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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1965 Sep;90(3):673–680. doi: 10.1128/jb.90.3.673-680.1965

Induced Biosynthesis of Formic Hydrogenlyase in Iron-Deficient Cells of Escherichia coli

T Fukuyama a,1, E J Ordal a
PMCID: PMC315709  PMID: 16562066

Abstract

Fukuyama, T. (University of Washington, Seattle), and E. J. Ordal. Induced biosynthesis of formic hydrogenlyase in iron-deficient cells of Escherichia coli. J. Bacteriol. 90:673–680. 1965.—Escherichia coli cells were grown aerobically on a lactate-mineral salts medium from which iron had been removed by extraction with 8-hydroxyquinoline and chloroform. These cells carried out induced biosynthesis of formic hydrogenlyase in a reaction mixture containing glucose, formate, and phosphate without the addition of amino acids, providing adequate amounts of iron salts were present. In the absence of iron, glucose was fermented and acids were produced, but no formic hydrogenlyase developed. When iron-deficient E. coli cells were repeatedly washed, the property of carrying out induced biosynthesis of formic hydrogenlyase with glucose, formate, phosphate, and iron was lost, but was restored on addition of acid-hydrolyzed casein to the reaction mixture. An energy source (provided as glucose) was necessary for enzyme production. Iron-deficient cells were devoid of hydrogenase and formic hydrogenlyase but showed formic dehydrogenase activity when adequate amounts of selenium and molybdenum were present in the growth medium. Hydrogenase was consistently absent in iron-deficient cells but appeared concomitantly with formic hydrogenlyase during induced biosynthesis of the latter in iron-deficient cells of E. coli.

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Selected References

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  1. BARRETT J. T., LARSON A. D., KALLIO R. E. The nature of the adaptive lag of Pseudomonas fluorescens toward citrate. J Bacteriol. 1953 Feb;65(2):187–192. doi: 10.1128/jb.65.2.187-192.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BILLEN D., LICHSTEIN H. C. Nutritional requirement for the production of formic hydrogenylase, formic dehydrogenase, and hydrogenase, in Escherichia coli. J Bacteriol. 1951 Apr;61(4):515–522. doi: 10.1128/jb.61.4.515-522.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOVELL C. R., PACKER L., HELGERSON R. PERMEABILITY OF ESCHERICHIA COLI TO ORGANIC COMPOUNDS AND INORGANIC SALTS MEASURED BY LIGHT-SCATTERING. Biochim Biophys Acta. 1963 Sep 24;75:257–266. doi: 10.1016/0006-3002(63)90604-8. [DOI] [PubMed] [Google Scholar]
  4. BRITTEN R. J., McCLURE F. T. The amino acid pool in Escherichia coli. Bacteriol Rev. 1962 Sep;26:292–335. doi: 10.1128/br.26.3.292-335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COHEN G. N., MONOD J. Bacterial permeases. Bacteriol Rev. 1957 Sep;21(3):169–194. doi: 10.1128/br.21.3.169-194.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ENGLESBERG E., WATSON J. A., HOFFEE P. A. The glucose effect and the relationship between glucose permease, acid phosphatase, and glucose resistance. Cold Spring Harb Symp Quant Biol. 1961;26:261–276. doi: 10.1101/sqb.1961.026.01.033. [DOI] [PubMed] [Google Scholar]
  7. GEST H., GIBBS M. Preparation and properties of cell-free "formic hydrogenlyase" from escherichia coli. J Bacteriol. 1952 May;63(5):661–664. doi: 10.1128/jb.63.5.661-664.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GEST H. Oxidation and evolution of molecular hydrogen by microorganisms. Bacteriol Rev. 1954 Mar;18(1):43–73. doi: 10.1128/br.18.1.43-73.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GEST H., PECK H. D., Jr A study of the hydrogenlyase reaction with systems derived from normal and anaerogenic coli-aerogenes bacteria. J Bacteriol. 1955 Sep;70(3):326–334. doi: 10.1128/jb.70.3.326-334.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KUSHNER D. J., QUASTEL J. H. Factors underlying bacterial enzyme synthesis. Proc Soc Exp Biol Med. 1953 Mar;82(3):388–392. doi: 10.3181/00379727-82-20125. [DOI] [PubMed] [Google Scholar]
  11. MANDELSTAM J. The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation. Bacteriol Rev. 1960 Sep;24(3):289–308. doi: 10.1128/br.24.3.289-308.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PECK H. D., Jr, GEST H. Formic dehydrogenase and the hydrogenlyase enzyme complex in coli-aerogenes bacteria. J Bacteriol. 1957 Jun;73(6):706–721. doi: 10.1128/jb.73.6.706-721.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PICHINOTY F. [Inhibition by oxygen of the biosynthesis and activity of hydrogenase and hydrogenlyase in some anaerobic bacteria]. Biochim Biophys Acta. 1962 Oct 8;64:111–124. doi: 10.1016/0006-3002(62)90764-3. [DOI] [PubMed] [Google Scholar]
  14. PINSENT J. The need for selenite and molybdate in the formation of formic dehydrogenase by members of the coli-aerogenes group of bacteria. Biochem J. 1954 May;57(1):10–16. doi: 10.1042/bj0570010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PINSKY M. J., STOKES J. L. Requirements for formic hydrogenlyase adaptation in nonproliferating suspensions of escherichia coli. J Bacteriol. 1952 Aug;64(2):151–161. doi: 10.1128/jb.64.2.151-161.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stephenson M., Stickland L. H. Hydrogenlyases: Bacterial enzymes liberating molecular hydrogen. Biochem J. 1932;26(3):712–724. doi: 10.1042/bj0260712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stephenson M., Stickland L. H. Hydrogenlyases: Further experiments on the formation of formic hydrogenlyase by Bact. coli. Biochem J. 1933;27(5):1528–1532. doi: 10.1042/bj0271528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. UPADHYAY J., STOKES J. L. TEMPERATURE-SENSITIVE HYDROGENASE AND HYDROGENASE SYNTHESIS IN A PSYCHROPHILIC BACTERIUM. J Bacteriol. 1963 Nov;86:992–998. doi: 10.1128/jb.86.5.992-998.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. UPADHYAY J., STOKES R. L. Temperature-sensitive formic hydrogenlyase in a psychrophilic bacterium. J Bacteriol. 1963 Jan;85:177–185. doi: 10.1128/jb.85.1.177-185.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

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