In the article entitled, “A Melting Curve Analysis–Based PCR Assay for One-Step Genotyping of β-Thalassemia Mutations: A Multicenter Validation” (Volume 13, pages 427–435 of the July 2011 issue of The Journal of Molecular Diagnostics), the authors found several small, but obvious, erroneous calculations in Table 2.. The authors have since corrected the values and the corrected Table 2 appears below.
Table 2.
Calibrated Tm of 24 Mutations Detected Using the MeltPro HBB Assay
| Tube | Color | Genotype | Tm (°C) | ΔTm (3 SD) (°C)⁎ |
|---|---|---|---|---|
| 1 | FAM | Wild-type | 65.9 | NA |
| c.316-197C>T | 59.9 | 6.0 (0.42) | ||
| HEX | Wild-type | 65.4 | NA | |
| c.-78A>G | 57.0 | 8.4 (0.39) | ||
| c.-79A>G | 58.4 | 7.0 (0.54) | ||
| c.-80T>C or c.-81A>C† | 62.1 | 3.3 (0.42) | ||
| c.-82C>A | 54.3 | 11.1 (0.39) | ||
| ROX | Wild-type | 67.7 | NA | |
| c.45-46insG | 64.6 | 3.1 (0.45) | ||
| c.48-49insG | 66.0 | 1.7 (0.35) | ||
| c.52A>T | 62.2 | 5.5 (0.54) | ||
| Cy5 | Wild-type | 67.1 | NA | |
| c.79G>A | 61.7 | 5.4 (0.31) | ||
| c.92 + 1G>T or c.92 + 5G>C† | 63.4 | 3.7 (0.33) | ||
| c.91A>G | 59.2 | 7.9 (0.51) | ||
| 2 | FAM | Wild-type | 68.4 | NA |
| c.79G>A | 61.4 | 7.0 (0.36) | ||
| c.84-85insC | 64.0 | 4.4 (0.45) | ||
| HEX | Wild-type | 71.0 | NA | |
| c.124-127delTTCT | 60.2 | 10.8 (0.54) | ||
| c.115delA | 61.3 | 9.7 (0.48) | ||
| c.130G>T or c.113G>A† | 66.3 | 4.7 (0.48) | ||
| ROX | Wild-type | 68.1 | NA | |
| c.216-217insT | 63.8 | 4.3 (0.54) | ||
| c.216-217insA | 62.5 | 5.6 (0.50) | ||
| c.315 + 2delT | 48.1 | 20.0 (0.45) | ||
| c.315 + 5G>C | 51.4 | 16.7 (0.24) | ||
| Cy5 | Wild-type | 64.6 | NA | |
| c.-123A>T | 58.1 | 6.5 (0.42) | ||
| c.-140C>T | 55.7 | 8.9 (0.33) |
NA, not applicable.
ΔTm = Tm (wild-type) − Tm (mutant).
These mutations are judged to be the same type within pairs using the MeltPro HBB assay.
The article entitled “Copy Number Gains in 11q13 and 8q34 Are Highly Linked to Prognosis in Cutaneous Malignant Melanoma” (Volume 13, pages 352-358 of the May 2011 issue), contained an error in the chromosomal band for MYC in the title and throughout the text. The correct chromosomal band for MYC is 8q24. The authors apologize for the error.