Abstract
Delwiche, C. C. (University of California, Davis), and M. S. Finstein. Carbon and energy sources for the nitrifying autotroph Nitrobacter. J. Bacteriol. 90:102–107. 1965.—The effect of various organic compounds on the growth and metabolism of the obligatively autotrophic nitrifying organism Nitrobacter was studied. A slight stimulation of both nitrification and growth was obtainable with a number of organic amendments, including yeast extract, Vitamin Free Casamino Acids, and some amino acids. Depending upon culture conditions, a strong stimulation of growth was obtained with acetate as an amendment to the culture solution. Several compounds, including valine, hydroxyproline, and threonine, were inhibitory at concentrations of 10−3m. The incorporation of carbon from isotopically labeled organic compounds was demonstrated. Acetate and glycine were particularly strong contributors to cell carbons. These could not substitute for carbon dioxide as a sole carbon source for growth, however, nor could any other of the carbon compounds that were tried.
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Selected References
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- ALEEM M. I., ALEXANDER M. Cell-free nitrification by Nitrobacter. J Bacteriol. 1958 Nov;76(5):510–514. doi: 10.1128/jb.76.5.510-514.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DELWICHE C. C., BURGE W. G., MALAVOLTA E. SOME ORGANIC TRANSFORMATION EFFECTED BY CELL-FREE PREPARATIONS OF NITROSOMONAS. Arch Biochem Biophys. 1963 Dec;103:500–505. doi: 10.1016/0003-9861(63)90443-0. [DOI] [PubMed] [Google Scholar]
- FINSTEIN M. S., DELWICHE C. C. MOLYBDENUM AS A MICRONUTRIENT FOR NITROBACTER. J Bacteriol. 1965 Jan;89:123–128. doi: 10.1128/jb.89.1.123-128.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ida S., Alexander M. Permeability of Nitrobacter agilis to Organic Compounds. J Bacteriol. 1965 Jul;90(1):151–156. doi: 10.1128/jb.90.1.151-156.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEES H. The biochemistry of the nitrifying organisms. I. The ammonia oxidizing systems of Nitrosomonas. Biochem J. 1952 Sep;52(1):134–139. doi: 10.1042/bj0520134. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lees H., Quastel J. H. Biochemistry of nitrification in soil: 1. Kinetics of, and the effects of poisons on, soil nitrification, as studied by a soil perfusion technique. (with an Addendum by H. Lees). Biochem J. 1946;40(5-6):803–815. doi: 10.1042/bj0400803. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lees H., Quastel J. H. Biochemistry of nitrification in soil: 3. Nitrification of various organic nitrogen compounds. Biochem J. 1946;40(5-6):824–828. [PMC free article] [PubMed] [Google Scholar]
- MALAVOLTA E., DELWICHE C. C., BURGE W. D. Formate odixation by cell-free preparations from Nitrobacter agilis. Biochim Biophys Acta. 1962 Feb 26;57:347–351. doi: 10.1016/0006-3002(62)91129-0. [DOI] [PubMed] [Google Scholar]