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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2010 Dec 22;90(2):651–658. doi: 10.1007/s00253-010-3074-5

GGDEF Proteins YeaI, YedQ, and YfiN Reduce Early Biofilm Formation and Swimming Motility in Escherichia coli

Viviana Sanchez-Torres 1, Hongbo Hu 1,2, Thomas K Wood 1,*
PMCID: PMC3158426  NIHMSID: NIHMS315736  PMID: 21181144

Abstract

The second messenger 3′–5′-cyclic diguanylic acid (c-di-GMP) promotes biofilm formation, and c-di-GMP is synthesized by diguanylate cyclases (characterized by a GGDEF domain) and degraded by phosphodiesterases. Here, we evaluated the effect of the 12 E. coli GGDEF-only proteins on biofilm formation and motility. Deletions of the genes encoding the GGDEF proteins YeaI, YedQ, YfiN, YeaJ, and YneF increased swimming motility as expected for strains with reduced c-di-GMP. Alanine substitution in the EGEVF motif of YeaI abolished its impact on swimming motility. In addition, extracellular DNA (eDNA) was increased as expected for the deletions of yeaI (10 fold), yedQ (1.8 fold), and yfiN (3.2 fold). As a result of the significantly enhanced motility, but contrary to current models of decreased biofilm formation with decreased diguanylate cyclase activity, early biofilm formation increased dramatically for the deletions of yeaI (30 fold), yedQ (12 fold), and yfiN (18 fold). Our results indicate that YeaI, YedQ, and YfiN are active diguanylate cyclases that reduce motility, eDNA, and early biofilm formation and contrary to the current paradigm, the results indicate that c-di-GMP levels should be reduced, not increased, for initial biofilm formation so c-di-GMP levels must be regulated in a temporal fashion in biofilms.

Keywords: diguanylate cyclase, cyclic-di-GMP, biofilm, GGDEF, motility

INTRODUCTION

Bacterial cells can adopt two different lifestyles, the planktonic mode characterized by single motile cells, or the biofilm mode where bacterial cells form sedentary multicellular communities attached themselves or to a surface (Pesavento et al. 2008). The second messenger 3′–5′-cyclic diguanylic acid (c-di-GMP) acts as a switch promoting the transition from the planktonic to the biofilm lifestyle (Hengge 2009). The current c-di-GMP paradigm is that high intracellular c-di-GMP levels promote the synthesis of exopolysaccharides which are components of the biofilm matrix, promote the formation of adhesive fimbriae, and inhibit motility (Hengge 2009).

c-di-GMP is synthesized from guanosine-5′-triphosphate by diguanylate cyclases which are characterized by the GGDEF domain (Hengge 2009). Diguanylate cyclases have a conservative GG[D/E]EF motif in their active site (A-site), and some of them also have an inhibitory site for c-di-GMP binding (I-site) (Sommerfeldt et al. 2009). Degradation of c-di-GMP is catalyzed by phosphodiesterases, characterized by EAL or HD-GYP domains (Schirmer and Jenal 2009). Most bacteria have multiple diguanylate cyclases and phosphodiesterases. Escherichia coli K-12 has 12 proteins with a GGDEF domain, 10 proteins with an EAL domain, and 7 proteins with both GGDEF and EAL domains in a single polypeptide (Weber et al. 2006).

Among the 12 GGDEF-only proteins in E. coli, the diguanylate cyclase activity of AdrA (Antoniani et al. 2009), YdaM (Weber et al. 2006), YddV (Méndez-Ortiz et al. 2006), YcdT (Jonas et al. 2008), YdeH (Jonas et al. 2008), and YeaP (Ryjenkov et al. 2005) has been confirmed either in vitro, by using purified proteins, or in vivo, by measuring the effect on c-di-GMP intracellular levels after deleting or overexpressing the corresponding genes. Some of these E. coli GGDEF-only proteins alter biofilm related phenotypes since YdaM (Weber et al. 2006), YeaP (Sommerfeldt et al. 2009), and YddV (Tagliabue et al. 2010)promote curli formation, since AdrA (Antoniani et al. 2009)and YedQ (Da Re and Ghigo 2006) activate cellulose production, and since YdeH activates production of the polysaccharide adhesin poly-β-1,6-N-acetyl-glucosamine (Boehm et al. 2009). Also, swimming motility decreases by overexpressing the genes encoding YdeH and YcdT (Jonas et al. 2008). Similarly, YeaJ (Pesavento et al. 2008), YedQ (Pesavento et al. 2008), YddV (Boehm et al. 2010), and YfiN (Boehm et al. 2010) negatively regulate motility in a mutant lacking the phosphodiesterase YhjH. The phenotypes controlled by the other GGDEF-only proteins (YeaI, YliF, and YneF) have not been characterized.

