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. 1965 Nov;90(5):1174–1181. doi: 10.1128/jb.90.5.1174-1181.1965

Drug Resistance of Enteric Bacteria V. High Frequency of Transduction of R Factors with Bacteriophage Epsilon

Mitsuo Kameda 1, Kenji Harada 1, Mitsue Suzuki 1, Susumu Mitsuhashi 1
PMCID: PMC315799  PMID: 5848323

Abstract

Kameda, Mitsuo (Gunma University, Maebashi, Japan), Kenji Harada, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. V. High frequency of transduction of R factors with bacteriophage epsilon. J. Bacteriol. 90:1174–1181. 1965.—In the transduction of R factors with phage ε15, a lysate capable of transducing the markers for (TC) or (CM.SM.SA) resistance at high frequency was obtained. The transducing agent is a defective element called ε15dR23 which lacks certain functions of phage ε15. After lysogenization with normal ε15 phage and ultraviolet (UV) induction, strains carrying the ε15dR23 element produce lysates which have a high frequency of transduction (HFT) on group E1Salmonella. Lytic lysates prepared on phage ε15 sensitive strain with the ε15dR23 element have a low frequency of transduction (LFT). Lytic growth of phage ε34 on an ε15dR23 strain or UV induction of an ε34 lysogenic strain containing ε15dR23 results in LFT lysates on group E2Salmonella. On UV induction, group E2Salmonella (ε15 lysogens) with the ε15dR23 element give lysates which are HFT on group E1Salmonella but are LFT when tested on group E2Salmonella. In all instances, the production of drug-resistant transductants requires infection of the cell with only a single ε15dR23 element. It appears that the resistance region of the R factor has replaced that portion of phage genome which is essential for vegetative replication and superinfection immunity. The ε15dR23 element does not contain the genetic determinants of the R factor responsible for transmissibility, inhibition of F mating, and interference between two R factors.

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Selected References

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