Abstract
Morris, George K. (University of Georgia, Athens), and William L. Williams. Inhibition of growth of Lactobacillus bulgaricus by purine deoxyribonucleotides. J. Bacteriol. 90:715–719. 1965.—An inhibition of growth of Lactobacillus bulgaricus GS was observed with deoxyadenylic acid and deoxyguanylic acid. Deoxynucleotides of cytosine, thymine, and uracil, and the deoxynucleosides of adenine, guanine, cytosine, and thymine were inactive as inhibitors. The inhibition was reversed by liver extract (a crude source of two unidentified growth factors for this organism). With suboptimal concentrations of liver extract, the inhibition was reversed by nucleotides of adenine, guanine, uracil, cytosine, and thymine. When the medium contained partially purified sources of the two growth factors rather than crude liver extract, fewer compounds reversed the inhibition. Adenylic acid and guanylic acid reversed the action of either inhibitor. Inosinic acid reversed inhibition caused by deoxyguanylic acid, but not that caused by deoxyadenylic acid. Thymidylic acid reversed inhibition caused by deoxyadenylic acid better than that caused by deoxyguanylic acid. Uridylic acid and cytidylic acid were no longer effective in reversing the inhibitions. This organism preferentially responded to monophosphorylated compounds as inhibitors and as reversers of inhibitions. Studies on the acid-soluble nucleotide pool revealed an accumulation of adenosine triphosphate, guanosine triphosphate, and an unidentified compound which resembled a nucleotide in its physical properties. These data cannot be explained by known metabolic pathways of nucleic acid biosynthesis. This organism responds differently from other related organisms to nucleic acid derivatives; therefore, it may be a new useful tool for studying nucleic acid metabolism and biosynthesis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HURLBERT R. B., SCHMITZ H., BRUMM A. F., POTTER V. R. Nucleotide metabolism. II. Chromatographic separation of acid-soluble nucleotides. J Biol Chem. 1954 Jul;209(1):23–39. [PubMed] [Google Scholar]
- IVES D. H., MORSE P. A., Jr, POTTER V. R. Feedback inhibition of thymodine kinase by thymodine triphosphate. J Biol Chem. 1963 Apr;238:1467–1474. [PubMed] [Google Scholar]
- KITAY E., McNUTT W. S., SNELL E. E. Desoxyribosides and vitamin B12 as growth factors for lactic acid bacteria. J Bacteriol. 1950 Jun;59(6):727–738. doi: 10.1128/jb.59.6.727-738.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LANGER L., KLENOW H. The effect of some purine deoxyribosides on the incorporation of [14C] formate and [32P] orthophosphate into DNA of ascites tumor cells in vitro. Biochim Biophys Acta. 1960 Jan 1;37:33–37. doi: 10.1016/0006-3002(60)90074-3. [DOI] [PubMed] [Google Scholar]
- REICHARD P., CANELLAKIS Z. N., CANELLAKIS E. S. Studies on a possible regulatory mechanism for the biosynthesis of deoxyribonucleic acid. J Biol Chem. 1961 Sep;236:2514–2519. [PubMed] [Google Scholar]
- SIEDLER A. J., SCHWEIGERT B. S. Influence of ribonucleotides on the utilization of deoxyribonucleotides by Lactobacillus acidophilus. J Bacteriol. 1959 Apr;77(4):514–515. doi: 10.1128/jb.77.4.514-515.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEINMAN D. E., MORRIS G. K., WILLIAMS W. L. UNIDENTIFIED GROWTH FACTOR FOR A LACTIC ACID BACTERIUM. J Bacteriol. 1964 Feb;87:263–269. doi: 10.1128/jb.87.2.263-269.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]