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. 1965 Sep;90(3):748–754. doi: 10.1128/jb.90.3.748-754.1965

Free Amino Acids in Serine-Antagonized Cells of Tetrahymena pyriformis1

June B Wragg 1,2, Howard Reynolds 1,2, Michael J Pelczar Jr 1,2
PMCID: PMC315721  PMID: 16562077

Abstract

Wragg, June B. (Agricultural Research Service, Beltsville, Md.), Howard Reynolds, and Michael J. Pelczar, Jr. Free amino acids in serine-antagonized cells of Tetrahymena pyriformis. J. Bacteriol. 90:748–754. 1965.—Growth inhibition of Tetrahymena pyriformis by l-serine in a chemically defined medium was reversed by l-arginine in a manner which resembled competitive antagonism. Composition of the free amino acid pools from cells grown in either a balanced amino acid mixture or a mixture with serine concentrations which inhibited growth suggested an antagonism by serine with energy-yielding reactions. Growth in media with excess serine resulted in the accumulation of higher concentrations of free cellular amino acids and an apparent increase in the rate of conversion of arginine to ornithine, as compared with growth in the balanced medium. The results suggested that serine or a metabolic product of serine interferes with the formation of pyruvic acid. In the presence of high levels of serine, arginine appeared to be metabolized more rapidly and to be spared when alanine, aspartic acid, or glutamic acid was added to the unbalanced medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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