Mature E. coli biofilm formation increased by overexpressing the genes encoding the GGDEF-only diguanylate cyclases AdrA (Antoniani et al. 2009), and YddV (Méndez-Ortiz et al. 2006). Hence, high c-di-GMP concentrations enhance late biofilm formation while reducing motility. However, mutants with decreased motility have reduced adhesion (Genevaux et al. 1996); hence, we reasoned that decreasing diguanylate cyclase activity should increase motility and lead to increased early biofilm formation, even though diguanylate cyclase activity is known to increase biofilm formation. To address this paradox and to investigate the role of heretofore unstudied E. coli diguanylate cyclases on biofilm formation, we compared biofilm formation of the wild-type BW25113 and its isogenic mutants defective in each of the 12 genes encoding GGDEF-only proteins and found knock-outs of the genes encoding the GGDEF proteins YeaI, YedQ, and YfiN enhanced early biofilm formation dramatically. Our results suggest that a reduction in the c-di-GMP levels caused by inactivating yeaI, yedQ, and yfiN enhances swimming motility which contributes to enhanced initial attachment to the polystyrene surface thus promoting early biofilm formation. Therefore, the current paradigm for c-di-GMP should be refined to indicate its inverse relationship to initial biofilm formation and to indicate that the timing of c-di-GMP production is likely more sophisticated than just elevated in biofilms.

MATERIALS AND METHODS

Bacterial strains, media, and growth conditions

The E. coli strains and plasmids used in this study are listed in Table 1. Single deletion mutants of the parental strain E. coli K-12 BW25113 were obtained from the Keio collection (Baba et al. 2006). The double deletion strains BW25113 yeaI yedQ and BW25113 yeaI yfiN were constructed via P1 transduction (Maeda et al. 2008). The deletions of yeaI, yedQ, and yfiN were verified as described previously (Sanchez-Torres et al. 2009) via polymerase chain reaction (PCR) using primers listed in Table 2. Primers flhDC-F2 and flhDC-R (Table 2) were used to verify via PCR that the wild -type strain and mutants used here adrA, ydaM, ycdT, yddV, ydeH, yeaP, yeaI, yeaJ, yedQ, yfiN, yliF, yneF, and ycgR do not contain IS insertions in the regulatory sequence of flhDC that may increase motility (Barker et al. 2004).

Table 1.

E. coli strains and plasmids used in this study.

Strains and plasmids Genotype/relevant characteristicsa Source
Strains
BW25113 F−Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ−rp h-1 Δ(rhaD-rhaB)568 hsdR514; parental strain for the Keio collection. Yale Coli Genetic Stock Center
BW25113 adrA (yaiC) BW25113 ΔyaiC750::kan KmR (Baba et al. 2006)
BW25113 ydaM BW25113 ΔydaM778::kan KmR (Baba et al. 2006)
BW25113 ycdT BW25113 ΔycdT771::kan KmR (Baba et al. 2006)
BW25113 yddV BW25113 ΔyddV783::kan KmR (Baba et al. 2006)
BW25113 ydeH BW25113 ΔydeH756::kan KmR (Baba et al. 2006)
BW25113 yeaP BW25113 ΔyeaP790::kan KmR (Baba et al. 2006)
BW25113 yeaI BW25113 ΔyeaI782::kan KmR (Baba et al. 2006)
BW25113 yeaJ BW25113 ΔyeaJ783::kan KmR (Baba et al. 2006)
BW25113 yedQ BW25113 ΔyedQ730::kan KmR (Baba et al. 2006)
BW25113 yfiN BW25113 ΔyfiN767::kan KmR (Baba et al. 2006)
BW25113 yliF BW25113 ΔyliF734::kan KmR (Baba et al. 2006)
BW25113 yneF BW25113 ΔyneF743::kan KmR (Baba et al. 2006)
BW25113 yeaI yedQ BW25113 ΔyeaI882 ΔyedQ730::kan KmR This study
BW25113 yeaI yfiN BW25113 ΔyeaI882 ΔyfiN767::kan KmR This study
Plasmids
pCA24N lacIq, CmR (Kitagawa et al. 2005)
pCA24N-yeaI pCA24N PT5-lac::yeaI CmR
pCA24N-yeaIE407A pCA24N PT5-lac::yeaI407 CmR; encodes YeaI with E407A This study
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a

KmR and CmR are kanamycin and chloramphenicol resistance, respectively.

