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. 1965 Oct;90(4):1102–1108. doi: 10.1128/jb.90.4.1102-1108.1965

Inhibition of Protein Synthesis by Polypeptide Antibiotics I. Inhibition in Intact Bacteria

Herbert L Ennis 1
PMCID: PMC315780  PMID: 4954517

Abstract

Ennis, Herbert L. (St. Jude Children's Research Hospital, Memphis, Tenn.). Inhibition of protein synthesis by polypeptide antibiotics. I. Inhibition in intact bacteria. J. Bacteriol. 90:1102–1108. 1965.—The mechanism of inhibition of growth of cells by the polypeptide antibiotics of the PA 114, vernamycin, and streptogramin complexes was studied. This inhibition apparently was due to the selective inhibition of protein synthesis by these antibiotics. Ribonucleic acid synthesis was unaffected by concentrations of the antibiotics which completely inhibited protein synthesis. Deoxyribonucleic acid synthesis was slightly inhibited. These antibiotics are composed of a number of components. Mixtures of equal amounts of PA 114 A and PA 114 B or vernamycin A and Bα were more active in stopping protein synthesis in intact cells than each of the components of the antibiotic complex alone. Mutants resistant to one of the antibiotics were resistant to all of the group and, in addition, were resistant to erythromycin and oleandomycin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BODANSZKY M., ONDETTI M. A. STRUCTURES OF THE VERNAMYCIN B GROUP OF ANTIBIOTICS. Antimicrob Agents Chemother (Bethesda) 1963;161:360–365. [PubMed] [Google Scholar]
  2. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ENNIS H. L., LUBIN M. PRE-RIBOSOMAL PARTICLES FORMED IN POTASSIUM-DEPLETED CELLS. STUDIES ON DEGRADATION AND STABILIZATION. Biochim Biophys Acta. 1965 Apr 19;95:605–623. doi: 10.1016/0005-2787(65)90515-0. [DOI] [PubMed] [Google Scholar]
  4. GARROD L. P., WATERWORTH P. M. Behaviour in vitro of some new antistaphylococcal antibiotics. Br Med J. 1956 Jul 14;2(4984):61–65. doi: 10.1136/bmj.2.4984.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ROODYN D. B., MANDEL H. G. A simple membrane fractionation method for determining the distribution of radioactivity in chemical fractions of Bacillus cereus. Biochim Biophys Acta. 1960 Jun 17;41:80–88. doi: 10.1016/0006-3002(60)90371-1. [DOI] [PubMed] [Google Scholar]
  6. SELLS B. H. RNA SYNTHESIS AND RIBOSOME PRODUCTION IN PUROMYCIN-TREATED CELLS. Biochim Biophys Acta. 1964 Feb 17;80:230–241. doi: 10.1016/0926-6550(64)90095-7. [DOI] [PubMed] [Google Scholar]
  7. TANAKA N., MIYAIRI N., WATANABE K., SHINJO N., NISHIMURA T., UMEZAWA H. Biological studies on mikamycin. II. Laboratory investigations of mikamycin A and mikamycin B. J Antibiot (Tokyo) 1959 Nov;12:290–297. [PubMed] [Google Scholar]
  8. TAUBMAN S. B., SO A. G., YOUNG F. E., DAVIE E. W., CORCORAN J. W. EFFECT OF ERYTHROMYCIN ON PROTEIN BIOSYNTHESIS IN BACILLUS SUBTILIS. Antimicrob Agents Chemother (Bethesda) 1963;161:395–401. [PubMed] [Google Scholar]
  9. VAZQUEZ D. Studies on the mode of action of streptogramin. Biochim Biophys Acta. 1962 Nov 26;61:849–851. doi: 10.1016/0926-6550(62)90075-0. [DOI] [PubMed] [Google Scholar]
  10. YAMAGUCHI H. Action mechanism of mikamycins. I. Effects of mikamycins on protein and nucleic acid metabolisms. J Antibiot (Tokyo) 1961 Nov;14:313–323. [PubMed] [Google Scholar]

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