyg. 1955 Mar;4(2):281–288. [PubMed] [Google Scholar]
- Labrec E. H., Schneider H., Magnani T. J., Formal S. B. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY. J Bacteriol. 1964 Nov;88(5):1503–1518. doi: 10.1128/jb.88.5.1503-1518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAUSS K., KETYI I. SERUM- AND COPROANTIBODIES IN MICE, IMMUNIZED PERORALLY WITH SHIGELLA FLEXNERI. Z Immunitats Allergieforsch. 1964 Jul;127:37–50. [PubMed] [Google Scholar]