Table 2.

Primers used for site-directed mutagenesis, quantitative real-time PCR (qPCR), DNA sequencing, and verification of the relevant deletions in the strains used.

Primer Sequencea
Site-directed mutagenesis at position E407 of YeaI
yeaIE407-F 5′-ATTTTAGCGCGACTGGAGGGTGCGGTGTTTGGCTTGCTATTTACC-3′
yeaIE407-R 5′-GTAAATAGCAAGCCAAACACCGCACCCTCCAGTCGCGCTAAAAT-3′
qPCR
purA-f 5′-GGGCCTGCTTATGAAGATAAAGT-3′
purA-r 5′-TCAACCACCATAGAAGTCAGGAT-3′
DNA sequencing of pCA24N-yeaIE407A
hha rear 5′-GAACAAATCCAGATGGAGTTCTGAGGTCATT-3′
Verification of strains
yeaI front 5′-GTGGCGAGAATATGAGCATCTG-3′
yeaI rev 5′-CTGGATCAGTGTACTGCCGTTA-3′
yedQ front 5′-GAGTGTCGTTGGTATGACGGTTAC-3′
yedQ rev 5′-GTTCCCAGCTAACATAGCGACT-3′
yfiN front 5′-AGTACCGCCCTACAAGAGAATG-3′
yfiN rev 5′-CAGAATACAACCGGTCAGTACG-3′
kanrev 5′-ATCACGGGTAGCCAACGCTATGTC-3′
flhDC-F2 5′-CCTGTTTCATTTTTGCTTGCTAGC-3′
flhDC-R 5′-GGAATGTTGCGCCTCACCG-3′
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a

Underlined text indicate the site-directed mutation for the codon corresponding to E407 (5′-GAG to 5′-GCG for E407A).

Experiments were conducted at 37°C in either in Luria-Bertani (LB) (Sambrook et al. 1989) or M9 minimal medium supplemented with 0.4% casamino acids (M9C). Kanamycin (50 μg/mL) was used for pre-culturing the knock-out mutants and chloramphenicol (30 μg/mL) was used for selecting plasmid pCA24N (Kitagawa et al. 2005) and its derivatives. The specific growth rates of BW25113 and the yeaI, yedQ, and yfiN knock-out mutants were measured in LB using two independent cultures for each strain with the turbidity measured at 600 nm from 0.05 to 0.7.

Crystal violet biofilm assay

Biofilm formation was assayed in 96-well polystyrene plates (Corning, Lowell, MA) as described previously (Fletcher 1977). Wells were inoculated with overnight cultures at an initial turbidity at 600 nm of 0.05 in LB and incubated for 7 h and 24 h quiescently. Biofilm formation was also assayed in M9C (Sambrook et al. 1989) for 7 h. For each strain, at least 2 independent cultures were assayed in 12 replicate wells.

Site-directed mutagenesis

The codon encoding the second glutamic acid of the EGEVF motif of YeaI GAG was mutated to the alanine codon GCG to yield a EGAVF motif. Site-directed mutagenesis was performed using pCA24N-yeaI as template with complementary primers containing the target mutation (Table 2) as described previously (Steffens and Williams 2007).

Swimming motility assay

Single colonies were inoculated onto motility plates (1% tryptone, 0.25% NaCl, and 0.3% agar) (Sperandio et al. 2002) using a toothpick. For cells with pCA24N-based plasmids, the motility plates were supplemented with 30 μg/mL chloramphenicol and 0.1 mM IPTG. The motility halos were measured after 12 h incubation and at least two independent cultures for each strain were used.

eDNA assay

eDNA was assayed as described previously using quantitative PCR (qPCR) (Ueda and Wood 2010). Briefly, LB cultures with an initial turbidity at 600 nm of 0.05 were incubated for 24 h. Supernatants (1 mL) were centrifuged for 10 min at 13 krpm to find eDNA, and the total amount of DNA in the culture (outside and inside the cells) was determined using 1 mL of culture that was sonicated for 45 s at 10 W (60 Sonic Dismembrator, Fisher Scientific Co, Pittsburgh, PA) and centrifuged at 13 krpm for 10 min. eDNA and total DNA were purified using phenol:chloroform:isoamyl alcohol (25:24:1) extraction and sodium acetate and isopropanol precipitation. eDNA and total DNA were quantified by qPCR using the StepOne™ Real-Time PCR System (Applied Biosystems, Foster City, CA) and the SuperScript™ III Platinum® SYBR® Green One-Step qRT-PCR Kit (Invitrogen, Carlsbad, CA) with primers for the reference gene purA (Table 2). At least two independent cultures were used.

