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. 1965 Aug;90(2):395–402. doi: 10.1128/jb.90.2.395-402.1965

Lysis of Bacterial Cell Walls by an Enzyme Isolated from a Myxobacter

J C Ensign a,1, R S Wolfe a
PMCID: PMC315657  PMID: 14330733

Abstract

Ensign, J. C. (University of Illinois, Urbana), and R. S. Wolfe. Lysis of bacterial cell walls by an enzyme isolated from a myxobacter. J. Bacteriol. 90:395–402. 1965.—An exoenzyme which lyses intact cells, heat-killed cells, and cell walls of Arthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain AL-1). The lytic enzyme was produced during growth of the organism in a number of complex media, the maximal amount of enzyme being produced in yeast extract broth. The purified enzyme lysed at different rates a number of gram-positive bacteria. With the exception of Rhodospirillum rubrum, Spirillum itersonii, and S. serpens, the gram-negative bacteria tested were not attacked.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DWORKIN M. Nutritional requirements for vegetative growth of Myxococcus xanthus. J Bacteriol. 1962 Aug;84:250–257. doi: 10.1128/jb.84.2.250-257.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ENSIGN J. C., WOLFE R. S. NUTRITIONAL CONTROL OF MORPHOGENESIS IN ARTHROBACTER CRYSTALLOPIETES. J Bacteriol. 1964 Apr;87:924–932. doi: 10.1128/jb.87.4.924-932.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GHUYSEN J. M. Activités bactériolytiques de l'actinomycetine de Streptomyces albus G. Arch Int Physiol Biochim. 1957 Apr;65(2):173–305. [PubMed] [Google Scholar]
  4. GHUYSEN J. M., STROMINGER J. L. STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. I. PREPARATION OF FRAGMENTS BY ENZYMATIC HYDROLYSIS. Biochemistry. 1963 Sep-Oct;2:1110–1119. doi: 10.1021/bi00905a035. [DOI] [PubMed] [Google Scholar]
  5. GILLESPIE D. C., COOK F. D. EXTRACELLULAR ENZYMES FROM STRAINS OF SORANGIUM. Can J Microbiol. 1965 Feb;11:109–118. doi: 10.1139/m65-014. [DOI] [PubMed] [Google Scholar]
  6. HASH J. H. PURIFICATION AND PROPERTIES OF STAPHYLOLYTIC ENZYMES FROM CHALAROPSIS SP. Arch Biochem Biophys. 1963 Sep;102:379–388. doi: 10.1016/0003-9861(63)90245-5. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. RICHMOND M. H. Formation of a lytic enzyme by a strain of Bacillus subtilis. Biochim Biophys Acta. 1959 May;33(1):78–92. doi: 10.1016/0006-3002(59)90500-1. [DOI] [PubMed] [Google Scholar]
  9. SALTON M. R. Isolation of Streptomyces spp. capable of decomposing preparations of cell walls from various micro-organisms and a comparison of their lytic activities with those of certain actinomycetes and myxobacteria. J Gen Microbiol. 1955 Feb;12(1):25–30. doi: 10.1099/00221287-12-1-25. [DOI] [PubMed] [Google Scholar]
  10. SALTON M. R., WILLIAMS R. C. Electron microscopy of the cell walls of Bacillus megaterium and Rhodospirillum rubrum. Biochim Biophys Acta. 1954 Aug;14(4):455–458. doi: 10.1016/0006-3002(54)90224-3. [DOI] [PubMed] [Google Scholar]
  11. SCHINDLER C. A., SCHUHARDT V. T. LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUS. Proc Natl Acad Sci U S A. 1964 Mar;51:414–421. doi: 10.1073/pnas.51.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stanier R. Y. Studies on Nonfruiting Myxobacteria: I. Cytophaga johnsonae, n.sp., a Chitin-decomposing Myxobacterium. J Bacteriol. 1947 Mar;53(3):297–315. [PMC free article] [PubMed] [Google Scholar]

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