Abstract
Sato, Ichiei (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Experimental salmonellosis. VII. In vitro transfer of cellular immunity by ribosomal fraction of mouse mononuclear phagocytes. J. Bacteriol. 90:1194–1199. 1965.—The mononuclear phagocytes (termed monocytes) of mice hyperimmunized with live vaccine of Salmonella enteritidis inhibited the intracellular growth of virulent strain 116–54 of S. enteritidis. Also, the monocytes withstood the degeneration of cells caused by the phagocytosis of bacteria in the absence of immune serum in the tissue culture medium, termed cellular immunity. When the nonimmune monocytes were incubated with the ribosomal fraction of immune monocytes, obtained from the abdominal cavity of mice hyperimmunized with live vaccine of S. enteritidis, they acquired cellular immunity, but the monocytes did not acquire immunity when ribosomal fractions from normal mouse monocytes or from the monocytes of mice immunized with killed vaccine of S. enteritidis were used. The transfer agent present in the ribosomal fraction of immune monocytes was inactivated by treatment with ribonuclease but not with deoxyribonuclease or with trypsin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- LITTLEFIELD J. W., KELLER E. B., GROSS J., ZAMECNIK P. C. Studies on cytoplasmic ribonucleoprotein particles from the liver of the rat. J Biol Chem. 1955 Nov;217(1):111–123. [PubMed] [Google Scholar]
- MITSUHASHI S., KAWAKAMI M., YAMAGUCHI Y., NAGAI M. Studies on the experimental typhoid. 1. A comparative study of living and killed vaccines against the infection of mice with S. enteritidis. Jpn J Exp Med. 1958 Aug;28(4):249–258. [PubMed] [Google Scholar]
- MITSUHASHI S., SATO I., TANAKA T. Experimental salmonellosis. Intracellular growth of Salmonella enteritidis ingested in mononuclear phagocytes of mice, and cellular basis of immunity. J Bacteriol. 1961 Jun;81:863–868. doi: 10.1128/jb.81.6.863-868.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MITSUHASHI S. [STUDIES ON CELLULAR IMMUNITY]. Arerugi. 1964 Apr;13:198–203. [PubMed] [Google Scholar]
- Mitsuhashi S., Saito K. IN VITRO TRANSFER OF CELLULAR IMMUNITY OF MOUSE PHAGOCYTES IN EXPERIMENTAL SALMONELLOSIS. J Bacteriol. 1962 Sep;84(3):592–593. doi: 10.1128/jb.84.3.592-593.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SATO I., TANAKA T., SAITO K., MITSUHASHI S. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J Bacteriol. 1962 Jun;83:1306–1312. doi: 10.1128/jb.83.6.1306-1312.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saito K., Mitsuhashi S. Experimental Salmonellosis VI. In Vitro Transfer of Cellular Immunity of Mouse Mononuclear Phagocytes. J Bacteriol. 1965 Sep;90(3):629–634. doi: 10.1128/jb.90.3.629-634.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAKANAMI M. A stable ribonucleoprotein for amino acid incorporation. Biochim Biophys Acta. 1960 Apr 8;39:318–326. doi: 10.1016/0006-3002(60)90169-4. [DOI] [PubMed] [Google Scholar]