Quantification of c-di-GMP

c-di-GMP was assayed as described previously using HPLC (Ueda and Wood 2009). Cells from overnight cultures were inoculated into 350 mL of LB medium and cultured for 16 h with shaking (250 rpm). Nucleotides were extracted using 65% ethanol and c-di-GMP was quantified using HPLC (Waters 515 with photodiode array detector, Milford, MA). Commercial c-di-GMP (BIOLOG Life Science Institute, Bremen, Germany) was used as the standard. The c-di-GMP peak was verified by spiking each sample with the commercial c-di-GMP, and the in vitro degradation of c-di-GMP by purified phosphodiesterase YahA was used as control.

RESULTS

Deletions of yeaI, yedQ, and yfiN increase early biofilm formation

To investigate whether reductions in diguanylate cyclase activity (which serve to decrease c-di-GMP concentrations) increase initial biofilm formation due to increased cell motility, we assayed biofilm formation after 7 h upon deleting 12 genes encoding GGDEF proteins in E. coli. In LB medium, there were not significant differences in biofilm formation between wild-type BW25113 and most of the mutants (Fig. 1a). However, three mutants increased biofilm formation dramatically: yeaI (30 fold), yedQ (12 fold), and yfiN (18 fold) (Fig. 1a). These same three mutations also increased biofilm formation in minimal medium (Fig. 1b). After 7 h of incubation, biofilm formation increased 10 fold for the yeaI mutant, 6 fold for yedQ mutant, and 18 fold for the yfiN mutant relative to the wild-type (Fig 1b). Hence, deleting yeaI, yedQ, and yfiN increase biofilm formation dramatically in both rich and minimal medium. Note there was no change in the growth rate in rich medium so these changes in biofilm formation are not related to changes in growth.

Fig. 1.

Fig. 1

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Relative normalized biofilm formation in polystyrene microtitre plates after 7 h at 37°C in LB (a) and in M9C (b). Wild-type BW25113 is shown in white; single mutants defective in genes encoding GGDEF-only proteins are in blue, the ycgR mutant, which is defective in the gene encoding the c-di-GMP regulated flagellar velocity braking protein, is red, the yfiB and yfiR mutants which lack genes in the same operon as yfiN are in green, and the double deletion mutants yeaI yedQ and yeaI yfiN are yellow. Biofilm formation (turbidity at 540 nm) was normalized by the amount of planktonic growth (turbidity at 620 nm) and is shown relative to the BW25113 normalized biofilm value. Each data point is the average of at least twelve replicate wells from two independent cultures. The error bars correspond to the standard deviation, and an asterisk indicates P-values < 0.05 using a Student’s T test.

Since the double deletion mutants yeaI yedQ and yeaI yfiN did not increase further biofilm formation (Fig. 1a), YeaI appears to regulate the same process controlled by YedQ and YfiN. In addition, after 24 h of incubation, none of the mutants significantly altered biofilm formation (data not shown). These results indicate that the deletions of yeaI, yedQ, and yfiN mainly alter the initial stages of biofilm formation which are influenced by motility (Pratt and Kolter 1998).

YfiN was first characterized by us in Pseudomonas aeruginosa where we showed it was related to rugose colony formation due to its diguanylate cyclase activity that is controlled by a tyrosine phosphatase (Ueda and Wood 2009). Since yfiN is part of the operon yfiRNB in E. coli, the biofilm formation of the yfiB and yfiR mutants was also assayed to evaluate the effect of YfiB and YfiR on the activity of YfiN. While the yfiR mutant has biofilm formation similar to the wild-type, the yfiB mutant increases biofilm formation similarly to the yfiN mutant (Fig. 1a). These results indicate that YfiB is a positive regulator of YfiN activity and that the activity of YfiN is not significantly altered by deleting yfiR.

