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. 1965 Oct;90(4):1059–1072. doi: 10.1128/jb.90.4.1059-1072.1965

Electron Microscopy of Chromatophores of Rhodopseudomonas spheroides

K D Gibson a,1
PMCID: PMC315776  PMID: 5847796

Abstract

Gibson, K. D. (St. Mary's Hospital Medical School, London, England). Electron microscopy of Rhodopseudomonas spheroides. J. Bacteriol. 90:1059–1072. 1965.—Fixed and stained chromatophores and whole cells of anaerobically grown Rhodopseudomonas spheroides were examined in thin sections in the electron microscope. Both purified chromatophores and intracellular membrane-bound vesicles had exactly the same appearance, namely that of spheres or ellipsoids with a thin electron-dense shell surrounding an electron-lucent interior. The distribution of diameters in the two types of structure was also found to be the same, and was compatible with a normal distribution, with a mean of 570 A and a standard deviation 40 A. Negatively stained chromatophores appeared like discs or collapsed spheres. The presence of invaginations of the cytoplasmic membrane in this species was confirmed, and a new structure resembling a twin chromatophore was observed. The bearing of these results on theories of the origin of chromatophores is discussed, and it is concluded that they offer some support for each one of the three main theories about the origin of particulate organelles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAHR G. F., ZEITLER E. Study of mitochondria in rat liver. Quantitative electron microscopy. J Cell Biol. 1962 Dec;15:489–501. doi: 10.1083/jcb.15.3.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOATMAN E. S., DOUGLAS H. C. Fine structure of the photosynthetic bacterium Rhodomicrobium vannielii. J Biophys Biochem Cytol. 1961 Nov;11:469–483. doi: 10.1083/jcb.11.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOATMAN E. S. OBSERVATIONS ON THE FINE STRUCTURE OF SPHEROPLASTS OF RHODOSPIRILLUM RUBRUM. J Cell Biol. 1964 Feb;20:297–311. doi: 10.1083/jcb.20.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHAPMAN G. B., KROLL A. J. Electron microscopy of ultrathin sections of Spirillum serpens. J Bacteriol. 1957 Jan;73(1):63–71. doi: 10.1128/jb.73.1.63-71.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COHEN-BAZIRE G., KUNISAWA R. The fine structure of Rhodospirillum rubrum. J Cell Biol. 1963 Feb;16:401–419. doi: 10.1083/jcb.16.2.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COHEN-BAZIRE G., PFENNIG N., KUNISAWA R. THE FINE STRUCTURE OF GREEN BACTERIA. J Cell Biol. 1964 Jul;22:207–225. doi: 10.1083/jcb.22.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DE PETRIS S., KARLSBAD G., KESSEL R. W. THE ULTRASTRUCTURE OF S AND R VARIANTS OF BRUCELLA ABORTUS GROWN ON A LIFELESS MEDIUM. J Gen Microbiol. 1964 Jun;35:373–382. doi: 10.1099/00221287-35-3-373. [DOI] [PubMed] [Google Scholar]
  8. DREWS G. [Studies on the substructure of "chromatophores" of Rhodospirillum rubrum and Rhodospirillum molischianum]. Arch Mikrobiol. 1960;36:99–108. [PubMed] [Google Scholar]
  9. EPHRUSSI B. The interplay of heredity and environment in the synthesis of respiratory enzymes in yeast. Harvey Lect. 1950 1951;Series 46:45–67. [PubMed] [Google Scholar]
  10. FRENKEL A. W., HICKMAN D. D. Structure and photochemical activity of chlorophyll-containing particles from Rhodospirillum rubrum. J Biophys Biochem Cytol. 1959 Oct;6:285–290. doi: 10.1083/jcb.6.2.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GORCHEIN A. ORNITHINE IN RHODOPSEUDOMONAS SPHEROIDES. Biochim Biophys Acta. 1964 Jun 15;84:356–358. doi: 10.1016/0926-6542(64)90064-2. [DOI] [PubMed] [Google Scholar]
  12. HICKMAN D. D., FRENKEL A. W. The structure of Rhodospirillum rubrum. J Biophys Biochem Cytol. 1959 Oct;6:277–284. doi: 10.1083/jcb.6.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOLT S. C., MARR A. G. ISOLATION AND PURIFICATION OF THE INTRACYTOPLASMIC MEMBRANES OF RHODOSPIRILLUM RUBRUM. J Bacteriol. 1965 May;89:1413–1420. doi: 10.1128/jb.89.5.1413-1420.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LASCELLES J. Adaptation to form bacteriochlorophyll in Rhodopseudomonas spheroides: changes in activity of enzymes concerned in pyrrole synthesis. Biochem J. 1959 Jul;72:508–518. doi: 10.1042/bj0720508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LUCK D. J. Genesis of mitochondria in neurospora crassa. Proc Natl Acad Sci U S A. 1963 Feb 15;49:233–240. doi: 10.1073/pnas.49.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LUCK D. J., REICH E. DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1964 Oct;52:931–938. doi: 10.1073/pnas.52.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LUCY J. A., GLAUERT A. M. STRUCTURE AND ASSEMBLY OF MACROMOLECULAR LIPID COMPLEXES COMPOSED OF GLOBULAR MICELLES. J Mol Biol. 1964 May;8:727–748. doi: 10.1016/s0022-2836(64)80121-2. [DOI] [PubMed] [Google Scholar]
  19. NEWTON J. W., NEWTON G. A. Composition of the photoactive subcellular particles from Chromatium. Arch Biochem Biophys. 1957 Sep;71(1):250–265. doi: 10.1016/0003-9861(57)90026-7. [DOI] [PubMed] [Google Scholar]
  20. Parsons D. F. Mitochondrial Structure: Two Types of Subunits on Negatively Stained Mitochondrial Membranes. Science. 1963 May 31;140(3570):985–987. doi: 10.1126/science.140.3570.985. [DOI] [PubMed] [Google Scholar]
  21. SCHACHMAN H. K., PARDEE A. B., STANIER R. Y. Studies on the macro-molecular organization of microbial cells. Arch Biochem Biophys. 1952 Jul;38:245–260. doi: 10.1016/0003-9861(52)90029-5. [DOI] [PubMed] [Google Scholar]
  22. VATTER A. E., WOLFE R. S. The structure of photosynthetic bacteria. J Bacteriol. 1958 Apr;75(4):480–488. doi: 10.1128/jb.75.4.480-488.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. WORDEN P. B., SISTROM W. R. THE PREPARATION AND PROPERTIES OF BACTERIAL CHROMATOPHORE FRACTIONS. J Cell Biol. 1964 Oct;23:135–150. doi: 10.1083/jcb.23.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]

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