Abstract
Kahn, Phyllis L. (Princeton University, Princeton, N.J.), and Donald R. Helinski. Interaction between colicinogenic factor V and the integrated F factor in an Hfr strain of Escherichia coli. J. Bacteriol. 90:1276–1282. 1965.—The production of colicin V by strains of Escherichia coli is determined by a colicinogenic factor, colV. The colV factor possesses a genetic determinant of fertility, Fv. V+Fv+ cells are characteristically susceptible to a male-specific phage, μ, and able to transfer the colV factor and chromosomal markers to recipient cells. The present work describes an interaction of the colV factor with the chromosome of the Hfr strain, HfrH. A colV-containing HfrH strain, designated HfrHV+Fv+(l), was isolated and shown to be insensitive to phage μ and impaired in its fertility properties. Loss of the colV factor by this strain, either spontaneous or induced by acridine orange, resulted in a further 103- or 104-fold loss in fertility. This additional loss of fertility was restored by reinfection of these strains with the colV factor. The colV interaction with the HfrH chromosome also can result in defects in the fertility properties of the colV factor. Altered colV factors were found in recombinants isolated from a cross between the HfrHV+Fv+(l) strain and F− recipients. It is postulated that in the HfrHV+Fv+(l) strain an interaction of the colV episome with the integrated F region of the chromosome occurs, with a resulting modification of the fertility properties of the HfrH strain. This interaction can also result in a defect in certain properties of the colV factor.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARADA K., KAMEDA M., SUZUKI M., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. 3. ACQUISITION OF TRANSFERABILITY OF NONTRANSMISSIBLE R(TC) FACTOR IN COOPERATION WITH F FACTOR AND FORMATION OF FR(TC). J Bacteriol. 1964 Nov;88:1257–1265. doi: 10.1128/jb.88.5.1257-1265.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAHN P., HELINSKI D. R. RELATIONSHIP BETWEEN COLICINOGENIC FACTORS E1 AND V AND AN F FACTOR IN ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1573–1579. doi: 10.1128/jb.88.6.1573-1579.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PITTARD J., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. III. AN ANALYSIS OF THE RECOMBINATION EVENTS OCCURRING IN THE F' MALE AND IN THE ZYGOTES. Genetics. 1964 Jun;49:995–1007. doi: 10.1093/genetics/49.6.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCAIFE J., GROSS J. D. Inhibition of multiplication of an Flac factor in Hfr cells of Escherichia coli K-12. Biochem Biophys Res Commun. 1962 May 11;7:403–407. doi: 10.1016/0006-291x(62)90324-8. [DOI] [PubMed] [Google Scholar]
- WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae IV. Interactions between resistance transfer factor and F-factor in Escherichia coli K-12. J Bacteriol. 1962 Apr;83:727–735. doi: 10.1128/jb.83.4.727-735.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATANABE T., FUKASAWA T., TAKANO T. Conversion of male bacteria of Escherichia coli K12 to resistance to f phages by infection with the episome "resistance transfer factor". Virology. 1962 May;17:217–219. doi: 10.1016/0042-6822(62)90108-3. [DOI] [PubMed] [Google Scholar]