To confirm that initial biofilm formation is enhanced when motility is not inhibited by c-di-GMP, we investigated biofilm formation with a ycgR deletion. YcgR with bound c-di-GMP inhibits swimming motility by reducing the flagella motor speed through its interaction with MotA (Boehm et al. 2010), FliG, and FliM (Paul et al. 2010). We found the ycgR mutant, like the yeaI, yedQ, and yfiN mutants, increases biofilm formation (23 fold) after 7 h (Fig. 1a) but not after 24 h (data not shown). These results suggest that the yeaI, yedQ, and yfiN deletions decrease the levels of c-di-GMP which promotes motility via YcgR inactivation thus increasing early biofilm formation.

Deletions of yeaI, yedQ, and yfiN increase swimming motility and the EGEVF motif of YeaI is necessary to reduce swimming motility

Low levels of c-di-GMP promote swimming and swarming motility (Römling and Amikam 2006); hence, we investigated swimming with the yeaI, yedQ, and yfiN mutants since inactivation of these genes should decrease c-di-GMP levels by inactivating diguanylate cyclase activity. A large increase in swimming motility was observed for the single deletions in yeaI (4 fold), yedQ (6 fold), and yfiN (10 fold) (Fig. 2). We also evaluated swimming motility for the remaining 9 mutants encoding GGDEF-only proteins and found that yeaJ (2 fold), and yneF (4 fold) also have increased motility, suggesting that the GGDEF proteins encoded by these genes are diguanylate cyclases that control swimming motility.

Fig. 2.

Fig. 2

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Swimming motility for strains with mutations in the genes encoding diguanylate cyclases YeaI (EGEVF), YedQ(GG EEF), and YfiN(GGDEF) and complementation studies for the yeaI mutant using pCA24N-yeaI producing YeaI and pCA24N-yeaIE407A that produces YeaI E407A (EGAVF). Swimming motility was assayed after 12 h at 37 °C using motility plates supplemented with 30 μg/mL Cm and 0.1 mM IPTG to induce diguanylate cyclase production from the pCA24N-based plasmids.

The increase in swimming motility phenotype caused by the yeaI deletion was complemented by plasmid pCA24N-yeaI (encoding YeaI with EGEVF) (Fig. 2). Since YeaI has a EGEVF motif instead of the conserved GG[D/E]EF motif characteristic of active diguanylate cyclases, a single amino acid change of the second glutamic acid of EGEVF (corresponding to the catalytic residue of GGDEF (Chan et al. 2004)) to alanine was introduced via site-directed mutagenesis to show that YeaI is an active diguanylate cyclase. Since motility was not complemented by pCA24N-yeaIE407A (encoding YeaI with EGAVF) (Fig. 2), the EGEVF domain of YeaI is necessary to reduce swimming motility which provides additional evidence that YeaI increases c-di-GMP as a diguanylate cyclase.

Deletions of yeaI, yedQ, and yfiN increase eDNA

eDNA is an important component of the bacterial biofilm matrix (Whitchurch et al. 2002). c-di-GMP is inversely proportional to eDNA in P. aeruginosa cultures (Ueda and Wood 2010); therefore, we investigated if the deletions of yeaI, yedQ, and yfiN alter eDNA in E. coli with the expectation that deleting these genes would reduce c-di-GMP and thereby increase eDNA. For planktonic cells cultured for 24 h in LB medium, deletion of yeaI increased eDNA 10 ± 3 fold, deletion of yedQ increased eDNA slightly (1.8 ± 0.3 fold), and deletion of yfiN increased eDNA 3.2 ± 0.1 fold. These results suggest that the mutations in yeaI, yedQ, and yfiN decrease c-di-GMP which results in increased eDNA.

Deletions of yeaI, yedQ, and yfiN do not alter the total concentration of c-di-GMP

We evaluated the c-di-GMP concentrations from cell extracts of the BW25113 and the yeaI, yedQ, and yfiN mutants from planktonic cultures in LB medium and did not find significant differences in the total concentration of c-di-GMP inside the the yeaI, yedQ, and yfiN mutants vs. wild-type BW25113. These results suggest that the effect of YeaI, YedQ, and YfiN on the c-di-GMP levels may occur locally, close to the inner membrane, since YeaI, YedQ, and YfiN are integral inner membrane proteins (Misra et al. 2005).

DISCUSSION

Biofilm formation occurs following a developmental sequence (Petrova and Sauer 2009). First, bacteria move in the liquid culture and reach a surface where cells are reversibly attached, and some cells have a strong adhesion to the surface and become sessile (Hall-Stoodley et al. 2004). These cells replicate and aggregate in a self-produced polymeric matrix thus forming a mature biofilm (Hall-Stoodley et al. 2004). Finally cells disperse from the biofilm and return to a motile state (Kaplan 2010). For all of these steps, c-di-GMP plays a role (Kaplan 2010).

In many bacteria including E. coli, high concentrations of c-di-GMP promote biofilm formation (Dow et al. 2007). For example, overexpression of the genes encoding the diguanylate cyclases AdrA (Antoniani et al. 2009) and YddV (Méndez-Ortiz et al. 2006) increase E. coli mature biofilm formation. c-di-GMP also inversely regulates motility (Méndez-Ortiz et al. 2006), and motility is important for initial attachment to a surface; non-flagellated cells or cells with paralyzed flagella have reduced initial biofilm formation (Pratt and Kolter 1998), and E. coli strains with high motility make more biofilm than strains with poor motility (Wood et al. 2006). Motility also affects biofilm architecture since biofilms of strains with high motility make vertical structures while strains with poor motility form flat biofilms (Wood et al. 2006). Hence, opposite to the current understanding that c-di-GMP promotes biofilm formation, we hypothesized that deletion of the genes encoding diguanylate cyclases should decrease c-di-GMP levels thus increasing motility and early biofilm formation. Although we found a significant reduction in early biofilm formation for yddV (4 fold), deletions of yeaI, yedQ, and yfiN increased dramatically biofilm formation after 7 h of incubation in LB at 37°C (Fig. 1) while increasing motility (Fig. 2). These results suggest that the yeaI, yedQ, and yfiN mutations affect the initial steps of biofilm formation by decreasing c-di-GMP which results in higher motility. Hence, c-di-GMP levels should be low for initial biofilm formation. Furthermore, predicted inner membrane proteins (Misra et al. 2005) YeaI, YedQ, and YfiN function as active diguanylate cyclases producing c-di-GMP.

Previous reports indicated that single deletions of genes encoding E. coli GGDEF proteins do not have a much of an effect on motility (Jonas et al. 2008; Boehm et al. 2010) unless the gene encoding the phosphodiesterase YhjH is inactivated (Pesavento et al. 2008; Boehm et al. 2010). In contrast, we found a dramatic increase in motility after 11 h of incubation at 37°C for the knock-out mutants of genes encoding the GGDEF proteins YeaI, YedQ, YfiN (Fig. 2), YeaJ, and YneF. These increases in motility combined with an increase in initial biofilm formation were corroborated by an increase in biofilm formation by the ycgR strain which lacks the YcgR motility brake that is activated by c-di-GMP (Fig. 1).

eDNA is required for initial attachment to a surface and has a structural role connecting the cells in the biofilms (Rice et al. 2007). Previously, we reported that in P. aeruginosa eDNA is inversely regulated by c-di-GMP (Ueda and Wood 2010). To determine if the same eDNA regulation occurs in E. coli, we evaluated eDNA for the yeaI, yedQ, and yfiN mutants and found that eDNA increases as expected for low c-di-GMP levels. Recently, we reported that deletion of hns (encoding the global regulator H-NS) abolished eDNA production in E. coli (Sanchez-Torres et al. 2010). The hns mutant is also defective in swimming motility (Ko and Park 2000). Since inactivation of ycgR (encodes a motility brake) and overexpression of yhjH (encodes a phosphodiesterase) restore the motility defect of the hns mutant (Ko and Park 2000), the hns deletion may increase c-di-GMP levels thus inhibiting motility via YcgR. Hence, our results for yeaI, yedQ, yfiN, and hns mutants suggest that c-di-GMP negatively regulate eDNA production in E. coli.

Our results suggest that the network of diguanylate cyclases and phosphodiesterases in E. coli tune the c-di-GMP concentrations according to the developmental sequence of biofilm formation. Initially, c-di-GMP concentrations should be low to promote early biofilm through increased swimming motility and increased eDNA as reported here for yeaI, yedQ, and yfiN, then c-di-GMP should be increased to promote biofilm maturation by inducing exopolysacharide production, formation of adhesive fimbriae, and sessility. Finally, c-di-GMP should decrease to produce biofilm dispersal through higher motility as we reported recently (Ma et al. 2010).

Acknowledgments

This research was supported by the National Institutes of Health (R01 GM089999). We are grateful for the KEIO and ASKA strains provided by the Genome Analysis Project in Japan and for the collaboration of Prof. Toshinari Maeda in the construction of the yeaI yedQ and yeaI yfiN strain s.

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